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Citations for Merck :
1701 - 1750 of 3953 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM MgCl2) containing 1% BSA and incubated overnight with the G4-specific antibody (BG4, MABE917, Merck) at 500 ng/mL in BG4 buffer containing 1% BSA ...
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bioRxiv - Genetics 2019Quote: ... rabbit anti-TbRBP16 [1:1,000] (27) and mouse anti-EF1a [1:200,000] (clone CBP-KK1, Merck-Millipore) for 18 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... in the presence of enzymatic digestion solution containing 1 mg ml-1 collagenase II (Merck Millipore, 234155), 1 mg ml-1collagenase IV (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked for 1 hour in PBS supplemented with 1% Tween-20 (Merck MP Biomedicals) and 5% skimmed milk (Friesland Campina) ...
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bioRxiv - Developmental Biology 2022Quote: ... different concentrations from 1:10 to 1:10000 (V/V) were freshly diluted in absolute ethanol (Merck). For NaCl chemotaxis ...
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bioRxiv - Cell Biology 2022Quote: ... 1:600-diluted rabbit anti-CENP-C (#554),55 mouse anti-HP1α (1:1,000, #MAB3584, Merck Millipore), rabbit anti-HP1β (1:800 ...
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bioRxiv - Microbiology 2023Quote: ... diluted 1 : 100 in 1 × PBS buffer (pH 7.5) with 0.1 % hydrogen peroxide (stock concentration 30 %, Merck) and 2 mM ATP (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Trypsinise Ara-C purified SCs using 1 ml of 6% 2 mg ml-1 Trypsin (Merck - 85450C) in Versene (0.02% EDTA (Thermo Fisher - D/0700/53 ...
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bioRxiv - Plant Biology 2024Quote: ... the blots were incubated with primary antibodies α-FLAG (Merck, F1804, 1:1000], α-TPLATE34(1:1000), α-AtEH1/Pan135(1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Ki67 (rabbit, 1:400, CST, Cat. no 9661S) and cCas3 (rabbit, 1:600, Merck, Cat. no AB9260). The cells were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2024Quote: ... Postfixation was applied using 1% Osmiumtetroxid (Science Services, München, Germany) and 1% Potassium hexacyanoferrat (Merck, Darmstadt, Germany) for 30 min at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... Constructs of Kmpv1 and KmpvSP1 were transiently expressed as fusion proteins with GFP on the C-terminus using the liposomal transfection reagent GeneJuice® (MERCK KGaA, Darmstadt, Germany). Measurements were performed at room temperature in a bath solution containing ...
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bioRxiv - Bioengineering 2019Quote: ... 40 µg of total protein were run on a 12% SDS-PAGE and transferred onto a 0.45 µm Immobilon®-P Transfer Membrane (Merck Millipore, Cat. NO#IPVH00010). The membrane was hybridized with a custom antibody at a 1 µg/mL dilution (GenScript ...
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS- PAGE and those containing pure tag free CBX8 protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer consisting of 20mM MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL).
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions were analyzed for purity by SDS-PAGE and those containing pure protein were pooled and concentrated using Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Protein was exchanged into a buffer containing 25 mM Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated to the desired concentration using centrifugal filters (#UFC800308; Amicon® Ultra centrifugal filters, Ultracel® −3K or larger; Merck Millipore Ltd.)
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bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The protein complexes were concentrated to 0.7-1 mg ml-1 using an Amicon Ultra-0.5 mL concentrating device (Merck) with a 100 kDa filter cut-off ...
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bioRxiv - Genetics 2019Quote: ... These were then re-solubilized in 30μl chloroform/methanol (1:1) and loaded onto silica gel plates (Merck). PA and DAG were separated by 10-15min of thin layer chromatography (TLC ...
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bioRxiv - Microbiology 2019Quote: ... Antibody staining was performed using EV-A71 monoclonal antibody MAB979 (Merck, USA; 1:2500 dilution in 1 % BSA) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM EDTA and 1 mM DTT and concentrated with Amicon Ultra-0.5 mL centrifugal filter (Merck #UFC503096). Nucleosomes were stored in 4 °C and the quality of nucleosomes was assessed by 5-6 % TBE gel.
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:1 dilution with tris-buffered saline (TBS) containing 0.1% (v/v) Tween® 20 (Merck). Subsequently ...
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bioRxiv - Biophysics 2022Quote: ... All pH adjustments were performed using 1 M HCl or 1 M NaOH (99.0 %, Merck KGaA, Darmstadt, Germany). All samples were filtered prior to stress application through a 0.1 µm inorganic membrane filter (#6809-2012 ...
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bioRxiv - Immunology 2020Quote: ... rAAVDJ or rAAV6 genome plasmid and Donor plasmid at a 3:1:1 ratio in Polyethylenimine (PEI)(Merck). In total each plate was teransfected with 41,250ng of DNA ...
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bioRxiv - Microbiology 2022Quote: ... the samples were mixed vigorously at a ratio of 1:1 with saturated phenol solution pH 4.3 (Merck) and centrifuged for 15 min at 13’000 rpm ...
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bioRxiv - Plant Biology 2019Quote: ... 0.8 % phytoagar (Duchefa) and 10 µg mL-1 Glufosinate-ammonium or 25 µg mL-1 hygromycin B (Merck) for herbicide selection ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mM imidazole and 1 mM TCEP) supplemented with protease inhibitor cocktail EDTA-free (Merck Millipore; 1:200). Cells were stored at −80°C until use ...
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bioRxiv - Neuroscience 2022Quote: ... FITC-conjugated (1:200; F7367 or F7512) or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with protease inhibitor cocktail set III (Cat No. 539134-1 set, 1:100, Merck Millipore, Rahway, NJ). The lysate was centrifuged at 16,000 g at 4 LJ for 10 minutes to remove insoluble samples ...
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bioRxiv - Cell Biology 2023Quote: ... or propranolol hydrochloride (20 mg·kg-1 for the first five days and 10 mg·kg-1 from day six until euthanasia, Merck), diluted in 0.9% saline ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were lysed in lysis buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 0.5% Triton X, 1 x cOmplete protease inhibitors [1x = 1 tablet in 40mL; Merck]), sonicated ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1h30min at RT with primary antibodies Centrin-1(20H5) (1/1000, Merck 04-1424) and CP110 (1/250 ...
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bioRxiv - Molecular Biology 2023Quote: ... exponentially growing RPE-1 cells were pulse-labeled with 1 μM CldU (5-Chloro-2’-deoxyuridine, Merck, C6891) and 250 μM IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × 107 wild-type U2OS or AsiSI-ER U2OS cells were crosslinked with 1% formaldehyde (Merck, 252549-100ML) for 10 min at 37°C and inactivated by the addition of glycine (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-MAP1B (Merck #MAB366, 1:200); rabbit anti-cleaved-CASP3 (Cell Signaling #9664 ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-MAP1B (Merck #MAB366, 1:500); mouse anti-TRIM9 (Abnova #ABN-H00114088-M01 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% aqueous eosin (VWR, Merck, Birmingham, UK) with 1% calcium carbonate (Sigma ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and ACTB (1:25,000, #A3854, Merck KGaA) were used ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium pyruvate (Merck KGA, Germany), and 1% penicillin/streptomycin (Merck KGA ...
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bioRxiv - Cell Biology 2020Quote: ... Anti LBPA 6C4 (Merck Millipore; 1:100), Anti LC3B (D11 ...