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Citations for Merck :
2151 - 2200 of 2844 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the appropriate HRP-conjugated secondary antibody (goat-anti-rabbit, AP307T, Merck (Darmstadt, Germany), 1:13000 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... immediately euthanised in a petri-dish filled with overdosed tricaine methanesulfonate (MS-222, ethyl 3-aminobenzoate methanesulfonate salt; Merck/Supelco CAT#A5040, 200 mg/L), and quickly imaged (see dedicated section) ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Genetics 2021Quote: ... For western blotting the proteins were resolved in SDS-polyacrylamide gels and transferred electrophoretically at room temperature to PVDF membranes (Merck) for 1 h at 50 mA using a Tris-glycine buffer system ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted NlGr7 protein fractions were applied on a 10 kDa cut-off Centricon centrifugal device (Merck Pte. Ltd., Germany) for desalting against buffer (25 mM Tris–HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleaved NlGr7 protein was concentrated by using a 10 kDa cut-off Centricon centrifugal device (Merck Pte. Ltd., Germany) and was purified by affinity chromatography on amylose resin (NEB ...
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bioRxiv - Biophysics 2022Quote: ... and monomeric protein was purified by size-exclusion chromatography using a HiLoad™ 26/600 Superdex™ 75 pg (Merck) equilibrated with 25 mM HEPES ...
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bioRxiv - Physiology 2020Quote: ... Fusion proteins were exchanged into low (< 30 mM) imidazole buffer using an Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany) with a 30 kDa molecular weight cut-off in preparation for liberation of Hi1a from the fusion tag using tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchange to PBS and the concentration of the protein were done by using amicons® (Merck Millipore Ltd, cat:UFC900324).
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bioRxiv - Biochemistry 2020Quote: ... The recombinant protein was dialyzed overnight using an Amicon Ultra 15 mL spin dialysis column 10 000 MWCO (Merck, Germany). Purified protein was quantified using Bradford’s assay ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...
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bioRxiv - Biochemistry 2021Quote: ... the pure huPrP protein (i.e., with the HisTag removed) was concentrated using an Amicon Ultra 0.5 ml centrifugal filter (Merck & Co., USA) with an NMWL cutoff of 3 kDa ...
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bioRxiv - Biophysics 2021Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated using amicon 10 k MWCO centrifugal filtration devices (Merck). Protein purity was determined by aRP to be 88.6% (Supplementary Figure 8) ...
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bioRxiv - Molecular Biology 2020Quote: ... 30~50 μg of protein samples were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck) using an Amersham semi-dry transfer system ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using Millex Non-Sterile Low Protein Binding Hydrophilic LCR (PTFE) Membrane (0.45 µm) filters (Merck #SLLHR04NL) into 1.5 mL MS vials with silicone/PTFE septa and stored at -20 °C for later analysis.
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 40 mM Chloroacetamide (CAA) at RT for 30 min and protein amount was quantified using the Direct Detect Spectrometer from Merck. Protein digestion was performed using the Single-Pot Solid-Phase-enhanced Sample Preparation approach SP3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin and its associated proteins were solubilized by incubation with 4- 20 units of Micrococcal nuclease (MNase; Merck Life Sciences) for 10 minutes at 37° C ...
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bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was collected from the supernatant of cultured cells and concentrated by Amicon Ultra-15 Centrifugal Filter Unit (Merck). Then the fusion protein was purified using BeaverBeads IDA-Nickel Kit (Beaverbio) ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µg of protein solution was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 ...
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bioRxiv - Immunology 2020Quote: ... The proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto an Immobilon-P membrane (IPVH00010; Merck). The membranes were blocked with 10% nonfat milk (190-12865 ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were incubated with anti-HA agarose beads (20 μl of beads per 1 mg of proteins) (Merck, Darmstadt, Germany) for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were concentrated by centrifugation using spin columns (MW cut off of 10 kDa) (cat number UFC501096– Merck Millipore). Protein concentration was estimated by UV using calculated extinction coefficients (42,400 mol/l-1.cm-1) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Plant Biology 2023Quote: ... Transfection with plasmids carrying the coding sequences of the indicated proteins was performed using Genejuice transfection reagent (Merck, Darmstadt, Germany). 48 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Membranes were blocked for one hour in I-BlockTM (# T2015 ...
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bioRxiv - Plant Biology 2024Quote: ... Apoenzyme preparations of D-AAT and AtPLPHP1 were achieved by incubating 100 μM of purified protein with 75 mM D-cycloserine (Merck) in 50 mM HEPES pH 7.4 containing 300 mM sodium chloride on ice for 2 hours followed by buffer exchange as described above ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity-purified recombinant TrK13 proteins (WT, C580Y, R539T and C580Y) or commercially purchased BSA (A7030) or lysozyme (L6876) (Merck, Germany), each at 10 µM concentration ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Total IgG were isolated from post-immune sera by affinity purification on Sepharose-protein G (Merck, Cytiva 17-0618-05). Custom sheep pAb anti-pS55 and anti-pS44 NDC80 pAb was raised by the Scottish Blood Transfusion Service using a 4-month long program ...