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Citations for Merck :
2101 - 2150 of 5243 citations for 8 4 ethoxy 3 methoxyphenyl 1 5 diazabicyclo 3.2.1 octane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... mouse anti-Centrin (1:500-1:1000, Merck Millipore), chicken anti-GFP (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM AEBSF) and 1 protease inhibitor tablet (Merck). Lysis was done at 4 °C with the addition of 1 mg/ml lysozyme and further incubation at 4 °C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... anti-α-tubulin (Merck, 1:1000, TAT-1 00020911), anti-GFP (ChromoTek ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µM StemReginin 1 (SR1, Merck Millipore, 182706) for the indicated time points.
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bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... and 10% ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... [2H7]22R-Hydroxycholest-4-en-3-one ([2H7]22R- HCO) was prepared from [2H7]22R-HC by treatment with cholesterol oxidase enzyme (Streptomyces sp., Merck, Dorset, UK) (11).
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bioRxiv - Microbiology 2021Quote: ... and 10 % Ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Physiology 2022Quote: ... and separation was carried out at 40°C using a ZIC-HILIC column (2.1 mm × 100 mm, 3 μm; Merck Sequant, Umea, Sweden). Isocratic elution was performed ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Genomics 2024Quote: ... Two million NIH-3T3 and K562 cells were collected and fixed in a 3 % glyoxal solution (40 % glyoxal (Merck, catalog no. 128465), 0.75 % acetic acid ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies (FUS 1:100 Santa Cruz Biotech sc-47711; ISL1 1:500 Abcam ab109517; OLIG2 1:100 R&D Systems AF2418; NFM 1:1000 Merck MAB1621) were added in 1% BSA and incubated at 4degC overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Biophysics 2019Quote: ... fractions containing monomeric PSCFP2*-AVI-3C-12xHis were concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) in the presence of 100 µM Tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Microbiology 2021Quote: ... containing a knockout of DNA Ligase 4) 44 that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT; Merck). The transformed cells were plated on minimal synthetic defined (SD ...
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bioRxiv - Biophysics 2020Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with 10kDa Amicon®Ultra-4 centrifugal filters (MERCK). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... The resulting 5.6-mL fraction was concentrated to 250 μL by ultrafiltration using Amicon Ultra-4 10K centrifugal filters (Merck, USA), then submitted to immunodetection.
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further separated by size exclusion chromatography (HiLoad 26/600 Superdex 75, Cytiva) and concentrated using Ultra-4 or 15 mL centrifugal filter devices (Amicon, Merck). Correct size and high purity were verified via SDS-PAGE and LC-MS analysis ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were washed ten times in PBS and ten times in ultrapure water before mounting on slides using Mowiol 4–88 (Merck) containing 200 nM 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cell Biology 2022Quote: ... Filters were incubated in a cold room (4°C) overnight with primary antibodies diluted in PBS with 0.1% TWEEN® 20 (Merck) solution (1:1000 dilution) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Systems Biology 2022Quote: ... The samples are placed in 3k cut-off centrifugal filters (Amicon Ultra −4 Centrifugal filters, Merck Millipore Ltd Cork, Ireland) and spun down at 4500 rpm for 25 minutes using a benchtop centrifuge ...
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bioRxiv - Molecular Biology 2020Quote: ... with 1 % bovine serum albumin (BSA) and incubated overnight at 4 □ in TTBS with 0.1% BSA and the following primary antibodies: mPer1 (#AB2201, Merck Millipore), PER2 (#AB2202 ...
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bioRxiv - Cancer Biology 2019Quote: 3D invasion cultures were washed with phosphate buffered saline pH 7.4 (PBS) once after removing medium and fixed with 4% neutral buffered formaldehyde (Merck, 1.94989.0521) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% (vol/vol) paraformaldehyde for 10 min at RT and permeabilized with 0.3% Tween 20 (#822184, Merck Millipore). All the primary and secondary antibodies were diluted in blocking buffer consisting of 0.3 M glycine ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin and its associated proteins were solubilized by incubation with 4- 20 units of Micrococcal nuclease (MNase; Merck Life Sciences) for 10 minutes at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 600 ng DNA (per well of a 4-well LabTek dish) using 0.75 μl Genejuice (Merck Millipore) following the manufacturer’s protocol ...