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Citations for Merck :
1 - 50 of 487 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 and NIH-3T3 cells were cultured in DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Merck), JQ-1 (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... whereas HEK293 cells were cultured in DMEM (D5796 MERCK, Darmstadt, Germany) containing 10% (vol./vol. ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Merck) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... 8) eosin (Merck. #1.15935.0100) for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mM MgCl2 (Merck), 40 mM EGTA (Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mM MgCl2 (Merck), 40 mM EGTA (Sigma)) ...
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bioRxiv - Cell Biology 2023Quote: ... CCK-8 (96992, Merck) was used according to the manufacturer’s instructions and measurements were performed at the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... 8 mM MgCl2 (Merck), 320 mM sucrose ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Neuroscience 2024Quote: ... we used fixed HEK293 cells labeled with primary antibody (rabbit anti-GM130, 1:1000, Merck) and secondary antibody (goat anti-rabbit conjugated with Alexa Fluor 555 ...
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bioRxiv - Immunology 2022Quote: ... IL-6 and IL-1RA was performed using the Luminex color-coded antibody-immobilized beads from Merck Millipore following the manufacture’s indications ...
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bioRxiv - Cell Biology 2022Quote: ... JNK-I-8 (Merck SML1246), SB-203580 (MedKoo ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-8-oxoguanine (MAB3560, Merck), anti-phospho-histone H2AX (Ser 139 ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... BH4 (Merck, 69056-38-8). RPMI SILAC - medium deficient in Arginine was obtained from (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 µL proteinase K (10 mg/mL) and 8 µL of 1 M DTT (Merck) was added to the sperm fraction which were vortexed and incubated at 56 °C for 60 min (mock casework samples ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... 50 mM Tris pH 8 (Merck), 1 % IGEPAL® CA-630 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM Tris pH 8 (Merck), 1 % v/v IGEPAL CA-630 (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μg/ml RNase A (Merck) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... cell counting kit 8 (Merck, 96992) was applied for 2.5 h as recommended by the manufacturer.
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells (8 × 104) were seeded on fibronectin (bovine, 0.1 mg/mL) coated Millicell EZ SLIDE 8-Well (Merck) in 300 µL media/well (RPMI + 10% FCS + 1% ultraglutamine + 1% Pen/Strep + 1% HEPES + 1% Na-pyruvate + 1% MEM NEAA + 0.1% mercaptoethanol).
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bioRxiv - Physiology 2023Quote: ... phloretin (50 mg/L; n=8) or ritonavir (60 mg/L; n=8) (all from Merck, Darmstadt, Germany) in 20 mL of 2.3 % glucose peritoneal dialysis fluid (Balance ...
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: hTERT RPE-1 and HEK293(T) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM/F12; Merck) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... 8 μg/mL Calcein AM (Merck, Germany), 4 μg/mL PI (Merck ...