Labshake search
Citations for Merck :
1901 - 1950 of 2844 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Purification of His6-tagged proteins from the soluble fraction was achieved using Ni-NTA His.Bind® Resin (Merck), a peristaltic pump and a series of column washes with buffers of increasing imidazole content ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluted fractions containing purified protein samples were concentrated using a 10 kDa Amicon centrifugal filter (Merck Millipore) and the elution buffer suspending the purified protein was replaced with EDTA (10 mM)-PBS (1x ...
-
bioRxiv - Biochemistry 2020Quote: ... One and a half μl of protein solutions were mixed on the target plate with 0.5 μl of the 20% 2,5-dihydroxybenzoic acid (Merck) solution in 20% ACN ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein-RNA complexes were eluted in pull-down buffer supplemented with 20 mM maltose (Merck, cat. No. 63418). The eluates were loaded on a NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gel ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Purified protein was concentrated with the use of a 3K Amicon® Ultra 0.5 mL Centrifugal filter (Merck), according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Unbound proteins were removed by spin column purification (100kDa cut-off, Amicon® Ultra centrifugal filters (UFC210024, Merck)) ...
-
bioRxiv - Immunology 2023Quote: The I-Ek-specific 14.4.4 mAb was purified from hybridoma supernatant using a protein A/G column (Merck) and gel filtration (Superdex-200 10/300 GL ...
-
Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The total proteins were extracted by IP lysis buffer (Pierce) with a protease and phosphatase inhibitor cocktail (Merck), and then centrifuged at 12,000 g for 10 minutes at 4 °C to remove cell debris ...
-
bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... the purified protein was dialyzed against 20 mM HEPES using an Amicon ultra-15 (Merck, Rahway, NJ, USA).
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... and then protein was loaded into Superdex 200 and concentrated using Amicon Ultra-4 (3K cut off, Merck). The labeling percentage was calculated according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... and protein-containing fractions were pooled and concentrated using the 10 kDa molecular weight cut-off concentrator (Merck). For monomeric C9 ...
-
bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1X PPI] and precleared by 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h ...
-
bioRxiv - Biochemistry 2023Quote: ... Rgl2RA-Halo or GST- Rgl2RA/GST-BRAFRBD fusion proteins were concentrated using a concentrator (10 KDa MWCO, Merck, Amicon Ultra centrifugal filters ...
-
bioRxiv - Biochemistry 2023Quote: ... The fractions containing the target protein were concentrated to 5 mL with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 mL of HiLoad Superdex 75 (GE Healthcare Life Science ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated to 10 mL using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore) and loaded into SnakeSkin Dialysis Tubing ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell lysates (25 µL) and bovine serum albumin (BSA) protein standards (0-1 mg/mL BSA (Merck, Germany) in resuspension buffer ...
-
bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
-
bioRxiv - Plant Biology 2023Quote: ... Immunoblotting and detection were performed on the PVDF membrane with SNAP i.d.® 2.0 Protein Detection System (Merck) by using anti-myc (A00704 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... a small portion of the homogenate was used to determine the protein concentration using the Bradford dye (Merck) binding assay (Bradford ...
-
bioRxiv - Molecular Biology 2023Quote: ... This protein sample was concentrated 10 times using a 3.5 kDa Amicon Ultra 15 mL device (#UFC500324, Merck) at 3,000 x g 80 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... After the protein was collected the following day and concentrated using 10 kDa MWCO Amicon Ultra (Merck Millipore). The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated in 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
-
bioRxiv - Plant Biology 2023Quote: ... cells were transfected with plasmids encoding the respective heterologous proteins using the Genejuice transfection reagent (Merck, Darmstadt, Germany). After 48 hours of incubation at 37°C at 5% CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined and both fractions were incubated with Anti-FLAG® M2 Magnetic Beads (Merck, M8823). Beads were washed once then each sample was split for MS ...
-
bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
-
bioRxiv - Molecular Biology 2024Quote: ... and then incubated with pre-equilibrated protein G agarose plus anti-FLAG M2 (F3165, Merck, 1 μl/IP), or 5 μl/IP anti-GFP (A-11122 ...
-
bioRxiv - Biophysics 2024Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was incubated with uTEV3 protease25(Addgene ...
-
bioRxiv - Cancer Biology 2024Quote: Adhesion assay to different recombinant ECM proteins was performed using colorimetric ECM Cell Adhesion Array Kit (ECM540, Merck) plating 3×104 cells/well at 48h post-siRNA-transfection ...
-
bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was collected and concentrated using 100 kDa MW cut-off spin concentrators (Amicon Merck-Millipore) separated by size-exclusion chromatography (SEC) ...
-
bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore ...
-
bioRxiv - Pathology 2024Quote: ... proteins (120 μg for Commasie staining; 50 μg for western blotting) were diluted in Destreak Solution (Cytiva, Merck) plus 0.02% v/v pI 3–10 ampholytes (Bio-Rad) ...
-
bioRxiv - Biochemistry 2024Quote: ... Then the mixtures were concentrated to about 400 μM of protein concentration using Amicon Ultra-4 (Merck Millipore). Crystallization screening was performed using different commercially available crystallization screen by the sitting drop vapor-diffusion method at 4°C and 20°C ...
-
bioRxiv - Biophysics 2024Quote: ... The urified protein was concentrated using an Amicon® Ultra-4 centrifugal filter unit – 3000 NMWL (Merck-Millipore).
-
bioRxiv - Biophysics 2024Quote: ... Dialyzed protein was concentrated with Amicon Ultra 15 centrifugal filters (Regenerated cellulose 10,000 NMWL; Merck Millipore, Darmstadt, Germany). Protein concentration was determined by Pierce660 assay using bovine serum albumin as the standard ...
-
bioRxiv - Cell Biology 2024Quote: ... containing the GFP-FIP200(CTR) protein was concentrated using a 30 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
-
bioRxiv - Biochemistry 2024Quote: ... Fractions containing Nipah L-P protein were concentrated again using a MWCO 100,000 Amicon Ultra Centrifugal Filter (Merck). The protein with a final concentration of 1.5 uM was freshly used for grids preparation and/or aliquoted and flash-frozen and stored at 80 °C until use ...
-
bioRxiv - Cell Biology 2024Quote: ... Pelleted cells were stored in −80°C for protein measurement with QuantiPro™ BCA Assay Kit (Sigma/MERCK).
-
bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
-
bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
-
bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to buffer B without imidazole using 3 kDa Amicon® centrifugal filters (0.5 ml size) (both from Merck KGaA, Darmstadt, Germany).
-
bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
-
bioRxiv - Biophysics 2020Quote: ... at different concentrations (20 mM, 200 mM and 500 mM) and concentrated using centrifugal ultrafiltration devices (3 kDa cut-off) (Merck India Pvt. Ltd.). This was followed by Gel filtration chromatography using Superdex 200 Increase 10/300 GL column (GE Lifesciences ...
-
bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck, Buchs Switzerland). Memofl/fl littermates without Cre but treated with tamoxifen served as controls ...