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Citations for Merck :
2001 - 2050 of 2489 citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing BirA were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Biophysics 2024Quote: ... The isolated protein was concentrated using Amicon® Ultra centrifugal filter with a molecular cutoff 10 kDa (Amicon Ultra-4 Centrifugal filter unit 10K, Merck). The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250 ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Remaining chromatin extracts were incubated overnight at 4°C with 50 µL of prewashed anti-Flag M2 magnetic beads suspension (Millipore, Merck M8823) per sample ...
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bioRxiv - Plant Biology 2024Quote: ... 6xHis:SidK:3xFLAG or 6xHis:SidKF62A:3xFLAG protein were added to 1 ml of filtered extract to a final concentration of ∼2.5 μM and incubated in a rotatory mixer at 4°C with 25 μl of Anti-FLAG® M2 Magnetic Beads (Merck). 30 μl of the mixture was taken as input for Western blot analysis before adding magnetic beads ...
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies (synaptophysin Cat#MAB329, Merck Millipore; GluA2 Cat#13607, Cell Signaling; Psd95 Cat#MAB1598, Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... Elution sample was concentrated at 3,300 rpm for 5 minutes per round until it reached 500 uL with Amicon Ultra-4 30K centrifugal filter system (Merck Millipore). Size exclusion chromatography was performed with Superdex200 increase 10/300 column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Pan-Biotech) while the other retina was incubated for 4 h in the same medium supplemented with 100 µM leupeptin (Merck, L2884) and 20 mM NH4Cl (Merck).
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bioRxiv - Immunology 2024Quote: ... ankle tissues were soaked in 4% (v/v) paraformaldehyde phosphate buffer solution (Fujifilm Wako) overnight and then replaced with OSTEOSOFT (Merck Millipore) every three days over 11 days for decalcification ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was then collected and filtered with a 3 kDa filter (Amicon Ultra-4 centrifugal Filter 3 kDa MWCO, Merck UFC8003243). Five distinct preparations of ACS <3kDa were prepared from five astrocytes cultures and were then sent to the Protein Analysis Facility of the University of Lausanne for Mass Spectrometry analysis.
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... The dried lipid A samples were resuspended in 30 μl of chloroform/methanol mixture (4:1) for thin-layer chromatography (TLC) analysis using Silica Gel 60 F254 TLC plate (Merck Millipore) with the solvent system consisting of chloroform/pyridine/96% formic acid/water (50/50/14.6/4.6) ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL) for 10 minutes, followed by base piranha cleaning (1:1:4 H2O2 (000855032300, Bio-Lab, IL):NH3 (105432, Merck, DE):H2O ...
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bioRxiv - Plant Biology 2020Quote: ... 0.1 g of the ground material was digested overnight at 90°C in a mixture of 2 ml HNO3 and 0.5 ml H2O2 (30% Suprapur, 1.07298.1000 Merck). Then 50 μl of HF (40% reagent grade ...
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bioRxiv - Cell Biology 2021Quote: ... The Bin1b-SH3 construct was transformed into Eschericia coli RosettaTM 2 (DE3) competent cells (Novagen, Merck, 71403) by heat shock ...
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bioRxiv - Biophysics 2021Quote: ... for 1 min in O2 plasma and incubating them with fibronectin (2 μg/mL) (Merck, Darmstadt, Germany) in 100 mM NaHCO3 (pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and cultured in StemMACS iPS-Brew XF medium (Miltenyi Biotech) supplemented with 2 μM Thiazovivin (Merck Millipore), for 24 h after passaging with daily medium change ...
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bioRxiv - Biochemistry 2020Quote: ... Platelet aggregation was elicited with 2 µM of γ-thrombin (Merck, Watford, UK, final volume 200 µL). Absorbance was monitored at 430 nm every 55 s for 35 min using an Optima plate-reader (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: Intracellular Ca2+ levels were assessed in hippocampal neurons loaded with Fura-2/AM dye (Merck, Darmstadt, Germany) as previously described (Joshi ...
