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Citations for Merck :
151 - 200 of 214 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the sections were treated with 10% methanol in PB for 20 min and then incubated for 1 h at RT in incubation buffer: 5% normal donkey serum (NDS: Merck) and 0.2% Triton X-100 (Merck ...
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bioRxiv - Microbiology 2022Quote: ... Discs were aseptically removed after 48 h and dipped briefly in sterile PBS (Sigma-Aldrich, Merck Lifesciences Ltd, Dorset, UK), to remove any planktonic bacteria after which they were placed into 3 mL of one of the test agents ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Plant Biology 2024Quote: ... digestion of roots was performed at 90°C during 4 h in 400 µL of 65%(w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.65% (w/v ...
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck), 100 µM jasplakinolide (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were incubated for 1 h at RT with a mouse anti-2A primary antibody (cat. no. MABS2005, Merck) diluted 1:2000 in 1% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Media containing high titre pseudo virus was collected at 4 h intervals after the first media exchange and continuously for 48 h thereafter and filtered through a 0.45 μm PES filter (Merck, SLHP033RS) to remove cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant of the transfected cells was collected 48 h later and filtered through a 0.45 μm pore-size filter (Merck Millipore). For viral infection ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were harvested after at least 24 h of incubation at 32°C and filtered on a 0.45 µm filter (Merck #SE2M230I04).
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bioRxiv - Biochemistry 2024Quote: ... Isolation of ilicicolin H from the medium was conducted using the Supelclean™ LC-18 SPE Tubes (Merck, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... the plates were incubated for 1 h at 25°C with peroxidase-conjugated donkey anti-chicken IgY (Merck, NJ, USA) diluted 5000 times with HEPES-EDTA containing 1% (w/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... Stimulation of the controls was performed 8 h after transfection with 20 ng/ml TNF-α (Merck, cat. no.: H8916). Cells were lysed 24 h later by addition of 100 μl lysis buffer (25 mM Tris/HCl ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with blocking solution for 1 h and then with mouse monoclonal anti-NeuN or SMI 32 antibody (1:200; Merck Millipore ...
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bioRxiv - Genomics 2024Quote: ... 20 mL of pre-heated (65°C) modified Cetyltrimethylammonium bromide (CTAB) extraction buffer [2% CTAB (H-5882, Merck, Darmstadt, Gerrmany); 100 mM Tris-HCl (pH8.0) ...
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bioRxiv - Neuroscience 2024Quote: ... blocked for 2 h in blocking solution (5% normal goat serum from Vector Laboratories, 0.5% Triton X-100 from Merck in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... followed by signal development for 6–24 h in a solution containing nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Merck). The samples were covered with glass coverslips using CC/Mount (Merck) ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μL of sample (total homogenate or HEPES soluble fraction) was lyophilized for 12 h and digested with 50 µL of Nitric Acid (HNO3) (65% Suprapur, Merck) overnight at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... cryoprotected for 48 h in 20% sucrose at 4°C and then snap frozen in isopentane (2-methylbutane, Merck GmbH, Austria) for 3 min at −60°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... neurons were stained with 1 μg/ml HOECHST-33342 (H-33342) and 1 μM calcein-AM (both from Merck, Darmstadt, Germany) one hour prior to the imaging ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... the kinetics of ROS production was evaluated for 2 h after the addition of 2,7-dichloro-diidrofluorescineacetate (DCFH-DA, Merck, Darmstadt, Germany) using the Microplate Reader GloMax fluorimeter (Promega Corporation ...
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bioRxiv - Zoology 2023Quote: ... it was fractionated into the different fatty acid classes over an activated silicic acid column (heated at 120ºC for 2 h; Merck Kieselgel 60) via eluting with 7 ml chloroform ...
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bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were freshly grown for 20 to 24 h on plates in anaerobic jars supplemented with Anaerocult C sachets (Merck, USA) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... in T-BST for 1 h at room temperature or Re-Blot Plus Strong Antibody Stripping solution for 15 min at room temperature (Merck, #2504). Quantification of protein bands was performed using ImageJ analysis software (release 1.53c).
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bioRxiv - Microbiology 2024Quote: ... Pan-Biotech) while the other retina was incubated for 4 h in the same medium supplemented with 100 µM leupeptin (Merck, L2884) and 20 mM NH4Cl (Merck).
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bioRxiv - Cell Biology 2024Quote: ... The cells were collected by scratching the plates 72 h post-transfection and filtered using an Amicon Ultra-0.5 centrifugal filter (Merck Millipore, UFC510024). Extraviral DNA was removed by digestion with DNase I (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... PBS rehydrated tissue was then blocked using 10% normal horse serum in 0.2% Triton X-100 in PBS for ∼1 h and then primary antibodies specific for S100 (Merck, S2532, 1:200) TUJ1 (Synaptic systems ...
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bioRxiv - Bioengineering 2021Quote: Inflammatory stimulations were performed by treating the chips for 24 h from d4-d5 or d11-d12 with TNF-α (final concentration of 20 ng/mL; SRP3177; Merck KGaA) or LPS (final concentration of 100 ng/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
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bioRxiv - Neuroscience 2022Quote: ... ileum and colon sections were deparaffinated in histolene and rehydrated in a descending series of ethanol (100%, 96%, 70%) followed by hematoxylin solution (H&E, Merck, Darmstadt, Germany) for 5 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... GSL in 1 ml crude extracts were bound on 250 µl DEAE-Sephadex A-25 columns and digested on the column for 18 h at room temperature with 18U sulfatase H1 enzyme (Merck KGaA, Germany). Desulfoglucosinolates were eluted twice with 1 ml ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...