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Citations for Merck :
1701 - 1750 of 2734 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a Immobilion®-P Transfer Membrane (0.45 µm pore size, Cat#: IPVH00010, Merck) and blocked for 1h at RT in 1xTris Buffered Saline Tween (TBST ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Microbiology 2024Quote: ... Target protein-containing fractions were combined and concentrated using an Amicon Ultra-4 50 kDa filter (Merck) operated at 7,500 x g and 4 °C ...
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bioRxiv - Physiology 2023Quote: ... 20 μg of total protein were transferred onto centrifugal filter units (Microcon-30 kDa; Merck, Darmstadt, Germany) and washed with a buffer containing 8 M urea and 100 mM Tris (pH 8.5) ...
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bioRxiv - Plant Biology 2024Quote: ... and proteins were concentrated using a 30 kDa Amicon Ultra-4 Centrifugal Filter Unit (Merck Milipore; www.merckmillipore.com). Protein concentration was determined employing the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The fractions with target proteins were pooled and concentrated using an Amicon Ultra 3K NMWL (Merck Millipore). The sample was ultracentrifuged (45,000 rpm x 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing 20 nmol phosphate were subjected to TLC on an HPTLC plate (Silica gel 60; Merck Millipore, MA, USA) in the solvent system hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... We dissolved dried lipid extracts in 40 μL of 1:1 methanol:CHCl3 and used 5-10 μL of sample for thin layer chromatography (TLC) on TLC silica gel 60 plates (Merck, Germany) with a mixture of 65:25:10 CHCl3:CH3OH:CH3COOH for the mobile phase ...
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bioRxiv - Biochemistry 2019Quote: ... An aliquot of 10 μL of the reaction mixtures were spotted on HPTLC (High Performance TLC) Silica Gel 60 Glass Plates (Merck KGaA), and the plates were developed with a phenol:n-butanol:formate:water (12:3:2:3 v/v ...
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bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Neuroscience 2020Quote: ... the beads were suspended in 100 μl Sheath fluid and transferred to the 96 well flat bottom plate to be read on the FlexMap 3D system equipped with xPONENT software (Merck, Germany). The median fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2021Quote: ... Cell pellet was resuspended in medium and cells were seeded with fresh medium on plates or coverslips (Corning, Merck, Darmstadt, Germany) coated with PLO (0.1 mg/mL in borate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Biochemistry 2021Quote: ... Dried lipids were resuspended in 150 ml of CHCl3 and spotted on silica gel high-performance–TLC (HPTLC) plates (Merck, Germany) and resolved with a mixture of chloroform ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were grown in 24-well plates and treated with increasing concentrations of CaCl2 and NaH2PO4 · Na2HPO4 alone or in combination with PPi (Merck, #P8010) and/or phosphatase (Prospec ...
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bioRxiv - Plant Biology 2021Quote: ... lipid extracts were roughly separated into polar and non-polar lipids by thin-layer chromatography (TLC) using silica plates (TLC silica gel 60, Merck Millipore) developed with chloroform because the high amount of shikonin derivatives hampered the chemical analysis of TAG and polar lipids by LC-MS ...
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bioRxiv - Plant Biology 2021Quote: ... 80 µL of the dissolved samples were spotted with a TLC spotter to TLC plates (TLC Silica gel 60, Merck KGaA). For extraction of TGs ...
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bioRxiv - Microbiology 2020Quote: ... A serial dilution of the homogenized material was plated out onto blood agar (Substrat, Sahlgrenska Universitetssjukhuset, Sweden) and Thiosulfate-citrate-bile salts-sucrose agar plates (86348, Merck, Germany). The number of Vibrio cholerae CFU per intestine was calculated.
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bioRxiv - Microbiology 2021Quote: ... structured microcosms were set-up in multi-well plates with a 0.22 µm filter membrane at the bottom of the well (Merck, Darmstadt, Germany) and containing 250 mg of sterile glass beads (with diameter of 80 to 120 µm ...
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bioRxiv - Microbiology 2020Quote: The crude extract and purified fractions were separated on TLC Silica gel 60 F254 plates (Merck Life Science Pvt. Ltd., Germany) using the solvent system of hexane ...