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bioRxiv - Cell Biology 2021Quote: ... vigorously vortexed for 1 min and leukocytes retrieved by centrifugation were fixed using 2 % paraformaldehyde (Merck #158127) on ice for 10 min and washed with PBS-0.1 % sodium azide solution.
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bioRxiv - Immunology 2022Quote: ... The eluted fractions were cleaned and concentrated in Amicon Ultra-2 30 kDa (Merck Millipore, cat: UFC203024) by diluting them in PBS (150 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... Phophorylated MEK was detected using anti-phospho MEK 1/2 (residues 218/222 and 222/226, MERCK, catalogue No ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed another 2 times and permeabilised with 0.1% Triton™ X-100 (Merck, UK) diluted in PBS and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Physiology 2022Quote: ... was used to identify the vasa recta capillaries and anti-neural-glial 2 (NG2; AB5320, Merck-Millipore) was used as a marker of pericytes ...
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bioRxiv - Cancer Biology 2022Quote: ... Resistant cells were sorted after TROP2 induction with DOX (final concentration 2 µg/ml; purchased from Merck) by surface labeling with TROP2-specific antibodies (see further).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... excess media was removed from wells and mf were incubated with 0.5 mg/ml MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (Merck) in PBS at 37°C for 90 min ...
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bioRxiv - Immunology 2020Quote: ... and the supernatant was taken for immunoprecipitation with 2 μg of anti-ubiquitin antibody (FK2, Merck-Millipore) for 12 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the cells at a density of 5×105 /ml were supplemented with 2% dimethyl sulfoxide (DMSO; Merck) and the culturing was continued for another 24 to 72 hr ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... dried tissue samples (in liquid nitrogen) were dissolved in 3:2:1 mixture of HNO3 (Merck, Germany), H2SO4 (Merck ...
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bioRxiv - Immunology 2023Quote: ... were premixed at a 1:9 molar ratio or a 2:98 molar ratio in chloroform (Merck) to arrive at PLBs harboring Ni-DOGS-NTA at 10% or 2% ...
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bioRxiv - Neuroscience 2023Quote: ... at 1/1000 dilution or Laurdan (#40227, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, Merck, Sigma-Aldrich) at 1/500 dilution for 30 min at 37°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM EE-analog in 150 µL of CH3OH were incubated with 2 mg/mL NaBH4 (Merck) in 150 µL of CH3OH at 37 °C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... and 0.43 mM MgCl2) with the addition of 0.2 mM 1-phenyl-2-thiourea (PTU, Merck, Germany) to block pigmentation ...
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bioRxiv - Bioengineering 2022Quote: ... Five μL of the sample were injected into a C18 column (Merck Spherisorb ODS-2 (5 μm), 250×4 mm ...
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bioRxiv - Cell Biology 2023Quote: ... Lipids were dissolved in chloroform:methanol (2:1, v:v) and separated by TLC on silica gel 60 (Merck) along with a standard ...
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bioRxiv - Molecular Biology 2024Quote: ... coding sequences of AtCFI25a and AtCFI59 were cloned into pGEX5X-2 (GST) (Merck/GE Healthcare, Darmstadt, Germany) or pET30c (HIS ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 heads per sample were homogenised in 100 μL 2% SDS buffer (Cat No. 428018, Merck) containing 1X RIPA buffer (Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... in phosphate-buffered saline (PBS) and subsequently blocked in a solution containing 2% normal goat serum (Merck) within the same permeabilization solution ...
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bioRxiv - Plant Biology 2023Quote: ... pericarp sections were incubated for 2 h in a 5% (v/v) Normal Donkey serum (NDS; Merck) blocking solution in MTSB ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was then incubated with 2 mL pre-washed HIS-Select Cobalt Affinity Gel (Merck, UK) with rotation for an hour ...
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bioRxiv - Bioengineering 2022Quote: ... The nanobody was reduced using 10 mM Tris(2-carboxyethyl)phosphin -hydrochlorid (TCEP, #51805-45-9, Merck) on ice for 1h ...