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Citations for Merck :
2101 - 2150 of 2734 citations for 6 4 OXO 2 THIOXO THIAZOLIDIN 3 YL HEXANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was then spun at 20,000 g for 20 min at 4°C and loaded to a HisTrap HP column (Merck). The protein was eluted with the buffer with 300 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Calibration curves were acquired using defined amounts of the fluorescent product 4-methylumbelliferone sodium salt (#M1508, Sigma-Aldrich/Merck). Fluorescence of 4-methylumbelliferone was normalized to crystal violet intensity.
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration (4°C, 3,500 x g) using Amicon Ultra Centrifugal Filter Units 10K molecular weight cut-off (MWCO) (Merck). CT produced in E ...
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bioRxiv - Developmental Biology 2023Quote: Axolotl embryos of genotype tgSceI(Mmu.Prrx1:TFPnls-T2A-ERT2-Cre-ERT2; Caggs:loxP-GFP -loxP-mCherry)Etnka were treated with 4-OHT (Merck H7904) as described in 48 to permanently label connective tissue cells with mCherry ...
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bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
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bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
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bioRxiv - Genomics 2024Quote: ... media was changed for 500 µL of DMEM +5% FBS +4 μg/mL of polybrene (Merck TR-1003-G). Lentiviruses were diluted to the desired MOI in 500 µL of DMEM +5% FBS and slowly added to each well ...
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bioRxiv - Biophysics 2019Quote: ... for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO, Amicon, Merck Millipore) into storage buffer ...
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bioRxiv - Molecular Biology 2021Quote: WT MtbRho and the M495L mutant were overexpressed in Rosetta 2(DE3) cells (Merck-Millipore) harboring the appropriate pET28b derivative and purified following published protocols35 with minor modifications ...
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bioRxiv - Developmental Biology 2019Quote: ... The next day FGF ligands (to 5 nM) and heparin (to 2 μg/ml; Merck) were added ...
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bioRxiv - Biochemistry 2020Quote: ... and a SeQuant ZIC-HILIC precolumn (5 μm particles, 20 × 2 mm) (Merck, Darmstadt, Germany) using a linear gradient of mobile phase A (5 mM NH4OAc in acetonitrile/H2O (5/95 ...
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bioRxiv - Biochemistry 2021Quote: Ethanol (absolute for analysis) and 2-propanol (for analysis) were obtained from Merck (Darmstadt, Germany). Sodium chloride (≥99.8%) ...
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bioRxiv - Cell Biology 2021Quote: ... Intracellularly applied drug: the membrane impermeable G-protein blocker GDP-β-S (2 mM, Merck) (Farkas et al. ...
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bioRxiv - Cell Biology 2020Quote: ... at a final concentration of 10 ng/ml and phosphatase inhibitor cocktail-2 (P5726, Merck) at 1% (v/v) ...
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bioRxiv - Immunology 2022Quote: ... EB retained in the brain tissue was extracted by immersion in 2 ml formamide (Merck) at 37°C in the dark for 48 h ...
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bioRxiv - Biochemistry 2022Quote: ... and the cells grown in 2 L of Overnight Express Instant LB medium (Merck, Germany) containing 100 μg/ml ampicillin at 28 °C for approximately 32 h ...
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bioRxiv - Microbiology 2022Quote: ... diluted in RPMI 1640 medium supplemented 2 g/L of NaHCO3 (Merck®, Burlington, MA) and 10% fetal bovine serum (Gibco® ...
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bioRxiv - Biophysics 2022Quote: ... bis-acrylamide (2% w/w) and ammonium persulfate (0.05% w/v) (all from Merck, Germany) in 10 mM Tris-buffer (pH 7.48) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell-free samples were fixed for 1 hour at room temperature with 2% glutaraldehyde (Merck) in 0.1M cacodylate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μL of extract containing total lipids were separated on TLC (Silica Gel 60, Merck) by using petroleum ether:diethyl ether (90:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos from in vitro culture were fixed in a solution containing 2% paraformaldehyde (PFA, Merck) for 20 min at 37° C [41] and ex vivo flushed embryos were fixed in 4% PFA over night at 4° C ...
