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Citations for Merck :
2051 - 2100 of 4391 citations for 7 Amino 4 methyl 1 8 naphthyridin 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with 2 ml of pre-warmed 2xSSC with or without RNAseA (50 μg/ml final concentration, Merck) and incubated 1h30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated in standard assay buffer at 37°C and 2 µL aliquots were removed at appropriate time points and spotted on a silica gel 60 F454 plate (Merck). The separation was carried out in butanol:ethanol:milliQ water (5:3:2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse LL/2 (LLC1; ATCC no. CRL-1642; obtained in 2015) was grown in BME with Earle′s salts (Merck) with the same supplementation as mentioned above ...
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bioRxiv - Cell Biology 2021Quote: Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... (+/-)-Abscisic acid (ABA, CAS No:14375-45-2), and Gibberellic acid (GA3, CAS No: 77-06-5) were purchased from Merck KGaA/ Sigma-Aldrich (Darmstadt ...
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2020Quote: ... a five-day-old larva was embedded at the bottom of the central glass compartment in 2% low-gelling temperature agarose (Merck). The agarose was carefully removed around the eyes and mouth upon hardening and the arena was filled with 3 mL water ...
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bioRxiv - Neuroscience 2020Quote: Cell-cycle kinetic differences were assessed by labelling cortical progenitor cells using a nucleotide analog 5-ethynyl-2’-deoxyuridine (EdU; Merck) in vivo following 150 □g EdU injection into the peritoneal cavity of pregnant mice 1 hour before the sacrifice of the embryos ...
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bioRxiv - Cell Biology 2020Quote: 5×106 cells were stained with appropriate antibodies for 20min on ice in PBS + 2% FCS and analyzed on an ImageStreamX MKII imaging flow cytometer (Merck). Mfge8-eGFP+ cells were gated using the IDEAS software ...
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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Revisiting a GWAS peak in Arabidopsis thaliana reveals possible confounding by genetic heterogeneitybioRxiv - Genetics 2021Quote: ... 2 µl of each sample was injected into a SeQuant ZIC-pHILIC HPLC column (Merck, 100 × 2.1 mm; 5 µm), and the respective guard column operated with an Ultimate 3000 HPLC system (Dionex ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed three times in 0.1 M sodium cacodylate buffer pH 7.2 containing 0.2 M sucrose and 2 mM MgCl2 (Merck Millipore Darmstadt, Germany), and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co ...
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bioRxiv - Immunology 2021Quote: ... neuraminidase-specific inhibitor DANA was added to a final concentration of 100 μM (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, Merck, USA). It was used for 48 h prior stimulus.
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and stained with indicated primary antibodies for 2 h at RT followed by incubation with PLA probes (Merck, Duolink® In Situ PLA® Probe Anti-Rabbit PLUS ...
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bioRxiv - Biophysics 2022Quote: ... Buffer was changed to 1Xpbs (Biological Industries, Israel) using multiple washes on Amicon ultra-2 columns (Merck, Burlington, MA #UFC203024).
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bioRxiv - Bioengineering 2022Quote: ... samples were blocked and permeabilized with 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma-Aldrich now Merck) in PBS for 30 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Hydrolysis of 44 soluble non-chromogenic monoester substrates (Table S2) and T-2 mycotoxin (Merck Life Science S.L.U., Madrid, Spain) was assayed at 37ºC using a pH indicator assay with Phenol Red and monitored at 550 nm (46) ...
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bioRxiv - Molecular Biology 2022Quote: ... Fragmented RNA was subjected to two rounds of m6A immunoprecipitation for 2 hours each using an anti-m6A antibody (ABE572, Merck) previously conjugated to protein-A magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old seedlings grown on plates under 16-hour photoperiod using RNAzol reagent (Sigma-Aldricht, Merck) according to the manufacturer’s instructions and DNA precipitation with a 4-fold volume of bromoanisole ...
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bioRxiv - Plant Biology 2022Quote: Fungal elicitor mix was prepared as published previously (24, 38) and separated via enrichment using Discovery DSC-C18 (2 g) columns (Merck) with a H2O/MeOH gradient from 100 % to 0 % H2O in 10 % steps ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissociated follicles were kept on the Transwell-COL membranes contacted with IVG medium composed of αMEM supplemented with 2% polyvinylpyrrolidone (PVP360, Merck), 5% FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Microbiology 2023Quote: ... were pre-prepared by washing 3 times in immunoprecipitation buffer then resuspended in 200μl immunoprecipitation buffer and coated with 2 μl FLAG M2 (Merck Millipore) or IgG (Merck Millipore ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of gDNA from individual GCs (2 ng input) was performed with1 ul of JH reverse primer (10 uM, provided by MERCK) and 1.8 ul of LD forward primer set pools (10 uM per primer ...
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bioRxiv - Pathology 2023Quote: ... To prevent unspecific labelling the sections were incubated for 2 × 10 minutes in PB solution containing 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Biochemistry 2023Quote: 500 ng – 5 µg of pre-purified rRNA subunits (20 µL of each) were digested to single nucleosides by using 2 U benzonase nuclease (> 90 % purity, Merck) 0.2 U phosphodiesterase I (VWR) ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
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bioRxiv - Molecular Biology 2023Quote: ... Target cells without effectors served as a negative control and target cells incubated with 2% Triton X-100 (Merck-Sigma) served as positive control (maximum killing).
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bioRxiv - Biophysics 2023Quote: ... The C378A or C406A variants of 15N-pm-β2AR-Cter were labeled on the remaining cysteine using 3-(2-Iodoacetamido)-proxyl (Merck). Paramagnetic samples were recorded with a recycling delay of 2 s ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were collected and concentrated to ∼2 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All purification steps were performed at 4 °C.
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bioRxiv - Developmental Biology 2023Quote: ... 2-cell stage embryos were similarly recovered from hybrid superovulated females (using 5IU of PMSG and 5IU of hCG; Merck), generated after crossing BDF1 male and CD1 females (each strain obtained from Anlab ...
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bioRxiv - Cell Biology 2023Quote: ... the media of the cells was replaced with XF base media supplemented with 2 mM L-Glutamine (Merck Life science), 17.5 mM glucose (Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Biochemistry 2023Quote: ... containing labeled or unlabeled glucose/glutamine and quenched by adding 50% ice-cold methanol containing 2 µg/mL cinnamic acid (Merck) as an internal control ...
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bioRxiv - Neuroscience 2023Quote: ... A 0.1 M phosphate buffer at pH 7.2 was used and a 2% agarose gel enriched with monodisperse glass spheres (Monospher 100, Merck, Darmstadt) to simulate microscopic B0 inhomogeneities was added ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Genetics 2023Quote: ... neo-synthesized DNA was labeled by two successive pulses (30 min each) with IdU 20 μM (5-iodo-2’-deoxyuridine, 57830, MERCK) and CldU 100 μM (5-chloro-2’-deoxyuridine ...
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bioRxiv - Genomics 2023Quote: ... DNA was fragmented in a 2 mL low-bind round bottom Eppendorf tube using a sterile 3 mm borosilicate bead (Z143928-1EA Merck) by vortexing for 1 minute at maximum speed as described in [23] ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T/phoenix-AMPHO cells were selected for stable expression of retroviral vectors using Hygromycin B (200 µg/mL) and Diphtheria toxin (2 µg/mL; Merck) [16] ...
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bioRxiv - Physiology 2023Quote: ... was used to slowly inject 200 μL of the injection mix composed of ∼2×1011 genome copies of AAV diluted in phosphate-buffered saline (P4417, Merck) containing 2.5 mM MgCl2 and 1 mM KCl ...