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Citations for Merck :
1651 - 1700 of 2176 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was incubated with uTEV3 protease25(Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... animals were transferred to 3 cm NGM agar plates containing 5-Fluorouracil (5-FU) (10 µM) (Merck, #F6627) to prevent progeny production ...
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bioRxiv - Immunology 2024Quote: ... Tissue was stained for 1 h with a mixture of Hoechst-34580 DNA dye (10 µg/mL; Merck) and Phalloidin Alexa 647 conjugate (1:400 ...
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bioRxiv - Physiology 2024Quote: ... The non-adherent platelets were centrifuged on coverslips and blocked with 10% donkey serum (Merck KGaA, Darmstadt, Germany) and 1% bovine serum albumin (AppliChem ...
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bioRxiv - Plant Biology 2024Quote: ... using pre- treated silica gel plates (60 F254, 20 x 10 cm, 0.2 mm thickness; E. Merck, Germany). These TLC plates were modified by dipping in 0.2 M potassium dihydrogen orthophosphate solution (aqueous) ...
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bioRxiv - Developmental Biology 2024Quote: ... The beads were then washed three times for 5 min with 500 µl wash buffer (10 mM TRIS, 150 mM NaCl, 0.5 mM EDTA, 0.05% NP40 substitute (Merck); pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were lysed in 130 µl lysis buffer (10 mM TRIS, 150mM NaCl, 0.5 mM EDTA, 0.5% NP40 substitute (Merck); pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... after which they were washed three times in PBST and blocked overnight at 4°C in 10% serum (Merck) in PBS/0.3% TritonX100 ...
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bioRxiv - Microbiology 2021Quote: ... A 10 mL of the Shalgam sample was diluted with 90mL of Maximum Recovery Diluent (Merck, GmbH, Darmstadt, Germany) in Schott bottle and vortexed for 1 minute with highspeed vortex (MS-3 Basic ...
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bioRxiv - Physiology 2022Quote: ... the mixture was centrifuged for 10 min at 17,000 x g before transferring the mixture to an NMR tube (Merck) for subsequent NMR analysis.
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bioRxiv - Molecular Biology 2021Quote: ... The enzymes were concentrated to 10-19.5 mg ml−1 with Amicon Ultra 10k and 30k MWCO centrifugal filters (Merck), flash frozen in liquid nitrogen and stored directly at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 6 times for 10min with 1ml wash buffer (10mM HEPES ph 7.3, 150mM NaCl, MgCl2, 10% glycerol, 1% Empigen BB Detergent (Merck), 1x PI) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was used to transduce naïve Huh7 cells in the presence of 10 μg/ml polybrene (Merck Millipore). After 72 h ...
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bioRxiv - Biochemistry 2019Quote: ... Cas6 and Cas6 H47N were concentrated as required using an Amicon Ultra centrifugal filter (MWCO 10 kDa, Merck-Millipore).
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bioRxiv - Biophysics 2019Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then washed three times with PBS and coated with either 10 µg/ml fibronectin (Merck-Millipore, 341631) (U2OS and U-251 cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... vesiculosus fucoidan were separated by passing an aqueous fucoidan solution over a 10 kDa cut-off ultrafiltration membrane (Merck) followed by lyophilization.
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The elution peak fraction of both purified samples was collected and concentrated using Amicon Ultra-10 centrifugal filter (10,000 MWCO, Merck) by centrifugation at 4000 rcf for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Cell Biology 2021Quote: ... approximately 1x 106 cells of each cell line were spin-infected for 1 h at 1.9 × 103 g (3000 rpm) in the presence of 10 μg/mL polybrene (Merck) to facilitate the infection ...
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bioRxiv - Plant Biology 2021Quote: ... NaOCl and 0.02% (v/v) Triton X-100 for 10 min and washed five times with ultrapure water (Milli-Q; Merck). Following seed stratification at 4°C for 2 d ...