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bioRxiv - Microbiology 2019Quote: ... Dried samples were resuspended in 100 µL of hexane and aliquots were transferred on the TLC Silica gel 60 plates (Merck, Germany). Plates were resolved for approximately 30 minutes in hexane ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 10 μL were spotted into reverse-phase TLC (RP-TLC) plates (silica gel 60, RP-18, F254s, 1 mm, Merck, Germany). The plates were developed in chambers pre-saturated for 10 min using acetone as a solvent system ...
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bioRxiv - Biochemistry 2021Quote: Separation of phospholipids by 2D-TLC Total lipid extracts corresponding to 200 nmol phospholipid were applied on silicagel plates (Merck 1.05641), freshly impregnated with 2.4% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... salicis was stored in glycerol stock at −80 °C and reactivated on tryptic soy broth (TSB) plates (Art. No. 100550, Merck KGaA). After 24 h incubation at 22 °C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of 10 µl of the reaction mixtures is spotted on HPTLC (High-Performance TLC) Silica Gel 60 Glass Plates (Merck KGaA) after centrifugation at 16.000 g for 2 min ...
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bioRxiv - Immunology 2021Quote: ... 20 μl of this cell/collagen mixture was added in one well of pre-cooled Corning™ 96-Well half-area plate (Merck) and the plate was centrifuged ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed by incubation with 2% Saponin in HBSS and lysates were serially diluted in 0.05% Tween-80 / dH20 and plated on 7H10 agar plates containing 10% heat-inactivated bovine serum (Merck, Darmstadt, Germany). Plates were incubated for 3 to 4 weeks at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Injections were performed as a single injection targeting the motor end plate region (Mohan et al., 2014) in a volume of ∼3.5 μl using a 701N Hamilton® syringe (Merck, 20779) for TA and LG ...
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bioRxiv - Synthetic Biology 2022Quote: Cells were grown in CellASIC ONIX microfluidic plates for haploid yeast cells in media controlled by the ONIX2 microfluidics system (Merck, Germany). Details regarding the composition of the media used for different promoter induction experiments are given in Supplementary Note 1.
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Cell Biology 2022Quote: ... The full-thickness (epidermis and dermis) RHS was developed in-house in a 24-well plate size insert (Millicell-PCF, Merck, Brazil), and it was fully differentiated after ten days ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the experiments were performed in 96-wells plates containing 100 μL of MM (with 1% agar) supplemented or not with 3mM of H2O2 (Merck S.A) or 0.15mM of menadione (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: Thin-layer chromatography (TLC) was performed using silica gel 60 GF254 precoated aluminium backed plates (0.25 mm thickness; Merck, Darmstadt, Germany), and visualization was accomplished by irradiation with UV light at 254 nm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... All reactions were monitored by LC/MS analysis or by thin-layer chromatography (TLC) using aluminium plates precoated with silica gel (silica gel 60 F254, Merck, US) impregnated with a fluorescent indicator ...
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bioRxiv - Plant Biology 2023Quote: ... Diluted samples were filtered through a 0.22 μm PVDF filter (MultiScreenHTS GV Filter Plate, 0.22 μm, clear, non-sterile, Merck Millipore, Billerica, USA) and stored at −70°C until further analysis.
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bioRxiv - Microbiology 2023Quote: ... cryopreserved PBMCs from participants treated in vivo with ALN or PLACEBO were thawed and rested overnight then added to ELISpot plates (Merck, Millipore) in quadruplicate (1 or 1.25 × 105 PBMC/ well ...
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bioRxiv - Neuroscience 2023Quote: ... cells were split into either 6 well-plates (300000 cells per well) coated with 1 mg/ml Poly-L- Lysine (PLL, Merck, P9155) for western blotting ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were freshly grown for 20 to 24 h on plates in anaerobic jars supplemented with Anaerocult C sachets (Merck, USA) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 to 200,000 HEK293-EBNA cells were plated in 1 mL complete DMEM per well of 12-well cell culture plates (#665180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...