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Citations for Merck :
1901 - 1950 of 2489 citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Bioengineering 2021Quote: ... with Pellicon 2 mini cassettes (300 kDa, 0.1 m2, BioMax polyethersulfone membrane with C-screen; Merck), as previously described (4) ...
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bioRxiv - Biophysics 2021Quote: ... Cells at 2-3 x 106 cells/mL were induced with 50 ng/mL doxycycline (Merck) and 5 mM valproic acid (Cayman Chemical) ...
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bioRxiv - Cancer Biology 2020Quote: ... media was replaced with media containing 10 nM 5-Bromo-2′-deoxyuridine (BrdU) (Merck, B5002-100MG). Coverslips were then fixed ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated at a density of 100,000cm-2 in 96 well plates (Corning, MERCK UK), with wells previously coated for 24h with 20μg/ml FHL-1 ...
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bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37°C and purified using a Amicon 30 kDa MWCO filter (Merck). Deep Vent exo-DNA polymerase (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... cells were 12 x serially diluted 1:2 in a 96-well plate (Merck, Darmstadt, Germany) starting with 4000 cells in the first column ...
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bioRxiv - Immunology 2020Quote: ... 2-3 × 105 cells/well were plated in an ELI Spot plate (MAHAS4510, Merck Millipore, USA) and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or ...
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bioRxiv - Biochemistry 2020Quote: ... then supernatant concentrated to 2 mL using 3 kDa Amicon Ultra-15 Centrifugal Filter Units (Merck). Supernatant was then washed 3 times with 15 mL 10 mM ammonium acetate to remove residual erythromycin ...
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bioRxiv - Immunology 2021Quote: ... The left lung was inflated with 0.6mL PBS/2% (w/v) low melting point agarose (Merck) in PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in serum-free MEM supplemented with 2 µg/mL TPCK-treated trypsin (Merck) for 2 days at 37 °C in 5 % CO2 ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 10×106/100μL in PBS containing 2% FCS (Merck). Staining for cell surface antigens was performed for 20 minutes at 4°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Non-adherent cell culture plates were prepared with poly(2-hydroxyethyl methacrylate) (poly-HEMA; P3932, Merck) coating ...
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bioRxiv - Microbiology 2023Quote: ... One mL of culture broth was mixed in a 2:1 ratio with chloroform (Merck, Mumbai), followed by centrifugation (15,300 g ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3-(3,5-dioxo-1,2,4-oxadiazolidin-2-yl)-L-alanine (quisqualate) was purchased from Merck (Darmstadt, Germany). AF647-conjugated 9E10 antibody was prepared in-house as described previously (Cook et al. ...
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bioRxiv - Microbiology 2022Quote: ... and half of the water was replaced with freshwater (2/3 osmosis (RiOs 5, Merck Millipore) and 1/3 filtered ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified cells were cultured into organoids and treated with 2 μg/ml doxycycline (DOX, Merck, D9891) and 10 μM trimethoprim (TMP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human Caco-2 colorectal adenocarcinoma cells (1.7×105 cells/cm2) were cultured in DMEM medium (Merck) containing 10% FCS at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Finally, clearing was performed in BABB (benzyl alcohol:benzyl benzoate 1:2, (Merck 8187011000 and Sigma 108006) for 48h.
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bioRxiv - Physiology 2023Quote: ... an amount of 2 g/kg body weight D-glucose (20% solution in PBS+/+, Merck, USA) was injected ...
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bioRxiv - Biophysics 2023Quote: ... 2 mL of supernatant containing viral particles was harvested and filtered with 0.45 μm filter (Merck). Supernatant was immediately used for transduction or stored at -80°C in aliquots.
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bioRxiv - Microbiology 2024Quote: One mL of culture broth was mixed in a 2:1 ratio with chloroform (Merck, Mumbai), followed by centrifugation (15,300 g ...
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bioRxiv - Bioengineering 2022Quote: ... and LX-2 cells were labeled with a PKH67 Green Fluorescent Cell Linker Kit (Merck, PKH67GL) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... in 30% (2-Hydroxypropyl)-β-cyclodextrin (HPβCD) or Sulfobutylether-β-Cyclodextrin (SBEβCD) (Sigma Aldrich, Merck, Germany). When tumors reached a size of 60-100 mm3 ...
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bioRxiv - Developmental Biology 2022Quote: ... During day 13 to 15 they were treated with 2 pulses of 3μM CHIR99021 (Merck, 361571) to dorsalise the tissue ...
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bioRxiv - Developmental Biology 2023Quote: ... 25 μl of 2 mg/ml of 5ʹ-nucleotidase (snake venom from Crotalus atrox; Merck KGaA) in 0.1 M Tris-HCl pH 8.0 were added ...
