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Citations for Merck :
1401 - 1450 of 2050 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were differentiated to macrophages by complementing the media with 10 ng/ml phorbol 12-myristate 13-acetate (#P8139, Merck) for seven days ...
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bioRxiv - Biophysics 2021Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 µL M2B-taxol (M2B buffer supplemented with 10 µM taxol (T7402, Merck)) ...
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bioRxiv - Cell Biology 2021Quote: ... The blocks were then washed with DDW 2 times for 10 min and incubated in 1% (w/v) uranyl acetate (Merck)/70% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: Urine samples were first adjusted to pH 8.0 using 500 mM ABC supplemented with EDTA-free protease inhibitor and then filtered through an Amicon Ultra-0.5 mL Centrifugal Filter Device (10 kDa, Merck Millipore). Collection of the retentate was performed by flipping the filter device upside-down and conducting centrifugation at 1,000g for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions that were highly enriched for Redβ177 were pooled and concentrated in a 10 kDa MWCO centrifugal concentrator (Merck Millipore) to a total volume of <2 ml ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Microbiology 2022Quote: Supernatants harvested from infected Rhileki cells were centrifuged at 3,500 rpm and precipitated with 10% polyethylene glycol 8000 (Merck, Germany) overnight in 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... was repeatedly washed with PBS supplemented with protease inhibitor cocktail and reconcentrated using an Amicon® Ultra-0.5 device with 10 K MWCO (Merck) until residual urea was diluted below 10 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM stock solution was prepared by dissolving ROCKi powder in 100% dimethyl sulfoxide (DMSO, Merck Life Science, UK, #D8418) and filtered through 0.22µM syringe filters ...
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bioRxiv - Developmental Biology 2021Quote: ... permeabilized for 30 minutes at room temperature in PBS supplemented with 0.2% Triton X-100 and 1% Bovine Serum Albumin (BSA) and blocked for 30 minutes at room temperature in 10% donkey serum (all from Merck). Samples were stained overnight at 4°C with the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were permeabilized with 60µL/well of 0.1% Triton X-100 for 10 minutes and stained with 50µL/well of DAPI (1:2000 dilution, in 1x PBST) (Merck, Germany) for 10 min [40] ...
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bioRxiv - Synthetic Biology 2022Quote: ... Dialyzed samples were further concentrated to 10 mg mL-1 using Amicon® Ultra-15 Centrifugal Filter Unit (Merck Millipore). The final concentration of glycerol was adjusted to 50% (v/v% ...
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bioRxiv - Microbiology 2020Quote: Stock solutions of crude extracts and fractions were prepared at a concentration of 100 mg/ml in 10% dimethyl sulfoxide (DMSO - Merck).
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bioRxiv - Microbiology 2019Quote: ... pH 7.4) through multiple dilution and centrifugation steps using Amicon Ultra-15 centrifugal filters (10 kDa cuttoff, Merck Millipore, Germany). Protein purity was estimated with SDS-PAGE and the concentration determined with the Pierce BCA assay kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing protein were pooled and concentrated using Amicon Ultra-15 centrifugal filter unit (10-kDa molecular mass cut-off; Merck) until total protein concentration of 10 mg·mL−1 was reached ...
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bioRxiv - Biophysics 2019Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated by using amicon 10 k MWCO centrifugal filtration devices (Merck).
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bioRxiv - Molecular Biology 2019Quote: ... and CM was concentrated to 1 ml at 4 °C using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... lipid extracts were also spotted on HPTLC precoated silica gel glass plates 60F254 (20×10 cm; 1.05642.0001; E. Merck, Darmstadt, Germany) using a Camag autosampler ATS4 sample applicator (Camag ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were then lysed in lysis buffer (50 mM KHPO4 pH 8, 100 mM NaCl, 10 mM Imidazole, 1X BugBuster (Merck), 25 U/ml Benzonase nuclease ...
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bioRxiv - Synthetic Biology 2019Quote: ... pH 8.5 (UA buffer) and loaded onto a 10 kDa molecular weight cut-off (MWCO) centrifugal filter (Merck Millipore, Australia) and centrifuged at 20,800 x g for 15 min at 22°C ...
