Labshake search
Citations for Merck :
2101 - 2150 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... coated dishes at low confluency (5,000–10,000 cells/cm2) in MEF-conditioned hES medium supplemented with 10 μM ROCK inhibitor (Y-27632; Merck, Cat#688000). On DIV0 of the differentiation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of gDNA from individual GCs (2 ng input) was performed with1 ul of JH reverse primer (10 uM, provided by MERCK) and 1.8 ul of LD forward primer set pools (10 uM per primer ...
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bioRxiv - Plant Biology 2023Quote: ... Sheared chromatin was incubated with 10 µg of α-H4.V or 5 µg of α-H4K5Ac (Merck 07-327) or 4 µg of α-H4 (Abcam ab10158 ...
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bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
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bioRxiv - Biophysics 2023Quote: Samples for native mass spectrometry were exchanged into 50 mM ammonium acetate (pH 6.8) using 10 kDa cut-off Amicon Ultra centrifugal filtration units (Merck Millipore). Samples were introduced to a first-generation Waters Synapt QToF (Waters Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells from identical conditions were collected and pooled in 10 ul in calcium-free and magnesium-free HBSS (Merck, H8264) + 50% FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Preparative scale reactions were carried out in 3 mL Slide-A-Lyzer cassettes (10 kDa MWCO, Merck Millipore, Darmstadt, Germany) in combination with custom-made FM containers57 ...
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bioRxiv - Microbiology 2023Quote: ... The cell suspensions were centrifuged at 10,000 x g for 10 minutes and bacterial pellets were lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Immunology 2023Quote: ... Both the native fab and the deglycosylated fab were buffer exchanged into 150 mM ammonium acetate (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore). The samples were loaded into gold-coated borosilicate capillaries (in-house prepared ...
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bioRxiv - Cell Biology 2023Quote: Whole liver lysates and lipid droplet-enriched fraction proteins were loaded on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Merck). Membranes were incubated at room temperature for 1 h with blocking buffer (TBS ...
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bioRxiv - Biophysics 2022Quote: ... The eluted protein was buffer exchanged with PBS (pH 7.4) and was concentrated using 10 kDa cut off Amicon (Merck Millipore) concentrator to a final concentration of 1 mg/ml and stored at −80°C until further use.
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bioRxiv - Immunology 2023Quote: ... WT mice were 7-8 week-old Mice (C57BL/6 WT or Nr4a3-Timer-GS) were injected with 10 mg/kg azoxymethane (Merck) via i.p ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed in PBS and then transferred in a line on the glass chamber between electrodes filled with PBS containing 10 mM BrUTP (Merck). The poring pulse (voltage ...
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bioRxiv - Cell Biology 2023Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Pathology 2023Quote: ... lobus accessorius) were collected and fixed in 10 % neutral-buffered formalin (Chemie Vetrieb GmbH & Co) or 5 % glutaraldehyde (Merck KGaA) for microscopic and ultrastructural evaluation respectively.
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bioRxiv - Microbiology 2023Quote: ... 15 mL of the filtered culture supernatant was first concentrated using Amicon Ultra 15 mL Centrifugal Filters (cut-off 10 kDa, Merck), and the proteins were precipitated out of the solution using trichloroacetic acid (TCA ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated in a buffer containing 20 mM HEPES-KOH (pH 7.5) and 0.2% Noidet P-40 using a dialysis spin column (Amicon Ultra-10; Merck, Darmstadt, Germany). The concentrations of purified fluorescent proteins were measured using fluorescence correlation spectroscopy (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Once DRGs were removed and placed in ice-cold neuronal growth medium (Neurobasal medium, B27, and 10 ng/ml Nerve growth factor (NGF)-2.5 s (Merck Millipore), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... tuberculosis GlnR-TAC were concentrated to 40 mg/mL using Amicon Ultra centrifugal filters (10 kDa MWCO, Merck Millipore, Inc.). Sequences of the above amtB oligonucleotides are shown in Supplementary Table S1.
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants (35 mL) were removed at day 6 and day 10 and filtered through a Miracloth (Merck Milipore, Carrigtwohill, Ireland) into a clean tube ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Biochemistry 2023Quote: ... the reduced cysteine residues were blocked employing a 10 mM solution of S-methyl methanethiosulfonate (MMTS) (Merck KGaA, Darmstadt, Germany) at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were lysed in a protein extraction solution (∼10 µL/ mg tissue) consisting of 0.32M sucrose with protease inhibitors (Merck #4693159001) and phosphatase inhibitors (Pierce # A32957 ...
