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Citations for Merck :
2301 - 2350 of 2677 citations for 6 Phenylamino 2 3 dihydro 1H indolizin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and Thecadinium kofoidii were harvested during late exponential growth phase by filtration onto 5 µm SMWP Millipore membrane filters (Merck, DE) and washed off with sterile seawater ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
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bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Bioengineering 2021Quote: ... INS-1 cells in bioprinted grid scaffolds were stained with thiazolyl blue tetrazolium bromide (MTT) after 5 days in culture according to the manufacturer’s protocol (Merck, Darmstadt, Germany). Proliferation was determined using an ATP-based assay with luminescent readout (CellTiter-Glo® 3D Cell Viability Assay ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 15 minutes in 95% and 100% ethanol and 5 minutes in hexamethyldisilazane (HMDS, Sigma-Aldrich, Merck, St Louis, USA). Samples were left to air-dry overnight in a biosafety cabinet prior to mounting on electron microscope stubs covered with carbon tape ...
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bioRxiv - Microbiology 2021Quote: ... The diluted solution was concentrated to approximately 5 mL using 30 kDa molecular weight cutoff Amicon Ultra centrifugal filters (Merck KGaA), passed through a Millex-GP syringe filter with 0.22 μm pore size (Merck KGaA) ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Microbiology 2020Quote: ... column using a nanoUPLC (nanoLC Ultra 1D plus, Eksigent, USA) and eluted (400 nL/min) with acetonitrile gradient (5%-80%) (LiChrosolv®, Merck) with 0.1% formic acid ...
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bioRxiv - Immunology 2020Quote: ... The pseudo-particle-containing supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... the HA-GlmS line was transfected with the pHGBrHrBl-1/2 construct in order to episomally express cytosolic GFP then selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). To generate Pfcerli2SLI-TGD ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and SsuT3-oE-R (5′-AGTCAG GGATCC CTA CAC CAC CTT CAC TTT GGT ACC) with KOD Hot Start DNA polymerase (Merck Millipore) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore). HEK-293 cells stably expressing hACE2 were seeded into 0.1% gelatin-coated 96-well plates (Greiner ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Molecular Biology 2019Quote: ... samples were immersed in xylene (ten successive immersions for 5 minutes each) and mounted on slides cover-slipped using Entellan mounting medium (Merck, Millipore). Images were acquired using on Axio microscope with 20x/0.35 and 40x/0.55 Zeiss A-Plan objectives (Carl Zeiss ...
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bioRxiv - Immunology 2019Quote: ... and viral titers were determined in Vero cells using a standard plaque assay at day 5 post-infection with crystal violet staining (Merck Millipore). The viral titers were determined in aliquots of harvested medium ...
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bioRxiv - Systems Biology 2020Quote: TGF-β/Activin/Nodal signal inhibitor (SB) stock (4–5 mM): SB 431542 hydrate (#S4317-5MG, Merck & Co., Inc., NJ, USA) diluted in DMSO (#D2650-5×5ML ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated and diafiltrated against 20 mM phosphate buffer containing 500 mM NaCl and 20 mM imidazole (pH 7.4) using a Labscale TFF system equipped with a 5 kDa cut-off PelliconTM XL device (Merck Millipore, PXC005C50). His-tagged trimer Spike and monomeric RBD were captured using a 5 mL HisTrap FF crude column (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... for 30 min at 37 °C and washed with 5% FBS (EmbryoMax® ES Cell Qualified FBS, ES-009-B, Merck) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... to remove larger organisms and was filtered on board first through 5 μm and then through 0.2 μm polycarbonate filters (47 mm, Merck, Darmstadt, Germany) using pressurized air ...
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bioRxiv - Microbiology 2021Quote: ... and the remaining sample was centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2021Quote: ... they were centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2019Quote: ... Metabolite separation was performed by injecting 7 µL of the extract into a SeQuant® ZIC-pHILIC PEEK coated column (150 mm × 4.6 mm, 5 µm polymer, Merck Millipore) maintained at 25°C ...
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bioRxiv - Immunology 2022Quote: ... The remaining volume was spun down at 500x g for 5 minutes and resuspended in 12 ml FACS buffer (PBS [Gibco]+ 0.5% BSA [Merck] + 2mM EDTA). The suspension was transferred over a 70 μm SmartStrainer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation 100 μL of 2X STOP buffer (NaCl 340 mM, EDTA 20 mM, EGTA 4 mM, 5% Digitonin, 100 μg/mL RNase A (Merck, R6513), 50 μg/mL Glycogen (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml min−1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC system Shimadzu Prominence (Shimadzu, Kyoto, Japan) was used with Chromolith 100 (5 μm) RP-18 endcapped column (Merck, Darmstadt, Germany), mobile phase was 60% methanol ...
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Biophysics 2023Quote: ... were cleaned to remove any contaminants by sonication for 5 minutes in 50/50 isopropanol and Milli-Q water (Merck, Germany), followed by 5 minutes in Milli-Q only ...
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bioRxiv - Biophysics 2023Quote: Rec samples were transferred to a labeling buffer (50 mM HEPES, pH=7.0, 5 mM EGTA) using 10 kDa MWCO Amicon Ultra centrifugal filter unit (Merck Millipore, USA), and concentrated to 0.5 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... The mixed solution was centrifuged at 4500 rpm for 5 minutes to keep the pellet which was resuspended and washed twice with 500 μL of PBS 1X (Merck, Germany) to finally add 200 μL of PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Microbiology 2022Quote: ... The AGA52 was grown in MRS broth (0.25% dextrose + 0.3% ox bile containing) supplemented with 100ppm cholesterol (5-cholesten-3β-ol (Sigma, Merck GmbH, Darmstadt, Germany), dissolved in 2-propanol ...
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bioRxiv - Genomics 2023Quote: ... 20 µL of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2x final concentration of Protease Inhibitor Cocktail Set 1 (Merck Chemicals). After centrifugation (5 min 15000g) ...
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bioRxiv - Bioengineering 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA) a constant flow rate of 0.3 ml/min with mobile phase A being 0.1 % Formic acid in 99:1 water:acetonitrile (Honeywell ...
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bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue fragments (‘organoids’) released were incubated for 5 min in Red Blood Cell Lysis buffer (Merck Sigma-Aldrich, Gillingham, Dorset, UK), washed and then plated for 1 hr at 37°C in DMEM/10%FBS (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... We performed the hybridization reaction by mixing 10 nM scaffold DNA with 15× surplus of unmodified staple strands and 30× surplus of modified staple strands in hybridization buffer (5 mM tris(hydroxymethyl)aminomethane (TRIS) (Merck, 1.08382.2500), 5 mM sodium chloride (NaCl ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...