Labshake search
Citations for Merck :
1951 - 2000 of 2965 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: The I-Ek-specific 14.4.4 mAb was purified from hybridoma supernatant using a protein A/G column (Merck) and gel filtration (Superdex-200 10/300 GL ...
-
Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The total proteins were extracted by IP lysis buffer (Pierce) with a protease and phosphatase inhibitor cocktail (Merck), and then centrifuged at 12,000 g for 10 minutes at 4 °C to remove cell debris ...
-
bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... the purified protein was dialyzed against 20 mM HEPES using an Amicon ultra-15 (Merck, Rahway, NJ, USA).
-
bioRxiv - Neuroscience 2023Quote: ... and then protein was loaded into Superdex 200 and concentrated using Amicon Ultra-4 (3K cut off, Merck). The labeling percentage was calculated according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined and both fractions were incubated with Anti-FLAG® M2 Magnetic Beads (Merck, M8823). Beads were washed once then each sample was split for MS ...
-
bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... a small portion of the homogenate was used to determine the protein concentration using the Bradford dye (Merck) binding assay (Bradford ...
-
bioRxiv - Molecular Biology 2023Quote: ... This protein sample was concentrated 10 times using a 3.5 kDa Amicon Ultra 15 mL device (#UFC500324, Merck) at 3,000 x g 80 minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... After the protein was collected the following day and concentrated using 10 kDa MWCO Amicon Ultra (Merck Millipore). The protein concentration was monitored periodically during the process using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
-
bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
-
bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore ...
-
bioRxiv - Biophysics 2024Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was incubated with uTEV3 protease25(Addgene ...
-
bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was collected and concentrated using 100 kDa MW cut-off spin concentrators (Amicon Merck-Millipore) separated by size-exclusion chromatography (SEC) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and then incubated with pre-equilibrated protein G agarose plus anti-FLAG M2 (F3165, Merck, 1 μl/IP), or 5 μl/IP anti-GFP (A-11122 ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were normalized according to the number of worms and applied to 10 cm HPTLC silica gel 60 matrix plates (Merck, Darmstadt, Germany). For lipid analysis ...
-
bioRxiv - Biochemistry 2020Quote: ... Two μM of the reaction mixture was spotted onto a thin layer chromatography (TLC) Silica Gel 60 F254 plate (Merck, Darmstadt, Germany). Subsequently ...
-
bioRxiv - Microbiology 2021Quote: Estrogen metabolites were separated on silica gel aluminum thin-layer chromatography (TLC) plates (Silica gel 60 F254: thickness, 0.2 mm; 20 × 20 cm; Merck, Darmstadt, Hesse, Germany). Dichloromethane:ethyl acetate:ethanol (7:2:0.025 ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were plated at a density of 9000 cells/cm2 in PDL and laminin-coated 96 well Nunclon® plates (Merck, P8366), stained for βIII-Tubulin (Tuj1 ...
-
bioRxiv - Biochemistry 2021Quote: ... free radioligands bound to DCC were removed using a vacuum filtration system with a 96-well filtration plate (MultiScreenHTS HV, 0.45-mm pore size, Merck KGaA, Darmstadt, Germany) for the bound/free (B/F ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The residues were reconstituted in ethyl acetate and quantitatively applied to silica gel G thin-layer plates (Kieselgel G 60/DC-Fertigplatten, Merck, Art. 5721). The plates were developed to a distance of 16 cm in the organic phase of ethyl acetate:acetic acid:2,2,4-trimethylpentane:water (110:20:30:100 ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were monitored by thin layer chromatography (TLC) using TLC plates pre-coated with silica gel 60 F254 (Merck KGaA, Darmstadt, Germany), visualized with a handheld ultraviolet device either directly or after staining with 5% H2SO4 in ethanol ...
-
bioRxiv - Microbiology 2019Quote: ... Putative Salmonella colonies were selected by black appearance on XLD plates and confirmed by pink and white colony formation on Brilliant Green Agar (Merck, 70134-500G) supplemented with 0.35 g/L mandelic acid (Merck ...
-
bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
-
bioRxiv - Microbiology 2020Quote: ... each coverslip was carefully removed from the plates and attached (side opposite to biofilm) on the surface of a regular glass slide using Entellan mounting medium (Merck, Darmstadt, Germany) for subsequent scanning as below.