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bioRxiv - Immunology 2022Quote: Nose and throat swabs were collected in 2 ml transport medium containing 15% sucrose (Merck), 2.5 µg/ml Amphotericin B ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl of terminated reaction mix were spotted onto polyethyl-enimine-cellulose plates (Merck, Germany). ATP and released phosphate were then separated chromatographically in a buffer of 0.5 M LiCl ...
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bioRxiv - Microbiology 2023Quote: ... 300 μL of aqueous phase was added to 500 μL of 2-propanol (Merck, Germany) and kept overnight at −20°C for RNA precipitation ...
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bioRxiv - Microbiology 2022Quote: ... After testing for SARS-CoV-2 by PCR test (COBAS 6800, Merck México, Mexico city), they were classified as positive (AP ...
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bioRxiv - Microbiology 2022Quote: ... Maize was cultured in ¼ Hoagland’s medium (Hoagland’s No. 2 Basal Salt Mixture, Sigma-Aldrich/Merck), pH adjusted to 5.6-5.8 with KOH ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal cavity cells were obtained by lavage with 5 mL PBS + 2 mM EDTA (Merck). Following gentle massage ...
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Microbiology 2023Quote: All compounds (zaprinast, heparin, artemisinin, chloroquine, brefeldin A and Torin 2) were sourced from Merck KGaA ...
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bioRxiv - Biophysics 2023Quote: ... RNA from yeast diluted in PBS (0.7 and 2 mg/mL; Sigma-Aldrich, Merck #10109223001).
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bioRxiv - Developmental Biology 2023Quote: ... rat anti tyrosinated-α-tubulin 1:250 (MAB1864-I, clone YL1/2, EMD Millipore/Merck), mouse anti-Armadillo/β-Catenin 1:50 (N27A1 ...
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bioRxiv - Microbiology 2023Quote: ... 300 µL of aqueous phase was added to 500 µL of 2-propanol (Merck, Germany) and kept overnight at −20°C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 200µM of 1-phenyl-2-thiourea (PTU, ref. n°P7629, Merck/Sigma-Aldrich) to avoid pigmentation ...
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bioRxiv - Bioengineering 2023Quote: ... followed by incubation in 10 mL of 2 U/mL dispase solution (D4693; Merck KGaA) at 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: After saponification with 2 mL 1M 95% ethanolic sodium hydroxide solution (Merck KGaA, Darmstadt, Germany) at 60°C for one hour ...
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bioRxiv - Cancer Biology 2023Quote: ... femurs and tibias in FACS buffer (PBS supplemented with 2% fetal calf serum (FCS; Merck) and 2 mM EDTA (Merck) ...
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bioRxiv - Genetics 2023Quote: ... was added to each along with a single sterile 2 mm glass bead (Merck, Germany) to each tube ...
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bioRxiv - Cancer Biology 2023Quote: Samples were prepared with hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck, Darmstadt, Germany) and loaded onto a 15 % SDS polyacrylamide gel to separate the proteins according to their size ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.12 U/μL RNase Inhibitor) inside a 2 mL Sorenson Dolphin microcentrifuge tube (Merck). The mixture containing the nuclei (800 μL ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto a 2 ml column containing Ni-NTA His·Bind resin (Merck). The column was first washed with a solution containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Microbiology 2023Quote: ... transepithelial electric resistance (TEER) measurements were performed using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01 ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... The final protein preparations were concentrated to 1 mM using Amicon® Ultra 10 or 3 kDa cut off centrifugal filters (Merck KGaA). Aliquots were frozen in liquid nitrogen and stored in −80 °C.
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2021Quote: ... and 10% ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive ...