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Citations for Merck :
2351 - 2400 of 5243 citations for 8 4 ethoxy 3 methoxyphenyl 1 5 diazabicyclo 3.2.1 octane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... The HPLC setup consisted of a ZIC-pHILIC column (SeQuant, 150 mm x 2.1 mm; Merck KGaA, 5 mm), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were then permeabilized in blocking solution containing 5% normal goat serum (NGS, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in 0.5% Triton for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... and 5 µl aliquots of the supernatants were applied to polyethyleneimine (PEI) cellulose thin layer chromatography plates (Merck, Sigma), resolved with 1.5 M KH2PO4 (pH 3.4 ...
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bioRxiv - Microbiology 2019Quote: ... and two zwitterionic functional groups – sulfoalkylbetaine bonded to (IV.) polymer support (ZIC-pHILIC, 100 × 4.6 mm, 5 µm; Merck) or (V. ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Pathology 2021Quote: ... hippocampal extracts prepared as described above were boiled for 5 min in 50 mM Tris-HCl pH 8.0 containing 12% Glicerol (Merck), 4% SDS ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... control cells were generated using the SHC202 -MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid (Merck). After infection ...
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bioRxiv - Microbiology 2021Quote: ... USA) at a multiplicity of infection (MOI) of 5–10 in 1.5 mL Ex-Cell 405 medium (Merck, Germany), and the infected cells were incubated at 26 °C ...
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bioRxiv - Microbiology 2020Quote: ... The homogenate was centrifuged at 10,000× g for 5 min and the supernatant was filtered through a 0.45 μm filter (Merck Millipore) to remove large particles ...
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bioRxiv - Immunology 2020Quote: ... conditional knock out) allows deletion of Csf1r following treatment with the tetracycline analog doxycyclinehyclate (2μg/ml in 5% sucrose water; Merck) causing CSF1R expressing macrophage populations to be depleted ...
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bioRxiv - Microbiology 2020Quote: ... live cell fluorescence microscopy was performed as described before (85) with minor modification using 5 μg/ml Hoechst (Merck) to stain the nuclei ...
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bioRxiv - Immunology 2022Quote: Lungs were cut into 5 mm3 cubes and digested in 12 ml of collagenase I (2.4 mg/ml, Merck) and DNase I (1 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted metabolites were separated on a SeQuant ZIC-pHILIC column (2.1 × 150 mm, 5 μm bead size, Merck Millipore). Flow rate was set to 0.2 mL/min ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured for at least 5 passages in DMEM containing either L- arginine and L-lysine (Merck) or L-arginine [13C6] and L-lysine [2H4] (Cambridge Isotope Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were then analysed by LC-MS by separating using hydrophilic interaction liquid chromatography with a SeQuant ZIC-pHILIC column (2.1 × 150 mm, 5 μm) (Merck). Analytes were detected with high-resolution ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein pellets were resuspended in 1x S-Trap lysis buffer (5% SDS, 50 mM TEAB from Merck, pH 8.5). 50 µg of the sample protein was digested using the S-trap micro spin column digestion protocol from ProtiFi LLC ...
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bioRxiv - Microbiology 2024Quote: ... Chromatography was performed on an Agilent Infinity II 1290 HPLC system using a SeQuant ZIC-pHILIC column (150 × 2.1 mm, 5 μm particle size, peek coated, Merck) connected to a guard column of similar specificity (20 × 2.1 mm ...
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bioRxiv - Microbiology 2024Quote: The chromatographic separation for metabolite profiling was performed using a SeQuant ZIC-pHILIC (2.1×100 mm, 5-μm particle) column (Merck) at 40°C ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... 400 μg of AtLEGβ or papain were inhibited with 0.5 mM or 5 mM S-methyl methanethiosulfonate (MMTS, Merck), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was enzymatically sheared for 5 min at 37°C with 0.2 mU/μL Micrococcal nuclease (MNase) (#N3755, Merck), and stopped with MNase stop buffer (110 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 20-100 µl 70% acetonitrile in water and run on a ThermoFisher Q-Exactive Orbitrap mass spectrometer equipped with Zic-pHILIC column (150×2.1 mm, 5 mm; Merck). A volume of 5 µl was injected and metabolites were monitored in full-scan ...
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bioRxiv - Molecular Biology 2023Quote: ... The formula was build based on a twofold serious dilution of glycerol (>/= 99% purification, Merck, cat # 56-81-5). The glycerol density (microgram glycerol/mg hyphal weight ...