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bioRxiv - Microbiology 2021Quote: ... was eluted with solution-I and was concentrated to with an Amicon Ultra 10-kDa MWCO concentrator (Merck Millipore). The protein was further subjected to size exclusion chromatography on a HiLoad 16/600 Superdex 75 column pg column (Cytiva ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... transgenic worms were washed off NGM plates in M9 buffer and mounted on 2.5% agarose pads on glass microscopy slides and immobilised using 10 mM levamisole hydrochloride (Merck). Slides were then mounted onto a modified ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of cellular protein were resolved by 10 % SDS PAGE and were transferred to Immobilin P 0.45 μm PVDF membrane (Merck). Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mL of the supernatant were concentrated using an Amicon Ultra-15 100 kDa filter (regenerated cellulose; Merck/Millipore) by centrifuging at 4.000 × g for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Selected fractions were concentrated to 30 µl using 10 kDa cutoff centrifugal filters (Merck, Amicon Ultra 0.5ML 10K, #UFC5010). The samples were supplemented with 4x LDS (NuPAGE ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies were diluted with incubation buffer containing 10% (v/v) normal donkey serum (Merck KGaA, Darmstadt, Germany), 2% bovine serum albumin and 0.5% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... hsp70:R2nlsG double transgenic 5 dpf larvae were soaked in 10 µM 4-hydroxytamoxifen (4-OHT, Merck, Taufkirchen, Germany) or the corresponding amount of vehicle control ethanol for 10 h ...
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bioRxiv - Immunology 2021Quote: ... counted with a NC-250 cell counter (Chemometec, Denmark), and resuspended in heat-inactivated (h.i.) fetal bovine serum (Capricorn Scientific, Germany) containing 10% DMSO (Merck). Cells were immediately transferred into a -80°C freezer in a freezing container (Mr ...
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bioRxiv - Biophysics 2020Quote: ... and 25 µL of a 10 vol% solution of tetraethyl orthosilica (TEOS, reagent grade, 98%) in ethanol (absolute, Merck) 60 ...
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bioRxiv - Biophysics 2022Quote: ... the solution concentrated by ultrafiltration using Amicon Ultra centrifugal filters with a 10 kDa molecular weight cutoff (MWCO) (Merck) and passed over a Superdex 75 16/60 gel filtration column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... β2m and other contaminants using a C18 reversed-phase HPLC column (4.6 mm internal diameter x 10 cm, Chromolith Speed Rod, Merck) running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Biochemistry 2019Quote: ... all antibodies are buffer exchanged to 1x PBS (pH 7.4) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Developmental Biology 2021Quote: ... tropicalis females to be used for embryo collection were injected with 10 units of Chorulon HCG (Merck and Co.) 1-3 days before and 100 units of HCG in the morning of the embryo collection ...
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bioRxiv - Microbiology 2021Quote: ... USA) at a multiplicity of infection (MOI) of 5–10 in 1.5 mL Ex-Cell 405 medium (Merck, Germany), and the infected cells were incubated at 26 °C ...
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bioRxiv - Immunology 2020Quote: ... Collected proteins were buffer exchanged into PBS via multiple rounds of centrifugation in 10 kDA ultrafiltration tubes (Merck, USA). The purified protein content was measured by Bradford assay (B6916 ...
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bioRxiv - Microbiology 2020Quote: ... NO2- concentrations were determined using colorimetric test strips (NO2--test, 0-10 mgNO2-N·L-1, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Immunology 2021Quote: ... the protein content of the supernatants was concentrated 10x using centrifugal filters with 10 kDa cutoff (#UFC501096, Amicon, Merck). The concentrated samples were boiled in a reducing sample buffer and separated in 4-12% polyacrylamide gel (#NW04122BOX ...
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bioRxiv - Immunology 2021Quote: ... Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck), respectively ...
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bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were resuspended in 10% FCS-PBS again and fixed in 1% paraformaldehyde (PFA) for 10 min at room temperature and reaction quenched with 2.5M glycine (Merck) on ice and centrifuged at 400g for 8min ...
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bioRxiv - Microbiology 2022Quote: Nasal sIgA was obtained from 1 ml nasal swab eluates by centrifugation (1 minute, 10 000 xg) and supernatant was sterile-filtered through a 0.4 μm pore (Merck). We determined sIgA concentrations by ELISA (Human IgA ELISA kit ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes/embryos were placed in a 10 µl drop of culture medium covered with oil (oocytes: Mineral oil, Merck, M5310 ...
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bioRxiv - Bioengineering 2022Quote: ... were centrifuged at 5000 g for 10 min and the supernatants were filtered (22μm pore size Millex-GP Syringe Filter Unit, Merck) and frozen at −20°C for measuring medium composition ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with 0.1% Triton X-100 for 3 minutes and then blocked with 5 or 10% bovine serum albumin (BSA, Merck) in PBS for 20 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck, https://www.merckmillipore.com] containing 50 mM N-ethylmaleimide (NEM ...