-
bioRxiv - Microbiology 2020Quote: Filter to remove host bacteria by transferring 200 µl (punch through pierceable tape) from each well to a 96-well filter Plate (0.45 µm) (e.g. MSHAS4510, Merck Millipore, Burlington MA US), pipette up and down a few times before extracting ...
-
bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
-
bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
-
bioRxiv - Cell Biology 2024Quote: ... 50 µL (= 5 mg fresh weight) of total lipids were applied onto a silica-coated chromatography plate (Silicagel 60 F254, 10x 20 cm; Merck, Rahway, NJ) and developed with hexane/ethyl ether/acetic acid (21 ...
-
bioRxiv - Immunology 2024Quote: Thin-layer chromatographic (TLC) analysis of lipids was performed on chromatographic plates (TLC Silica gel 60 F254; Merck KGaA 64071, Darmstadt, Germany) according to our previously published protocol20.
-
bioRxiv - Microbiology 2024Quote: ... standard dilution or sample was added into the wells of a 96-well clear bottom plate and 250 μL of Bradford reagent (Merck, Darmstadt, Germany) was added ...
-
bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293A cells transfected with G-CASE and pcDNA or N-terminally FLAG-tagged H1/2R constructs were grown for 48 hours in transparent 96-well plates (Brand, Wertheim, Germany) and washed once with 0.5% BSA (Merck KGaA, Darmstadt, Germany) in PBS ...
-
bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
-
bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
-
bioRxiv - Plant Biology 2023Quote: ... An aliquot of each sample was subjected to TLC using a silica gel 60 F254 aluminum TLC plate (Merck Millipore, Burlington, USA), using a running buffer containing ethyl acetate/acetic acid/methanol/formic acid/water at a ratio of 80:40:10:10:10 (v/v) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All moisture-sensitive reactions were carried out in oven-dried glassware under N2 Thin-layer chromatograms (TLC) and flash chromatography separations were respectively performed on precoated silica gel 60 F254 plates (Merck, 0.25 mm) and on silica gel 60 (230-400 mesh) ...
-
bioRxiv - Microbiology 2023Quote: Quantification of polar lipids content on HPTLC was carried out as follows: Lipid extracts and polar lipids standards at varying concentrations were applied onto silica gel 60 F254 HPTLC plates (Merck KGaA, Germany) using an ATS 5 automatic TLC sampler (Camag ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 2 mM DTT were heated in a LightCycler® 480 Multiwell white 384-well plate in a Roche LightCycler® 480 II from 20 to 80°C in the presence of SYPRO™ Orange (Merck) diluted 1:160.
-
bioRxiv - Cell Biology 2020Quote: ... pDEST42-Ctdnep1_C-ter plasmid was transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore), Bacteria cultures (500 ml ...
-
bioRxiv - Cell Biology 2020Quote: A549 cells were treated with 2 ng/ml TGF-β1 (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 2 days in DMEM with 10% FBS on non-treated culture dish surfaces (31 ...
-
bioRxiv - Microbiology 2019Quote: ... cleared by xylene (2 x 5 min) and finally mounted in Entellan mounting medium (Merck, Germany). HE stained sections were observed and recorded using an ECLIPSE Ni microscope (Nikon ...
-
bioRxiv - Immunology 2020Quote: ... After each cycle the flow channels were regenerated for 10 seconds with 2 M NaCl (Merck) at 10 µL/min ...
-
bioRxiv - Bioengineering 2021Quote: ... with Pellicon 2 mini cassettes (300 kDa, 0.1 m2, BioMax polyethersulfone membrane with C-screen; Merck), as previously described (4) ...
-
bioRxiv - Biophysics 2021Quote: ... Cells at 2-3 x 106 cells/mL were induced with 50 ng/mL doxycycline (Merck) and 5 mM valproic acid (Cayman Chemical) ...
-
bioRxiv - Cancer Biology 2020Quote: ... media was replaced with media containing 10 nM 5-Bromo-2′-deoxyuridine (BrdU) (Merck, B5002-100MG). Coverslips were then fixed ...