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bioRxiv - Microbiology 2023Quote: ... and SAM_Seq_Ex8_RV (5’-CTT CTT ATT GCC TCC TCT GGC ACA GC) together with KOD Hot Start DNA Polymerase (Merck) or KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies from those plates were inoculated in 5 mL of tryptic soy broth (TSB; Merck KGaA, Darmstadt, Germany) and incubated for 16 - 18 h at 37°C with shaking at 200 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... rinsed for 5 min in water and then placed for 30 min in the Schiff reagent (Merck, Darmstadt, Germany). After three 2-min washes in water ...
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bioRxiv - Immunology 2023Quote: Cells were infected separately with five different lentiviral transduction particles (at MOI = 5) containing five different shRNA species (Merck) specific for the mouse Flot2 gene (NM_008028 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... The onion cells were prepared by dissecting a 5 mm x 5 mm section of the abaxial epidermis from a brown onion and mounting the membrane (5 mm x 5 mm) onto a #1.5 coverslip with 100 µl of neat Lugol’s iodine solution (62650; Merck, Germany) to produce a stained specimen.
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bioRxiv - Neuroscience 2023Quote: ... LC-MS analysis was performed with a ZIC-pHILIC chromatographic column (5 µm, 2.1 × 150 mm, Merck, Darmstadt, Germany). Full scan positive and negative ionization modes with a resolution of 70.000 (FWHM ...
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bioRxiv - Microbiology 2023Quote: ... Chromatographic separation was performed on a SeQuant ZIC-HILIC column (5 µm, 200 Å, 150 × 2.1 mm, Merck, Germany) equipped with a SeQuant ZIC-HILIC guard column (20 × 2.1 mm ...
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bioRxiv - Biophysics 2023Quote: ... 20 µL 5% w/v solution of polyvinyl alcohol (PVA; fully hydrolyzed, MW = 145000 Da; Merck Group, Darmstadt, Germany) in water with 50 mM sucrose was spread onto a 2 cm by 5 cm area corresponding to the dimensions of a rectangular ...
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bioRxiv - Immunology 2024Quote: ... followed by centrifugation at 1,000 rpm for 5 min and filtration using 0.45 μm sterile syringe filters (Merck Millipore). The filtrate was then concentrated with a 100 kD Amicon ultra centrifugal filter unit (Merck Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... whose composition is as plating media but with 5% FBS and 2 μM cytosine β-d-arabinofuranoside (Merck, C6645) to limit glial growth ...
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bioRxiv - Cancer Biology 2024Quote: ... were separated using a ZIC-pHILIC guard and analytical column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck) on an Ultimate 3000 high-performance liquid chromatography (HPLC ...
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bioRxiv - Cancer Biology 2024Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Biochemistry 2024Quote: ... The chromatographic separation was performed on an Agilent Infinity II 1290 HPLC system using a SeQuant ZIC-pHILIC column (150 × 2.1 mm, 5 μm particle size, peek coated, Merck) connected to a guard column of similar specificity (20 × 2.1 mm ...
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bioRxiv - Immunology 2024Quote: B cell lines used as target cell lines (i.e. Raji and MEC1) were grown at 37°C and 5% CO2 in RMPI-1640 medium (Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone).
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bioRxiv - Cell Biology 2020Quote: ... permeabilized in 1% DMSO/1% Triton X-100 and stained with anti-phospho H3 antibody (1:250, Merck Millipore) followed by anti-rabbit Alexa 488 secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL lysis buffer (15 mM NaHEPES pH 7.3, 30 mM NaCl, 1 mM MgCl2, 1% Brij35, 1 U/µL benzonase (Merck), 1X complete protease inhibitor (EDTA-free ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HPLC purification of the (S,S)-MLN-4760 was based on the comparison with the commercially available compound (MLN-4760; MW: 428.31; Merck, CAS N° 305335-31-3). The isolation of the desired (S,S)-diastereoisomers of the respective fluorinated MLN-4760 derivatives was based on the assumption that the HPLC elution sequence of the diastereoisomers would be identical to that of the MLN-4760 lead compound.
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Immunology 2024Quote: ... the wellbeing of the fish was followed at least once a day and fish exhibiting symptoms, signs of pain or discomfort were euthanised with an overdose of a Tricaine anaesthetic (Ethyl 3-aminobenzoate methanesulfonate, (Merck, Kenilworth, New Jersey, USA)) ...
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bioRxiv - Microbiology 2021Quote: ... The suspension was treated with DNase I (1 μg ml-1) and RNase A (100 μg ml-1, Merck, India) to degrade nucleic acid residues of the host bacteria ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in 8 mL lysis buffer (5 mM imidazole in 100 mM potassium phosphate buffer pH 8.0) supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-PSD-95 (Merck Millipore, cat 04-1066, 1:200; 1:1,000); anti-vGlut (Merck Millipore ...