Labshake search
Citations for Merck :
101 - 150 of 214 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and fixed for at least 3 h with 70% ethanol (Merck, 100983). Cells were washed with PBS and analyzed by using the Cell Cycle Kit (Luminex Corporation ...
-
bioRxiv - Cancer Biology 2023Quote: ... pH 7.9) on ice for 1 h in the presence of EDTA-free Protease Inhibitor Cocktail (Merck) and cleared by centrifugation at 20,000 g for 15 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... pH 7.9) on ice for 1 h in the presence of EDTA-free Protease Inhibitor Cocktail (Merck). Lysates were cleared by centrifugation at 20,000 g for 15 min ...
-
bioRxiv - Plant Biology 2023Quote: ... pericarp sections were incubated for 2 h in a 5% (v/v) Normal Donkey serum (NDS; Merck) blocking solution in MTSB ...
-
bioRxiv - Immunology 2024Quote: ... for 24 h and then used for a time-course of release by adding biotin (#4698, Merck) at a final concentration of 40 μM as previously described (Capitani et al. ...
-
bioRxiv - Molecular Biology 2021Quote: Transfected HEK293T cells were treated for 16 h with DMSO or with 10 µM Cyclopiazonic acid (CPA, Merck) before collection.
-
bioRxiv - Cell Biology 2021Quote: ... After 24 h cells were stained for 30 min at 37 °C with NAO dye (200 nM, Merck), washed ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The H and E-stained sperms on the slides were mounted using Dibutylphthalate Polystyrene Xylene (DPX) (Merck, Germany) and dried overnight at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: Total protein was extracted from the cells 48 h post-transfection by complete lysis-M buffer (Merck, USA). Twenty-five mg of total protein were loaded per well in a 12% SDS PAGE ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteasome complexes were subsequently blotted for 1 h at 200 V on ice onto PVDF membranes (Merck Millipore) and further handled as described above.
-
bioRxiv - Microbiology 2021Quote: ... Mycelia cultured for 24 h in CD medium were collected by filtering through Miracloth (Merck Millipore, Darmstadt, Germany), washed with water ...
-
bioRxiv - Microbiology 2021Quote: ... and infected with pseudovirus for 24 h in the presence of polybrene (10 μg/ml, Merck, Darmstadt, Germany). BMMs were washed with PBS for three times at 24 h after infection ...
-
bioRxiv - Cell Biology 2023Quote: ... After 23 h the cells were detached and reseeded on glass cover slips coated with Concanavalin A (Merck, Darmstadt ...
-
bioRxiv - Developmental Biology 2024Quote: ... sections were mounted with Vecta-Shield (Vector #H-1000) or Mowiol (Roth #0713.2) optionally containing DAPI (Merck #1.24653).
-
bioRxiv - Immunology 2024Quote: ... plates were washed in PBS/T and incubated for 1 h with goat anti-mouse IgG-AP (Merck). Plates were then developed as per the mAb ELISA ...
-
bioRxiv - Neuroscience 2023Quote: ... cryosections were incubated at RT for 1 h in darkness with the secondary antibody and Hoechst 33258 (Merck) as a nuclear counterstain ...
-
bioRxiv - Cell Biology 2022Quote: E10.5 embryos were fixed in 4% PFA/PBS for 1.5 h followed by incubation in 15% sucrose (Merck)/PBS for 2 h and overnight incubation in 30% sucrose/PBS ...
-
bioRxiv - Microbiology 2022Quote: ... An aliquot of 5 μl was spotted on an activated (100 °C, 1 h) TLC sheet (Merck, USA). CM2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Following saturation for 1 h in PBS 1X containing 5% milk and 0.1% Tween 20 (655204-100ML, Merck), the membranes were incubated overnight at 4 °C with primary antibodies ...
-
bioRxiv - Immunology 2024Quote: ... Tissue was stained for 1 h with a mixture of Hoechst-34580 DNA dye (10 µg/mL; Merck) and Phalloidin Alexa 647 conjugate (1:400 ...
-
bioRxiv - Immunology 2024Quote: ... before staining on ice in the dark for 1 h with mouse monoclonal anti-Puromycin-AF647 (Merck Millipore), diluted in the same solution ...
-
bioRxiv - Microbiology 2024Quote: ... E607063-0100) for 2 h to kill non-adherent bacteria and then lysed with 0.1% saponin (Merck; 47036). Lysates were plated on THA plates after serial dilution in PBS and bacterial numbers were counted after overnight incubation ...
-
bioRxiv - Biochemistry 2024Quote: ... with the additional step that lysis was preceded by 4 h treatment with 10 μM MG132 (Merck, 4693159001) and 10 μM PR619 (Bio-Techne ...
-
bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
-
bioRxiv - Biophysics 2021Quote: ... The culture supernatant was collected after 72 h and concentrated using Amicon Ultra-15 MWCO 100 kDa (Merck Millipore).
-
bioRxiv - Cell Biology 2021Quote: ... approximately 1x 106 cells of each cell line were spin-infected for 1 h at 1.9 × 103 g (3000 rpm) in the presence of 10 μg/mL polybrene (Merck) to facilitate the infection ...
-
bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were stored for 24 h at 4°C in 4% PFA and then washed and stored in PBS containing 0.05% sodium-azide (Merck). Within a week of perfusion ...
-
bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
-
bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
-
bioRxiv - Neuroscience 2024Quote: ... qPCR or EdU proliferation assay) for 72 h in the presence or absence of 200 nM Smoothened agonist (Merck). Full medium changes were performed daily ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
-
bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... specimens were post-fixed for 2 h at RT in 1% osmium tetroxide in 0.05 M cacodylate buffer pH 7.4 (Merck, Germany) and dehydrated stepwise in a graded ethanol series followed by 100% acetone ...
-
bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
-
bioRxiv - Genomics 2024Quote: ... ratio by a Gibson approach with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were preincubated for 1 h at RT in blocking buffer: 5% normal donkey serum (NDS, Vector) and 0.2 % Triton X-100 (Merck) diluted in PBS ...
-
bioRxiv - Biochemistry 2024Quote: ... for 4 h at 4°C and then concentrated to 200 ng/μl using Amicon Ultra-0.5 centrifugal filter units (Merck). Nucleosomes are then diluted with ice-cold 0 M FRET buffer to a concentration of 50 nM ...
-
bioRxiv - Genomics 2024Quote: ... and ligated by Gibson with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
-
bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
-
bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
-
bioRxiv - Bioengineering 2021Quote: Isolation of fresh human PBMCs was initiated within 1 h after blood collection using Histopaque® 1077 (10771; Merck KGaA) and standard density centrifugation (800 rcf ...
-
bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
-
bioRxiv - Bioengineering 2022Quote: ... Viral supernatant was harvested at 48 and 72 h post-transfection and filtered through a 0.45 μm PVDF membrane (Merck Millipore). For lentiviral infection ...
-
bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
-
bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
-
bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed and stained with indicated primary antibodies for 2 h at RT followed by incubation with PLA probes (Merck, Duolink® In Situ PLA® Probe Anti-Rabbit PLUS ...
-
bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were harvested after at least 24 h of incubation at 32°C and filtered on a 0.45 µm filter (Merck #SE2M230I04).