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Citations for Merck :
2301 - 2350 of 4391 citations for 7 Amino 4 methyl 1 8 naphthyridin 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was washed once in 5 mL of Honda buffer then purified on a Percoll density gradient as follows: 2 mL of 75% Percoll (Merck, P7828) in Honda buffer topped with 2 mL of 40% Percoll in Honda buffer topped with the nuclei pellet resuspended in Honda buffer in a 15 mL tube ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM MgCl2) containing 1% BSA and incubated overnight with the G4-specific antibody (BG4, MABE917, Merck) at 500 ng/mL in BG4 buffer containing 1% BSA ...
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bioRxiv - Genetics 2019Quote: ... rabbit anti-TbRBP16 [1:1,000] (27) and mouse anti-EF1a [1:200,000] (clone CBP-KK1, Merck-Millipore) for 18 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... in the presence of enzymatic digestion solution containing 1 mg ml-1 collagenase II (Merck Millipore, 234155), 1 mg ml-1collagenase IV (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked for 1 hour in PBS supplemented with 1% Tween-20 (Merck MP Biomedicals) and 5% skimmed milk (Friesland Campina) ...
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bioRxiv - Developmental Biology 2022Quote: ... different concentrations from 1:10 to 1:10000 (V/V) were freshly diluted in absolute ethanol (Merck). For NaCl chemotaxis ...
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bioRxiv - Cell Biology 2022Quote: ... 1:600-diluted rabbit anti-CENP-C (#554),55 mouse anti-HP1α (1:1,000, #MAB3584, Merck Millipore), rabbit anti-HP1β (1:800 ...
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bioRxiv - Microbiology 2023Quote: ... diluted 1 : 100 in 1 × PBS buffer (pH 7.5) with 0.1 % hydrogen peroxide (stock concentration 30 %, Merck) and 2 mM ATP (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Ki67 (rabbit, 1:400, CST, Cat. no 9661S) and cCas3 (rabbit, 1:600, Merck, Cat. no AB9260). The cells were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2024Quote: ... Postfixation was applied using 1% Osmiumtetroxid (Science Services, München, Germany) and 1% Potassium hexacyanoferrat (Merck, Darmstadt, Germany) for 30 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the blots were incubated with primary antibodies α-FLAG (Merck, F1804, 1:1000], α-TPLATE34(1:1000), α-AtEH1/Pan135(1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were allowed to adhere overnight (16 h) in the presence of 2 mM Thymidine (S-phase block; Merck, Darmstadt, Germany) and released into fresh medium ...
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bioRxiv - Microbiology 2019Quote: ... Under these conditions nitrite was not detectable with a colorimetric test with a lower detection limit of 2 mg/L (MQuant test stripes, Merck, Darmstadt, Germany). Growth conditions and operation of the bioreactor containing ANME-2d archaea enriched from an Italian paddy field soil are described by Vaksmaa et al. ...
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bioRxiv - Neuroscience 2021Quote: TEER across cellular monolayers was measured with chopstick electrodes in 24-well Transwell inserts using the Millicell® ERS-2 Voltohmmeter (Merck Millipore). The absolute TEER of eGFP-hCldn5-MDCK II cells before treatment was recorded as a baseline reading ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequent thorough rinsing with ultrapure water (18.2 MΩ at 25 °C, maximum 2 ppb total organic carbon, Milli-Q Integral 5 Water Purification System, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biochemistry 2020Quote: DNA encoding for Pry1 and Na-ASP-2 were PCR amplified and cloned into NcoI and XhoI restriction sites of pET22b vector (Novagen, Merck, Darmstadt, Germany), which contains a pelB signal sequence to direct the secretion of expressed protein into the periplasmic space ...
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bioRxiv - Plant Biology 2021Quote: ... 1000 μl tips were fitted with 2 plugs of C18 octadecyl 47mm Disks 2215 (Empore™) material and 10 μg of LiChroprep® RP-18 peptides (Merck). Tips were sequentially equilibrated with 100 % methanol ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Neuroscience 2022Quote: ... were randomly assigned to receive an injection of 0.9% NaCl v/v 5-bromo-2’Deoxyuridine (BrdU) 100 mg/kg (MERCK; New York, USA) three times within the same day ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The total extract of proteins was then centrifuged for 20 min at 20,000 g and the soluble fraction loaded on an affinity chromatography with 2 ml of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). The resin was washed with 20 ml of 20 mmol/L Tris-HCl (pH7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Microbiology 2021Quote: IFN-α and IFN-β or IL-6 and TNF-α were measured in supernatants of AMs or lung lysates using IFN alpha/IFN beta 2-Plex Mouse ProcartaPlex™ immunoassay (ebiosciences) or Milliplex MAP Mouse™ assay (Merck), respectively ...