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bioRxiv - Cell Biology 2023Quote: ... Conditioned media were collected after 48 h and centrifuged at 3,000 g for 10 minutes before passing through a 0.22 μm Millex® syringe filters (Merck Millipore). For viral infection ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated for 10 minutes at 37°C with Liberase (250 μg/ml) and DNAse (50μg/ml Merck®). After incubation ...
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bioRxiv - Microbiology 2024Quote: ... 10 μM of BPP43_05035 was subjected to incubation with 0.5% porcine gastric mucin II or III (Merck, #M2378 and #M1778) in reactions conducted under pH 5.5-8.5 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were dehydrated by incubating them with mild rocking for 10 minutes each with increasing concentrations of absolute ethanol (Merck) in water from 25% to 100% ethanol (v/v) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 µL of the washed GUV solution was transferred to an 18-well imaging chamber and supplemented with 10 mM Magnesium-Ionophor I (Merck). Once the sample was placed on the confocal microscope with an incubation chamber held at 37 ◦C for 2 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The interlocking ring included a central odorant delivery platform comprising a 10-mm-diameter cover glass and two 5-mm-diameter filter discs (WHA10016508; Merck) for the evaluation of VOCs (stl files are provided in Extended Data Fig ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were then transferred to cell-culture plastic plates that had been previously treated with poly-L-lysine 10 μg/ml (Sigma-Merck) to enhance cellular adhesion and promote monolayer cultures ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-20 µg protein lysate were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) using 8-10% gels and transferred to Amersham Protran 0.45 µm nitrocellulose membranes (Merck, #GE10600012) by wet blotting using the Mini-Protean Vertical Electrophoresis system (Bio- Rad) ...
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bioRxiv - Biophysics 2024Quote: ... HeLa cells were cultivated at 37 °C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Molecular Biology 2024Quote: ... Animals that were collected on day 2 of adulthood were transferred to plates containing 5-FU (10 µM) (Merck, #F6627) at the L4 larval stage to prevent progeny production ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction was quenched with three subsequent washes using the quenching buffer described in Loh et al.24 based on Tyrode’s solution supplemented with 10 mM sodium azide (Merck, S2002), 10 mM sodium L-ascorbate (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... The eluted protein was concentrated with Amicon Ultra 15 centrifugal filters (Regenerated cellulose 10 000 NMWL; Merck Millipore, Darmstadt, Germany), aliquoted and flash-frozen on dry ice and stored at −80°C until use ...
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bioRxiv - Neuroscience 2024Quote: ... sections were permeabilized for 1h in 1X PBS containing 0.3% or 0.15% Triton X-100 and 10% donkey serum (Merck, #S30-100ml). Thereafter ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was then collected from the GSH beads and was concentrated using centrifugal filters with a 10 kDa mass cut-off (Merck). The concentrated protein sample was further purified using a Superdex 200 16/600 size-exclusion column ...
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bioRxiv - Biochemistry 2024Quote: ... for 5-10 min at room temperature to reveal bound cytokine and reaction was blocked by adding HCl 2N (Merck) solution (20 μL/well) ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 5-10 μg of primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) in 250 μL LB3 with 1% Triton X-100 overnight at 4 °C ...
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bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
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bioRxiv - Biochemistry 2021Quote: ... The protein complexes were concentrated to 0.7-1 mg ml-1 using an Amicon Ultra-0.5 mL concentrating device (Merck) with a 100 kDa filter cut-off ...
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bioRxiv - Genetics 2019Quote: ... These were then re-solubilized in 30μl chloroform/methanol (1:1) and loaded onto silica gel plates (Merck). PA and DAG were separated by 10-15min of thin layer chromatography (TLC ...
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bioRxiv - Microbiology 2019Quote: ... Antibody staining was performed using EV-A71 monoclonal antibody MAB979 (Merck, USA; 1:2500 dilution in 1 % BSA) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM EDTA and 1 mM DTT and concentrated with Amicon Ultra-0.5 mL centrifugal filter (Merck #UFC503096). Nucleosomes were stored in 4 °C and the quality of nucleosomes was assessed by 5-6 % TBE gel.
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:1 dilution with tris-buffered saline (TBS) containing 0.1% (v/v) Tween® 20 (Merck). Subsequently ...
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bioRxiv - Biophysics 2022Quote: ... All pH adjustments were performed using 1 M HCl or 1 M NaOH (99.0 %, Merck KGaA, Darmstadt, Germany). All samples were filtered prior to stress application through a 0.1 µm inorganic membrane filter (#6809-2012 ...
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bioRxiv - Immunology 2020Quote: ... rAAVDJ or rAAV6 genome plasmid and Donor plasmid at a 3:1:1 ratio in Polyethylenimine (PEI)(Merck). In total each plate was teransfected with 41,250ng of DNA ...