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Citations for Merck :
151 - 200 of 487 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: The samples were lysed in RIPA buffer (50 mM Tris (Merck, pH = 8), 150 mM NaCl (Merck) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were stimulated with IFN-β (1,000 U/ml, 8 h, Merck), IFN-a2 (500 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Released glycans were fluorescently labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS, Merck). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... in the b-galactosidase tissue stain base solution (BG-8-C, Merck-Millipore) with either X-gal (B4252 ...
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bioRxiv - Microbiology 2023Quote: ... Transductions were performed in the presence of 8 μg/mL polybrene (Merck Millipore). Virus supernatant was removed at 8 hr post-transduction and cells were maintained in fresh growth medium for 48 hr before undergoing 3 µg/mL puromycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells in a Nunc® Lab-Tek® 8-well chamber slide (Merck) were washed with Phosphate Buffer (PB ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Biophysics 2021Quote: ... Human pancreatic beta cells 1.4E7 were purchased from Merck and cultured in RPMI-1640 medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... conidia were opsonized with normal human serum (Merck Millipore). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... human mitochondria (clone 113-1, Merck Millipore, 1:100), and BrdU (clone BU1/75 ICR1 ...
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml human EGF (Merck, Cat Number: E9644), 0.5 mg/ml hydrocortisone (Merck ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg/mL mouse anti-human TGN46 (SAB4200355, Merck), or a 1 in 20 dilution of rat anti-β-tubulin (clone YL1/2 ...
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bioRxiv - Biophysics 2021Quote: Rabbit polyclonal anti human IRSp53 (07-786 – Merck Millipore), mouse monoclonal anti CA (24.2 – NIH AIDS Reagent Program – FisherBioServices) ...
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bioRxiv - Biophysics 2021Quote: ... rabbit polyclonal anti human IRSp53 (07-786 – Merck Millipore), mouse anti CA (183H125C – NIH3537) ...
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bioRxiv - Molecular Biology 2021Quote: Caco-2 cell line human colon adenocarcinoma (Merck, UK) were cultivated in DMEM (Sigma ...
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bioRxiv - Bioengineering 2021Quote: The human immortal chondrocyte cell line – C28/I2 (Merck) was used in this study ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 mg/mL Human recombinant Insulin (Merck, Cat. #91077C) and 1% Pen/Strep ...
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.2 IU/ml human chorionic gonadotropin (HCG) (C1063, Merck), 25 µg/ml sodium pyruvate and penicillin-streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg/ml of IgG from human serum (Merck) in PBS were added to all blocking and antibody incubations ...
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bioRxiv - Cell Biology 2023Quote: ... were coated with human plasma fibronectin (Merck Millipore FC010) at a concentration of 5 µg/cm2 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 IU/ml recombinant human FSH (Merck & Co., Inc), 0.125 mg/ml recombinant human hyaluronan (Novozymes) ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated goat anti-human IgG (AP309P, Merck KGaA). For stripping and re-probing of Western blot membranes ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore), and anti-E-cadherin-1 (ECAD ...
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bioRxiv - Microbiology 2024Quote: ... the bacterial suspension and human serum (Merck, Darmstadt, Germany) were mixed in a 1:1 ratio (100 µL each) ...
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bioRxiv - Cell Biology 2024Quote: ... 50nM Mission esiRNA against human PTGER2 or PTGER4 (Merck). 50nM non-targeting Silencer™ Negative Control #1 siRNA (Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 concentrations were determined with MILLIPLEX MAP Porcine Cytokine/Chemokine Magnetic Bead Panel (Merck-Millipore) with strict adherence to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Cell Biology 2021Quote: ... Culture dishes and coverslips were coated with 0.1% gelatin (CAS 9000-70-8, Merck) in phosphate-buffered saline 30 min prior to cell seeding ...
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bioRxiv - Neuroscience 2020Quote: ... using Essential 8 Flex medium supplemented with 10 μM ROCK inhibitor (Y-27632; Merck). At differentiation day 0 ...
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bioRxiv - Genomics 2019Quote: ... The cells were incubated with an antibody against 8-oxoG (Merck KGaA, Darmstadt, Germany) diluted 1:200 in 0.05% Tween 20 in PBS for overnight at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: The homogenization with 8 U/ml proteinase K (PK, #P4850 Sigma-Aldrich now Merck) in digestion buffer (50 mM TRIS (#AE15.2 ...
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bioRxiv - Physiology 2023Quote: ... cells or tissues were lysed in RIPA buffer (50 mM Tris pH 8 (Merck),150 mM NaCl (Merck) ...
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bioRxiv - Neuroscience 2024Quote: Epoxiconazole (PESTANAL®, analytical standard, racemate) (CAS 133855-98-8) was acquired from Merck® (Darmstadt ...