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bioRxiv - Physiology 2024Quote: ... The supernatant was filtered through glass wool and mixed with 2 liters of DEAE-sepharose (Merck) pre-equilibrated with Buffer A+ ...
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bioRxiv - Neuroscience 2024Quote: ... the neuronal microtubule cytoskeleton was labeled using rabbit anti-MAP-2 (Merck Millipore, #AB5622, 1:800). Following three washes ...
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bioRxiv - Bioengineering 2024Quote: ... followed by 10 min of crosslinking in a 2% (w/v) calcium chloride (CaCl2; Merck, Germany) solution at RT ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured with 50 U/ml of rhIL-2 in RPPMI-1640 medium (#8758, Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone ...
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bioRxiv - Cancer Biology 2024Quote: ... cells have been lysed using 2× Laemmli buffer supplied with 100mM DTT (Dithiothreitol, Merck Sigma Aldrich), boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico ...
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bioRxiv - Molecular Biology 2024Quote: pCold-DDX43 and pCold-DDX6 were transformed into Rosetta 2 (DE3) competent cells (Merck Millipore/Novagen). The cells were cultured at 37°C until the OD600 reached ∼0.6 ...
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bioRxiv - Biophysics 2024Quote: ... followed by 1 hour incubation with 2 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich/Merck, #C4555).
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells (1.2 x106) were synchronized by incubating with 2 mM thymidine (T1895, Merck, Darmstadt, Germany) for 24 hours ...
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Enabling Epitope Analysis of Glycoproteins via HDX-MS Using Antigens with Uniformed N-linked GlycansbioRxiv - Biochemistry 2024Quote: ... The eluates were concentrated using an Amicon Ultra-2 Centrifugal Filter Unit 30kDa NWMCO (Merck Millipore), in which the elution buffer was replaced with PBS (GIBCO).
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bioRxiv - Cell Biology 2020Quote: ... Stag-Tulp3 was then pulled down from the TEV digestion eluates by overnight 4°C rotational incubation using S-protein agarose beads (Merck, #69704). Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... Mouse peritoneal macrophages (MPMs) were isolated from mice 4 d after the intraperitoneal injection of thioglycollate (Merck & Co.; Kenilworth, NJ, USA) as described previously(Zhang et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... the following antibodies were treated to the organoids and the tissue slices at 4°C overnight: proSFTPC (1:200; Merck, AB3786), E-cadherin (1 ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Immunology 2021Quote: ... Histone neutralisation experiments were performed via intraperitoneal injection with dialysed and combined a-Histone 3 and a-Histone 4 antibodies (Merck Millipore) or control polyclonal rabbit IgG (BioXCell) ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C with a SW28 rotor in a Beckman XL-70 centrifuge or concentrated using an Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore). Pellets were resuspended in 500 µL PBS ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing monomeric I-Ek/MCC(ANP)-biotin complexes were concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK), snap frozen in liquid nitrogen and stored in 1x PBS at −80 °C ...
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bioRxiv - Biophysics 2020Quote: ... dialyzed against Milli-Q water and concentrated to 4 mg ml-1 using Amicon Ultra centrifugal filter units with Ultracel-50K (Merck Millipore). UMODe filaments were obtained by limited digestion of UMODfl with elastase (Jovine et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... a blocker of the sarco/endoplasmic reticulum Ca2+-ATPase pump (SERCA) and Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP, Merck-Sigma-Aldrich), a protonophoric uncoupler of mitochondrial oxidative phosphorylation that depolarizes the mitochondrial membrane and leads to the organelle’s release of calcium ...
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bioRxiv - Cell Biology 2021Quote: ... 25 min) and alkylated (20 mM iodoacetamide, 30 min, room temperature) and samples concentrated with Amicon Ultra-4 columns (Merck-Millipore), After protein precipitation with methanol/chloroform ...
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bioRxiv - Microbiology 2020Quote: Feces were hydrated in 500 µL sterile Milli-Q water overnight at 4 °C and then mixed with 500 µL of absolute ethanol (Merck, USA) for 60 min at RT ...
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bioRxiv - Plant Biology 2020Quote: ... on a LiChroCART® column (LiChrospher 100 RP-18, 125 x 4 mm, 5.0 μm particle size, Merck KGaA, Darmstadt, Germany), with constant flow rate of 1 mL min-1 of 100% solvent A (acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... To perform this, tissues were fixed over a filter paper imbibed with 30% sucrose (Winkler, Chile) in PBS–4% paraformaldehyde (Merck, USA) for at least 15 min ...
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bioRxiv - Plant Biology 2021Quote: ... Individual carotenoids were quantified in a Shimadzu HPLC (LC-10AT) with a diode array detector using a RP-18 Lichrocart 125-4 reverse phase column (Merck®) and a mobile phase composed of acetonitrile:methanol:isopropanol (85:10:5 v/v) ...