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Citations for Merck :
1501 - 1550 of 3953 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1:10000 (Sigma-MERCK, G9545); anti-ATG5 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 (Sigma-MERCK, HPA007244); anti-acetylated tubulin ...
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bioRxiv - Neuroscience 2020Quote: ... rat polyclonal anti-Vangl2 (clone 2G4, 1:200 for Western blotting, 1:400 for immunoprecipitation, 1:500 for immunostaining, Merck Millipore, catalog no. MABN750), mouse monoclonal IgG1k anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Bioengineering 2021Quote: ... and stained with 1 mL of 1 mg/mL of Direct red solution (Merck KGaA) in saturated aqueous solution of picric acid (Merck KGaA ...
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bioRxiv - Neuroscience 2020Quote: ... guinea pig anti-vGluT1 and -vGluT2 (1:2000; Chemicon) and anti-vGluT3 (1:2000; Merck), mouse anti-Lhx6 (1:X ...
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bioRxiv - Microbiology 2019Quote: ... polyclonal α-Hpf antibody (1:5000) [69] or monoclonal anti-puromycin antibody (1:10.000, Merck) and HRP-conjugated anti-mouse or anti-rabbit antibodies (1:10.000 ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal pS129 (11A5, 1:10,000) and tyrosine hydroxylase (TH, #AB152, Merck Millipore, 1:1000). Appropriate secondary Alexa Flour antibodies (Life Technologies and Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then diluted 1:1 (v:v) with water containing 4mM MgCl2 and Benzonase (Merck #70746 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or 1-octanol was used (1-OCT; CAS: 111-87-5; Merck, Darmstadt, Germany; undiluted). Paraffin is without behavioural significance in larval Drosophila (Saumweber et al ...
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bioRxiv - Cell Biology 2023Quote: ... with protease inhibitor cocktail (1:1,000) and phosphatase inhibitor cocktail (1:400; Sigma-Aldrich/Merck) by sonication (VCX 130 PB ...
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bioRxiv - Microbiology 2024Quote: ... anti-CycB (sc-245, Santa-Cruz, 1:300) or anti-tubulin (T5168, Merck, 1:40000) and the following secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then process for mass spectrometry or the proteins were transferred to the polyvinylidene difluoride membrane (Merck Millipore, Cat. No. IPVH00010) by Western Blotting (WB) ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Biophysics 2021Quote: ... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatant containing the sheared chromatin was pre-cleared with Protein G plus-Agarose beads (Cat No. IP04-1.5ML, Merck Millipore, Burlington, USA), which were pre-coated with bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: For phosphorylated proteins RIPA lysis buffer was further supplemented with PIC and 1X phosphatase inhibitor cocktail (Merck Millipore, cat no. 524629-1ML). A cocktail of phosphatase inhibitors (B-Glycerolphosphat (25mM) ...
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bioRxiv - Biochemistry 2021Quote: RNA immunoprecipitation (RIP) assays were conducted using the EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck Chemicals (Shanghai Co., Ltd). The anti-YTHDC1 antibody for RIP was purchased from Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were fractionated on 8% (w/v) sodium dodecyl sulfate polyacrylamide gels and transferred to Immobilon®-P PVDF Membrane (Merck, IPVH00010). Membranes were incubated in 5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The tissue was processed as previously reported [26] and proteins were loaded onto 10% polyacrylamide gels and transferred to PVDF sheets (Immobilion-P, MERCK, Burlington, USA). Membranes were blocked with BSA 5% and immunoblotted using primary antibodies for β-Tubulin ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing protein were combined and a buffer exchange and the protein were concentrated with a 30 K Amicon® Ultra Centrifugal Filter spin column (Merck KGaA) to the storage buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were then transferred by wet tank-blotting (100 V for 60 min) onto a polyvinylidene difluoride (PVDF) membrane (Merck, Darmstadt, Germany). The membrane was blocked with Roti®-Block (2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... at pH 2.0 with final protein concentration of 1.75 mg/mL (Sphβ preparation) or in 40% ethanol (v/v from ethanol absolute, Merck KGaA, Darmstadt, Germany), 0.25 M NaCl at pH 1.8 with final protein concentration of 5 mg/mL (Sphα preparation) ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein from tissue extracts or plasma were prepared in Laemmli or RIPA buffers (both from Merck Life Science UK Ltd, UK), separated by polyacrylamide gel electrophoresis and then transferred to either nitrocellulose or a polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... diluting 3 μl of protein sample (20 nM) in 15 μl PBS buffer loaded on a gasket (Grace Bio-Labs reusable CultureWell gaskets (GBL103250, Merck)) mounted on a glass microscope coverslip (0107222 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Immunology 2023Quote: ... Equal amounts of protein were loaded and separated by electrophoresis on 12% SDS-PAGE gels and subsequently transferred to polyvinylidene fluoride (PVDF; Merck-Millipore, Germany). After blocking with 10% skim milk for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2019Quote: ... For BN-PAGE samples were lysed in 1 % digitonin buffer (1 % digitonin (Sigma/Merck, Darmstadt, Germany) in 20 mM Tris–HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 U ml−1 penicillin and 50 μg ml−1 streptomycin (Merck Life Science (Sigma)) at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... ampicillin (50 µg mL-1) (Polfa Tarchonin) and cyclocheximide (100 µg mL-1) (Sigma-Aldrich/Merck) were supplemented to the media as selective agents.
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bioRxiv - Microbiology 2023Quote: ... 1% (w/v) sodium dodecyl sulfate and 1 mg/mL proteinase K (Merck Millipore, Darmstadt, Germany) were added to the fecal pellets and incubated at 55°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of hGCase-1/17 or hGCase-1/23 or mouse IgG (Merck Millipore, #NI03) were coupled to 50 µl of Protein A magnetic Dynabeads in 200 µl Ab Binding and Washing Buffer for 30 minutes at RT on a rotating wheel ...
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bioRxiv - Cancer Biology 2024Quote: ... counterstained with 5µg of 4’,6-diamidino-2-phenylindole (DAPI – 1:1) (Sigma/Merck, D9542-10mg) to eliminate dead cells before running through the flow cytometer ...
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bioRxiv - Immunology 2021Quote: ... EmbryoMax Nucleosides (1% v/v, Merck), and 100 U/ml penicillin and 100 μg/ml streptomycin (SigmaAldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... and DAPI (1 µg/mL, Merck) in blocking buffer for 3 h at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... and DAPI (1 µg/mL, Merck) in blocking buffer for 4 h at 4 °C ...