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Citations for Merck :
1451 - 1500 of 2050 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Microbiology 2022Quote: ... cells were cultured in 25 cm2 flasks at 37°C and 5% CO2 using advanced Dulbeccos modified Eagle medium (DMEM; TFS) with the addition of 10% fetal bovine serum (Merck), 50 U/mL penicillin and 50 μg/mL streptomycin (TFS) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
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bioRxiv - Microbiology 2022Quote: ... through six dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with 10 kDa molecular weight cutoff (Merck). For experiments studying the IgG-binding properties of Fc-III and Scr ...
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bioRxiv - Biophysics 2022Quote: ... The protein was dialyzed in 20 mM phosphate pH 7.4 and 500 mM NaCl to remove imidazole and concentrated using a 10 kDa Amicon® Ultra Centrifugal Filter (Merck).
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1% BSA/PBS and subjected to the Click-iT reaction in a solution containing 10 mM sodium ascorbate (Merck), 0.1 mM azide-PEG3-biotin conjugate (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... FOs were sonicated 2 x 10 sec in ice-cold 1:2 methanol/chloroform solvent containing SPLASH LIPIDOMIX MS standard internal standard mix (Merck). 1:6 H2O was added to the samples before shaking at 1000 rpm ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated for 10 minutes at 37°C with Liberase (250 μg/ml) and DNAse (50μg/ml Merck®). After incubation ...
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bioRxiv - Microbiology 2024Quote: ... the amiloride-sensitive component (amilmax) was determined by adding 10 µM amiloride to the apical compartment or ouabain (1 mM, # O3125, Merck) to the basolateral chamber to calculate the maximal ouabain-sensitive Na,K-ATPase activity (ouabmax).
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bioRxiv - Microbiology 2024Quote: ... 10 μM of BPP43_05035 was subjected to incubation with 0.5% porcine gastric mucin II or III (Merck, #M2378 and #M1778) in reactions conducted under pH 5.5-8.5 ...
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bioRxiv - Cell Biology 2024Quote: Approximately 1×106 trophozoites of the amoeba transformants were cultured in a 10-cm dish using BIS medium under a low oxygen environment provided by Anaerocult (Merck) and incubated at 35.5 °C for 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
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bioRxiv - Molecular Biology 2024Quote: ... the cells pellet was resuspended with a freezing medium composed of 90% KSR and 10% of Dimethyl Sulfoxide (DMSO, #D2438, Merck). Cell vials were stored in a liquid nitrogen tank.
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U mL−1 benzonase (Merck). After 1 h incubation at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... the culture medium of the cells was replaced at day 3 with differentiation medium containing 10 mM PEG950 (Merck; P3515).
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bioRxiv - Biochemistry 2024Quote: ... The purified solutions were concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, USA). For protein concentration determination ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT and 5%(v/v) glycerol by five cycles of filtration through 10 kDa Amicon filters (Merck Millipore), and finally concentrated to 0.5 mg/ml.
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Immunology 2024Quote: ... NK cells were gently transferred to a 15-mL conical tube (Falcon) containing 8 mL of 4°C RPMI 1640 (ATCC modification) medium supplemented with 10 U/mL DNaseI (Merck). The cryovial was rinsed with 1 mL of the RPMI medium to transfer the remaining NK cells to the 15-mL tube ...
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bioRxiv - Biochemistry 2024Quote: ... H2O was removed and ROS production was measured upon addition of a 100-μL assay solution which contained 10 μg/mL horseradish peroxidase (P6782, Merck), 10 mM luminol and the treatment (1 μM SCOOP ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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bioRxiv - Cell Biology 2023Quote: ... Conditioned media were collected after 48 h and centrifuged at 3,000 g for 10 minutes before passing through a 0.22 μm Millex® syringe filters (Merck Millipore). For viral infection ...
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bioRxiv - Neuroscience 2023Quote: ... coated dishes at low confluency (5,000–10,000 cells/cm2) in MEF-conditioned hES medium supplemented with 10 μM ROCK inhibitor (Y-27632; Merck, Cat#688000). On DIV0 of the differentiation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of gDNA from individual GCs (2 ng input) was performed with1 ul of JH reverse primer (10 uM, provided by MERCK) and 1.8 ul of LD forward primer set pools (10 uM per primer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Pathology 2023Quote: ... To prevent unspecific labelling the sections were incubated for 2 × 10 minutes in PB solution containing 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
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bioRxiv - Immunology 2023Quote: ... for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest) and 10% DMSO (Merck) using a cell freezing container (CoolCell LX ...
