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Citations for Merck :
1651 - 1700 of 5475 citations for 7H 1 3 Thiazino 2 3 i purin 5 1H one 2 3 8 9 tetrahydro 2 thioxo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and concentrated to 500 μl using Amicon Ultra-15 10K MWCO centrifugal filters (Merck Millipore UFC901008; final LDAO = 2%) and further purified by size-exclusion chromatography as described for human VDAC constructs but with 20 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... injections 3x a week (consecutive days) during 2 weeks of 25 mg/kg 3PO or 75 mg/kg 3PO or dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Merck, Overijse, Belgium) as control ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti phospho-ubiquitin (Ser65) (Merck Millipore, ABS1513-I, 1:1000), mouse anti GAPDH (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... supplemented with 50 μg ml-1 DNase I (Merck/Sigma–Aldrich) and 1 mg ml-1 lysozyme (Merck/Sigma–Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mg/mL DNAse I (Sigma Aldrich/Merck; Cat# 11284932001). The cell solution was passed through a 70 µm strainer and counted using Cellometer Auto T4 (Nexcelom Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... pSer403-p62/SQSTM1 (1:500) (Merck Millipore, Cat. no. MABC186-I), p62/SQSTM1 (1:1.000 ...
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bioRxiv - Molecular Biology 2022Quote: ... or E64d (20 µM, Merck, 88321-09-9). All inhibitors were used both prior to (1 h ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peak fractions containing the desired protein were pooled and concentrated to 2 ml in Amicon Ultra-15 concentrators 3,000 molecular weight cut-off (Merck Millipore, Carrigtwohill Co. Cork IRL). Concentrated protein was loaded onto a HiLoad 26/60 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were coated for 1h with 0.1 mg/ml poly-D-lysine (Merck) and then 3.5h with 0.018 mg/ml laminin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... mixed with ∼5 x 105 Jiyoye cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich/Merck) and spun at 800 g for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... anti-cortactin (p80/85, Merck, 05-180-I-25UL, 1:200 (IF)) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by further incubation with 1% DNase I (in saline) (10104159001, Merck) at room temperature for 5 min and removal of the supernatant ...
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bioRxiv - Neuroscience 2019Quote: ... samples were mixed with chilled buffer (50 mM Tris-HCl pH 9, 1% DOC, 50 mM NaF [Merck-Millipore] ...
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bioRxiv - Microbiology 2021Quote: ... The thin section was coated with 9-aminoacridine (Merck) by iMLayer (Shimadzu ...
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bioRxiv - Biophysics 2023Quote: Ammonium chloride (NH4Cl, Merck, cat. no. 12125-02-9). CAUTION ...
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bioRxiv - Biochemistry 2023Quote: ... Fifty μl of a suspension in water of GA-fixed cells were mixed with 50 μl of 2% osmium tetroxide in water (Cod. 75632-5ML, Merck Life Science S.r.l., Milan, Italy). After 30 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... samples were acidified to pH 2 with formic acid and desalted with pipette tips packed with six layers of C18 Empore™ SPE Disks (Merck, cat. no.66883-U) and dried in a Speed Vac at 45 °C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Rabbit Aquaporin 5 (Merck, 1:200), Rabbit ERG (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked 1h in 10% donkey serum in PBST and incubated overnight with primary antibodies for anti-GFAP (1:250, Merck Life Science, Hpa056030) and anti-MAP2 (1:200 ...
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bioRxiv - Immunology 2022Quote: Lungs were cut into 5 mm3 cubes and digested in 12 ml of collagenase I (2.4 mg/ml, Merck) and DNase I (1 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...
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bioRxiv - Microbiology 2021Quote: ... The suspension was treated with DNase I (1 μg ml-1) and RNase A (100 μg ml-1, Merck, India) to degrade nucleic acid residues of the host bacteria ...
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bioRxiv - Cancer Biology 2021Quote: ... 8) eosin (Merck. #1.15935.0100) for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mM MgCl2 (Merck), 40 mM EGTA (Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... 8 mM MgCl2 (Merck), 40 mM EGTA (Sigma)) ...
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bioRxiv - Cell Biology 2023Quote: ... CCK-8 (96992, Merck) was used according to the manufacturer’s instructions and measurements were performed at the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... 8 mM MgCl2 (Merck), 320 mM sucrose ...
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bioRxiv - Microbiology 2019Quote: ... and bacterial pellets were resuspended in 5 ml of cold column buffer with 1x PIC (Protease inhibitor cocktail set I; Cat. Nr. 539131-10VL, Merck) and 200 mM PMSF (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and DNase I (Merck). Bacterial lysis was performed by sonication (QSonica ...
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bioRxiv - Microbiology 2023Quote: ... DNase I (4716728001, Merck), S1 (N5661-50KU ...
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bioRxiv - Biochemistry 2024Quote: ... and DNAse I (Merck). After lysate clearing by centrifugation ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation for 5 minutes at 18 000 g the DNA pellet was washed twice with one mL 70% Ethanol (Merck Millipore) followed by 5 minutes centrifugation at 18 000g ...
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bioRxiv - Biochemistry 2023Quote: ... This mix was then deposited in sterilized 5 mm diameter plastic rings cut from PCR tubes (#683201, Greiner bio-one, Merck KGaA) on the surface of a chicken embryo chorioallantoic membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 1h in base assay medium (D5030, Sigma Aldrich, Merck, Darmstadt, Germany) supplemented with 2 mM glutamine ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
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bioRxiv - Cell Biology 2022Quote: ... the following ones from Merck: Anti-Actin AC-40 (A3853 ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30% ...