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm: Merck) and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions 7-9 were individually collected (500 μL/fraction) and concentrated by Amicon Ultra-0.5 centrifugal filter unit with 10 kDa membrane (Merck Millipore) with 14,000 x g at 4 °C for 8 min centrifugation to have final volume less than 100 μL ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM EDTA) and glycerol (2.5%, v/v) were mixed and polymerized by the addition of fresh 10% APS solution (Merck Millipore) (final concentration 10% ...
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bioRxiv - Molecular Biology 2020Quote: ... GndHCl containing samples were subjected to a buffer exchange regime using Microcon-10 kDa centrifugal filter units (Merck, Darmstadt, Germany). Centrifugation (14’000x g ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed in 500 μl of lysis buffer (8 mL of cold PBS and 2 mL of 10% Triton X-114 supplemented with Halt Protease inhibitor (1:100) and Bezonase (Merck) (1:1000)) ...
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bioRxiv - Microbiology 2021Quote: ... Acid digestion was then performed for 3 h at 90°C placing the PP tubes on Teflon heating blocks after adding 2 mL hydrochloric acid and 1 mL nitric acid (10 M HCl and 14 M HNO3, respectively, both Suprapur, Merck) following protocol adapted from (62 ...
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bioRxiv - Microbiology 2019Quote: ... 100 μL of the faeces mixture was added to 10 mL of Selenite Broth (19 g/L selenite broth base, Merck, UK ...
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bioRxiv - Biophysics 2019Quote: ... 1 μM EDTA for PsaA) under oxygen-free conditions obtained utilizing an oxygen-scavenging system supplemented with 10 mM of Trolox (Merck)71 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% (vol/vol) paraformaldehyde for 10 min at RT and permeabilized with 0.3% Tween 20 (#822184, Merck Millipore). All the primary and secondary antibodies were diluted in blocking buffer consisting of 0.3 M glycine ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines WM35 and WM793B were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution (Merck, Darmstadt, Germany). For generation of conditioned melanoma media for immune cell inhibition experiments tryptophan (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... Gaithersburg, MD, USA) and 8-μm-pore polycarbonate membranes (Nucleopore, Pleasanton, CA, USA) coated with 10 μg/ml fibronectin (Merck) as described elsewhere (Prevete et al. ...
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bioRxiv - Microbiology 2019Quote: Standards of selected metabolites (Supplementary Table 1) were prepared at 10 µM in 80% acetonitrile (Hypergrade for LCMS LiChrosolv, Merck) and injected separately into a column connected to mass spectrometer interface ...
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bioRxiv - Immunology 2021Quote: All samples for CD-MS measurements were first buffer exchanged into 500 mM ammonium acetate solution (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore), unless otherwise stated ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... slides were blocked for 30 min in 0.3 % Triton / 10 % normal goat serum / TBS followed by overnight primary antibody incubation (1:1000; rb TH; Merck Millipore) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: Panc02 pancreatic tumours were flash frozen after excision and approximately 10 mg of tumour mass was homogeneised in RIPA lysis buffer (Merck) using a FastPrep-24 5G homogeneiser (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U/mL benzonase (Merck). After 1h incubation at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µl of sample were transferred into 100 µl of Amido-Black-Staining solution (0.25 % (w/v) Amido black 10 B (Merck, #1011670025)) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the final ribosome solution was diluted with 70S buffer 10-fold and then concentrated using Amicon Ultra (30 kDa cut, Merck) three times.
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of each sample was placed in an Amicon ultrafiltration centrifuge tube (Merck, 10 kD molecular weight cut-off), 120 μL reductant buffer (10 mM DTT ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were concentrated by centrifugation using spin columns (MW cut off of 10 kDa) (cat number UFC501096– Merck Millipore). Protein concentration was estimated by UV using calculated extinction coefficients (42,400 mol/l-1.cm-1) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Immunology 2022Quote: For protein crystallization “empty” HLA-DPA1*02:01-DPB1*01:01 was purified on a Superdex 75 10/300 GL column (Merck) into 20mM Tris pH 8.0 ...