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bioRxiv - Genomics 2021Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope slides and frozen in −30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). The frozen liver samples were embedded in cryomolds (10×10 mm ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Plant Biology 2022Quote: ... disposable 200 μl pipette tips were fitted with 2 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1mg:10 μg of LiChroprep® RP-18 (Merck) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were subjected to sonication on ice (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals) followed by incubation for 20 min at 37 °C in the presence of micrococcal nuclease (2 units/sample; Merck, cat. # N3755) and CaCl2 (at 10 mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... (MW 360 kDa) and (Hydroxypropyl)-methylcellulose (HPMC) (viscosity 40-60 cP, 2 % in H2O (20 C) were purchased from Merck (Darmstadt, Germany). Methanol ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with 50 µM inhibitor (dissolved at 50 mM concentration in 100% DMSO) or 2 mM ATPψS (Merck Life Science UK) at room temperature for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Biochemistry 2023Quote: ... The culture was grown for 2 hr at 37°C and stocks were made from the original plate by adding glycerol (Merck & Co., USA) to the final concentration of 15% and stored at -70°C ...
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bioRxiv - Neuroscience 2023Quote: Blood was collected in 2 ml EDTA tubes that were prepared with 100µl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany). These tubes were centrifuged immediately after venepuncture for 15 min at 4⁰C and 3200g ...
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bioRxiv - Biochemistry 2023Quote: ... samples were concentrated to a volume of approximately 2 mL using Amicon® Ultra centrifugal filters with a NMWC of 10 kDa (cat# UFC801024, Merck Millipore).
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bioRxiv - Genetics 2023Quote: ... after adding 2 g activated (i.e., baked at 300°C for 2h) molecular sieves (5Å, 45-60 mesh size, Merck, KGaA, Darmstadt, Germany). The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Neuroscience 2022Quote: ... freezed 15 min at −80°C and thawed for 15 min at room temperature following overnight incubation in 2% TritonX-100 (Merck Millipore, 1086031000) PBS for permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope or Visium slides and frozen in -30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). For spatial experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein complexes were concentrated to 0.7-1 mg ml-1 using an Amicon Ultra-0.5 mL concentrating device (Merck) with a 100 kDa filter cut-off ...
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bioRxiv - Genetics 2019Quote: ... These were then re-solubilized in 30μl chloroform/methanol (1:1) and loaded onto silica gel plates (Merck). PA and DAG were separated by 10-15min of thin layer chromatography (TLC ...
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bioRxiv - Microbiology 2019Quote: ... Antibody staining was performed using EV-A71 monoclonal antibody MAB979 (Merck, USA; 1:2500 dilution in 1 % BSA) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM EDTA and 1 mM DTT and concentrated with Amicon Ultra-0.5 mL centrifugal filter (Merck #UFC503096). Nucleosomes were stored in 4 °C and the quality of nucleosomes was assessed by 5-6 % TBE gel.
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:1 dilution with tris-buffered saline (TBS) containing 0.1% (v/v) Tween® 20 (Merck). Subsequently ...
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bioRxiv - Biophysics 2022Quote: ... All pH adjustments were performed using 1 M HCl or 1 M NaOH (99.0 %, Merck KGaA, Darmstadt, Germany). All samples were filtered prior to stress application through a 0.1 µm inorganic membrane filter (#6809-2012 ...
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bioRxiv - Immunology 2020Quote: ... rAAVDJ or rAAV6 genome plasmid and Donor plasmid at a 3:1:1 ratio in Polyethylenimine (PEI)(Merck). In total each plate was teransfected with 41,250ng of DNA ...
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bioRxiv - Microbiology 2022Quote: ... the samples were mixed vigorously at a ratio of 1:1 with saturated phenol solution pH 4.3 (Merck) and centrifuged for 15 min at 13’000 rpm ...
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bioRxiv - Plant Biology 2019Quote: ... 0.8 % phytoagar (Duchefa) and 10 µg mL-1 Glufosinate-ammonium or 25 µg mL-1 hygromycin B (Merck) for herbicide selection ...