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bioRxiv - Immunology 2023Quote: ... the streptavidin tetramer mixture (containing D4, A1D3, A2D2, A3D1 and A4) was concentrated in an ultrafiltration cell (10 kDa cut off, Merck); mSav (A106C ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Amershan Hybond P PVDF membrane, Merck). Membranes were blocked in 5% milk in PBS/0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Preparative scale reactions were carried out in 3 mL Slide-A-Lyzer cassettes (10 kDa MWCO, Merck Millipore, Darmstadt, Germany) in combination with custom-made FM containers57 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells from identical conditions were collected and pooled in 10 ul in calcium-free and magnesium-free HBSS (Merck, H8264) + 50% FBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The cell suspensions were centrifuged at 10,000 x g for 10 minutes and bacterial pellets were lysed by addition of BugBuster Master Mix (Merck Millipore) for 25 minutes at room temperature with gentle agitation ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Immunology 2023Quote: ... Both the native fab and the deglycosylated fab were buffer exchanged into 150 mM ammonium acetate (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore). The samples were loaded into gold-coated borosilicate capillaries (in-house prepared ...
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bioRxiv - Cell Biology 2023Quote: Whole liver lysates and lipid droplet-enriched fraction proteins were loaded on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Merck). Membranes were incubated at room temperature for 1 h with blocking buffer (TBS ...
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bioRxiv - Biophysics 2022Quote: ... The eluted protein was buffer exchanged with PBS (pH 7.4) and was concentrated using 10 kDa cut off Amicon (Merck Millipore) concentrator to a final concentration of 1 mg/ml and stored at −80°C until further use.
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bioRxiv - Immunology 2023Quote: ... WT mice were 7-8 week-old Mice (C57BL/6 WT or Nr4a3-Timer-GS) were injected with 10 mg/kg azoxymethane (Merck) via i.p ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed in PBS and then transferred in a line on the glass chamber between electrodes filled with PBS containing 10 mM BrUTP (Merck). The poring pulse (voltage ...
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bioRxiv - Cell Biology 2023Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Pathology 2023Quote: ... lobus accessorius) were collected and fixed in 10 % neutral-buffered formalin (Chemie Vetrieb GmbH & Co) or 5 % glutaraldehyde (Merck KGaA) for microscopic and ultrastructural evaluation respectively.
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bioRxiv - Microbiology 2023Quote: ... 15 mL of the filtered culture supernatant was first concentrated using Amicon Ultra 15 mL Centrifugal Filters (cut-off 10 kDa, Merck), and the proteins were precipitated out of the solution using trichloroacetic acid (TCA ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated in a buffer containing 20 mM HEPES-KOH (pH 7.5) and 0.2% Noidet P-40 using a dialysis spin column (Amicon Ultra-10; Merck, Darmstadt, Germany). The concentrations of purified fluorescent proteins were measured using fluorescence correlation spectroscopy (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Once DRGs were removed and placed in ice-cold neuronal growth medium (Neurobasal medium, B27, and 10 ng/ml Nerve growth factor (NGF)-2.5 s (Merck Millipore), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 10 % (v/v) glycerol, 1% (w/v) digitonin (Carl Roth GmbH, Karlsruhe, Germany) and cOmplete protease inhibitor (Merck). Samples were rotated on a wheel for 1h ...
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bioRxiv - Biochemistry 2023Quote: ... tuberculosis GlnR-TAC were concentrated to 40 mg/mL using Amicon Ultra centrifugal filters (10 kDa MWCO, Merck Millipore, Inc.). Sequences of the above amtB oligonucleotides are shown in Supplementary Table S1.
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bioRxiv - Plant Biology 2023Quote: ... Sheared chromatin was incubated with 10 µg of α-H4.V or 5 µg of α-H4K5Ac (Merck 07-327) or 4 µg of α-H4 (Abcam ab10158 ...
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bioRxiv - Biophysics 2023Quote: Samples for native mass spectrometry were exchanged into 50 mM ammonium acetate (pH 6.8) using 10 kDa cut-off Amicon Ultra centrifugal filtration units (Merck Millipore). Samples were introduced to a first-generation Waters Synapt QToF (Waters Corporation ...
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bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...