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Citations for Merck :
1701 - 1750 of 2176 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and 10% ultra-pure water from Direct-Q 3 UV Water Purification System with LC-Pak Polisher (Merck KGaA) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated for 24 hours in blocking buffer containing 10% (vol/vol) normal donkey serum (NDS) (S30-100ML, Merck), 0.5% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 10 minutes in Toluene and coverslipped in a mixture of Toluene and Entellan (Merck KGaA). Another mounted series was used for Nissl staining with Cresyl Violet (Sigma® Life Science ...
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bioRxiv - Cell Biology 2022Quote: Hela Kyoto and FERRY subunit knock-out cells were cultured in DMEM media supplemented with 10% FBS Superior (Merck) and 50 μg/ml streptomycin (P/S ...
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bioRxiv - Molecular Biology 2024Quote: ... then layered over 5ml of 70% Percoll in a 15ml tube (Percoll Merck P4937, 10% 10x PBS, 20% RPMI). The tube was then centrifuged at 1450rcf for 11mins with the break set to 1 and accelerator to 3 ...
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bioRxiv - Cell Biology 2023Quote: ... protein thioesterase inhibitors (10 µM palmostatin and 0.2 mM HDSF (1-hexadecanesulfonyl fluoride)) and 250 U/ml Benzonase Nuclease (Merck). After 30 min (4°C) ...
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bioRxiv - Biochemistry 2024Quote: ... Purified MPP8 CTD was concentrated to 4.5 mg/ml using a 10-kDa cutoff centrifugal filtration device (Merck Millipore) and mixed with an equal volume (up to 0.5 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Protease inhibitor was added to sample and sample was concentrated down to 150 µL using Amicon Ultra-0.5 10 kDa Centrifugal Filter Units (Merck). On the other hand ...
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bioRxiv - Biochemistry 2024Quote: The lysate was cleared by centrifugation at 12,000g for 30min at 10°C and the supernatant was applied onto a Talon Superflow beads batch (Merck). The immobilized proteins were washed with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: Unless otherwise noted the standard assay was carried out at 65 °C for 10 min in 500 μL reaction mixture using 0.25 % (w/v) laminarin from Laminaria digitata (Merck) as substrate in 50 mM sodium phosphate buffer pH 6.0 (adjusted at assay temperature) ...
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bioRxiv - Genomics 2024Quote: ... Cells were then resuspended in 100µL RPMI + 10% FCS and stimulated for 4h with PMA (100ng/mL, Merck, P8139) and ionomycin (1µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... the TurboID-CpOGACD or TurboID-CpOGADM expressing HEK293T cells were labeled with 10 to 100 µM biotin (Merck, B4501) in the medium for 15 min to 3 h ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was filtered through Amicon® Ultra – 0.5 mL 10-kDa cutoff centrifugal filters (Merck KgaA; Darmstadt, Germany). To test the enzymatic efficiency of DisA before synthesizing [32P]c-di-AMP ...
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bioRxiv - Microbiology 2023Quote: ... The purified fractions were desalted and concentrated using an Amicon Ultra centrifugal filter unit (10-kDa cutoff; Merck Millipore), and the enzyme preparations were stored at −80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... in Ca2+ and Mg2+ free medium (10% Krebs Solution (133 mM NaCl, 177 mM KCL, 1.75 mM NaH2PO4, all Merck), 1% MEM Non-Essential Amino Acids Solution (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... Afterward, the cells were washed twice in R10 media (RPMI-1640 with 10% FBS, 2 mM glutamine (G7513, Merck), 1% penicillin-streptomycin (P0781 ...
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bioRxiv - Cell Biology 2023Quote: ... The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck). The cells were incubated with the medium for 48 hours ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... the medium reservoirs were aspirated and 50 μL EGM-MV2 containing 100 ng/mL 10 kDa FITC-dextran (Merck) added to a single reservoir on each side of the device ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Cell Biology 2022Quote: ... MLg mouse lung fibroblasts (CCL-206, ATCC) were proliferation-inactivated by incubation in culture medium containing 10-μg·ml-1 mitomycin C (Merck) for 2 h ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed trice for 10 min with 0.05% Tergitol in TBS (Tris-Buffered Saline) and blocked for 1 hour with 2% BSA (Merck) in TBS ...
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bioRxiv - Genomics 2023Quote: ... DBCO-PEG4-conjugated antibodies were concentrated using an Amicon Ultra-0.5 NMWL 10-kDa centrifugal filter (Merck Milipore, UFC501024) and measured on a NanoDrop™ 2000 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Immunology 2023Quote: Isolated cells were transferred onto 18mm coverslips coated with 0.001% poly-L-lysine (Merck, cat: P4707-50ML, 10× stock) and incubated for 10 mins at 37°C to ensure cell attachment ...
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bioRxiv - Biochemistry 2023Quote: ... The chromatographic separation of anionic phospholipids was performed on a reverse phase C18 column (SUPELCOSIL™ ABZ PLUS; 10 cm x 2.1 mm, 3µm, Merck) using methanol:water (3:2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Plant Biology 2023Quote: ... The resin was pelleted as above and washed twice with 0.8 mL plant extraction buffer (see plant protein extraction section above) supplemented with 10 μM MG132 (Merck). Cold plant extract was either added directly to the resin (Supplementary Dataset S1 ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using a centrifugal filter unit (Amicon Ultra, 10 or 30 kDa cut-off, Merck), and the solvent was exchanged with 50 mM potassium phosphate buffer (pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... and then embedded in BMM resin (butyl methacrylate, methyl methacrylate, 0.5% benzoyl ethyl ether with 10 mM DTT (Merck)) at -20°C under UV light for polymerization ...
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bioRxiv - Plant Biology 2024Quote: ... each) onto a silica gel TLC plate (60 F254, 20 x 10 cm, 0.2 mm thickness; E. Merck, Germany) with the Linomat V sample applicator ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed by resuspension in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.2% IGEPAL® [Merck]) and pelleted again in the magnetic rack ...
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bioRxiv - Immunology 2024Quote: ... cells were plated on 10-well diagnostic microscope slides (Epredia) coated with 0.1% (w/v) poly-L-lysine (Merck) in H2O ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures not in active use were stored in liquid nitrogen in culture medium + 10% dimethyl sulfoxide (DMSO, Merck C6164).
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Sections or cells were pre-incubated in blocking buffer containing 2–10% normal donkey serum (Merck Millipore, Burlington, MA) and 0.1% Triton X-100 at RT for 1 h and incubated overnight at 4 °C with primary antibodies and subsequently incubated with secondary antibodies conjugated with Alexa Fluor 488 ...
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bioRxiv - Microbiology 2024Quote: ... Crude suspensions were washed 10 times with ice-cold sterile water and spores were purified with a HistoDenz (Merck) gradient 20-50%.
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bioRxiv - Microbiology 2024Quote: ... Labeled DiO - AEVs (1x1011/well [10 µl]) were added to THP1-b cell monolayers cultured on collagen solution (Merck) coated 12-well chamber slides (IBIDI ...
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bioRxiv - Plant Biology 2024Quote: ... 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 2% w/v PVPP, 10 mM DTT, 0.2% IGEPAL® [Merck]) supplemented with EDTA-free protease inhibitor tablets (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Plant Biology 2021Quote: ... A dilution series ranging from 1.25 μg ml−1 to 10 μg ml−1 bovine serum albumin (BSA, Merck KGaA) was used for calibration and processed together with the samples.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Biophysics 2021Quote: ... The solution was concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, MA, USA) and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences ...