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Citations for Merck :
1401 - 1450 of 1874 citations for 5 FLUORO 2 MERCAPTOBENZOTHIAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... thawed pellet was resuspended into 20 ml lysis buffer (0.025 M Tris pH 8; 0.5 M NaCl; 2 mM MgCl2; 100 U/ml Benzonase (Merck); 0.25 mg/ml lysozyme (Roche) ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the compounds were tested by use of a MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] assay (Merck) at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... cells pre-labeled with transferrin-Alexa Fluor 594 were treated for 2 hours with 10 μM SAR405 (Merck, cat ...
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bioRxiv - Microbiology 2022Quote: ... concentrated to 2-10 mg/ml using Amicon concentration devices (Merck, 3,000 or 10,000 Da cut-off filter) and stored in aliquots at −80 °C until needed ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times in 0.1 M sodium cacodylate buffer pH 7.2 ± 0.1 containing 0.2 M sucrose and 2 mM MgCl2 (Merck Millipore), and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2 × 104 cells were cultured in an 8-well chamber slide (Merck Millipore, Massachusetts, USA) and then fixed with 4% paraformaldehyde in PBS buffer for 15 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human HepaRG hepatoma cells (1.5-2×105 cells/cm2, Biopredic International, Rennes, France) were cultured in William’s E medium (Merck) supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Cell Biology 2024Quote: ... L-glutamine and antibiotic/antimycotics and decidualized with 1µM PGE2 or 1µM relaxin-2 (accession # P04090) (both Biotechne Ltd, Abbington, U.K.) in combination with 1µM medroxyprogesterone acetate (MPA) (Merck) for up to 4 days ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were inoculated with 100,000 SB28-GFP cells suspended in 2 µl of DMEM (Merck, Darmstadt, Germany) (glioblastoma mice ...
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bioRxiv - Molecular Biology 2023Quote: Cells of 2-3 days growth with approximately 70-90% confluency have been treated with Bortezomib (Merck, #504314) at 100 nM for 42 h ...
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bioRxiv - Microbiology 2023Quote: ... auris cells (CHRIST Alpha 1-2 LD plus lyophilizer, Osterode, Germany) using Tri Reagent (Merck Ltd. Budapest, Hungary). The quality of RNA was determined using the Eukaryotic Total RNA Nano kit (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped with 2 µl of 0.5 M EDTA and separated using thin layer chromatography plates (Merck) with 0.3 M LiCl and 0.3 M formic acid as the mobile phase ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
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bioRxiv - Microbiology 2023Quote: ... A fraction of the inoculum was directly transferred into 2 ml tube containing TRI reagent (Sigma-Aldrich, Merck) and silica beads (0.1 mm diameter ...
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bioRxiv - Biophysics 2023Quote: ... 5 and 10 μl of the lipid extract 2 cm from the bottom on aluminum-backed silica gel 60 TLC plates (cat#: 1.05553.0001; Merck) together with DOPG ...
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bioRxiv - Molecular Biology 2022Quote: ... eggs rafts were hatched in trays containing 2 L of Milli-Q water (Synergy® UV, Merck, Germany). Larvae were fed continuously until the pupae stage with TetraMin® baby fish food (Tetra ...
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bioRxiv - Zoology 2024Quote: ... The sample was then placed in 2 ml petroleum ether (boiling range 40-60°C, Merck, Darmstadt, Germany) at room temperature for two days ...
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bioRxiv - Biochemistry 2023Quote: The reaction mixtures (0.5, 1 or 2 µL) were spotted onto TLC Silica Gel 60 F254 plates (Merck). As for analysis of cyclization activity ...
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bioRxiv - Cancer Biology 2024Quote: ... to assess migration the lower faces of the inserts were coated with 2 μg/ml fibronectin (Merck, F0895). Subsequently ...
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bioRxiv - Systems Biology 2024Quote: ... with 200 µl of a 2% w/v solution of sodium fluorescein in 0.9 % saline (Merck Ltd., UK). Tracer was permitted to circulate for 1 h before animals were deeply anaesthetised with sodium pentobarbital (50 mg/kg ...
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bioRxiv - Synthetic Biology 2021Quote: ... Seed cultures from glycerol stocks were inoculated 20 hours overnight in 5 mL Invitrogen Luria Broth Base LB medium (ThermoFischer, USA) and supplemented with Kanamycin sulfate (Merck, Germany) (50 μg/mL) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS for 1 hour and probed with mouse γ/9d 2G10.2 (1:1000, MERCK MABT1335), rabbit anti mouse IgG (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then fixed with 4% p-formaldehyde (PFA) during 15 min and stained with DAPI (5 min, 1 μg/ml in PBS; Merck-Millipore).
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bioRxiv - Microbiology 2019Quote: ... We dissolved dried lipid extracts in 40 μL of 1:1 methanol:CHCl3 and used 5-10 μL of sample for thin layer chromatography (TLC) on TLC silica gel 60 plates (Merck, Germany) with a mixture of 65:25:10 CHCl3:CH3OH:CH3COOH for the mobile phase ...
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bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and Thecadinium kofoidii were harvested during late exponential growth phase by filtration onto 5 µm SMWP Millipore membrane filters (Merck, DE) and washed off with sterile seawater ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
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bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Bioengineering 2021Quote: ... INS-1 cells in bioprinted grid scaffolds were stained with thiazolyl blue tetrazolium bromide (MTT) after 5 days in culture according to the manufacturer’s protocol (Merck, Darmstadt, Germany). Proliferation was determined using an ATP-based assay with luminescent readout (CellTiter-Glo® 3D Cell Viability Assay ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 15 minutes in 95% and 100% ethanol and 5 minutes in hexamethyldisilazane (HMDS, Sigma-Aldrich, Merck, St Louis, USA). Samples were left to air-dry overnight in a biosafety cabinet prior to mounting on electron microscope stubs covered with carbon tape ...
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bioRxiv - Microbiology 2021Quote: ... The diluted solution was concentrated to approximately 5 mL using 30 kDa molecular weight cutoff Amicon Ultra centrifugal filters (Merck KGaA), passed through a Millex-GP syringe filter with 0.22 μm pore size (Merck KGaA) ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Microbiology 2020Quote: ... column using a nanoUPLC (nanoLC Ultra 1D plus, Eksigent, USA) and eluted (400 nL/min) with acetonitrile gradient (5%-80%) (LiChrosolv®, Merck) with 0.1% formic acid ...
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bioRxiv - Immunology 2020Quote: ... The pseudo-particle-containing supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore).
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bioRxiv - Biochemistry 2021Quote: ... 5’-GCCCAAAGAATCAGAACAGATGC-3’) or the genomic 18S ribosome gene (mouse 18S forward: 5’-AAACGGCTACCACATCCAAG-3’, mouse 18S reverse: CAATTACAGGGCCTCGAAAG-3’) (Merck KGaA). Primers specific for mtDNA gives rise to a 201bp product ...
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bioRxiv - Microbiology 2020Quote: ... the HA-GlmS line was transfected with the pHGBrHrBl-1/2 construct in order to episomally express cytosolic GFP then selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). To generate Pfcerli2SLI-TGD ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and SsuT3-oE-R (5′-AGTCAG GGATCC CTA CAC CAC CTT CAC TTT GGT ACC) with KOD Hot Start DNA polymerase (Merck Millipore) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore). HEK-293 cells stably expressing hACE2 were seeded into 0.1% gelatin-coated 96-well plates (Greiner ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation for 5 minutes at 18 000 g the DNA pellet was washed twice with one mL 70% Ethanol (Merck Millipore) followed by 5 minutes centrifugation at 18 000g ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Molecular Biology 2019Quote: ... samples were immersed in xylene (ten successive immersions for 5 minutes each) and mounted on slides cover-slipped using Entellan mounting medium (Merck, Millipore). Images were acquired using on Axio microscope with 20x/0.35 and 40x/0.55 Zeiss A-Plan objectives (Carl Zeiss ...
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bioRxiv - Immunology 2019Quote: ... and viral titers were determined in Vero cells using a standard plaque assay at day 5 post-infection with crystal violet staining (Merck Millipore). The viral titers were determined in aliquots of harvested medium ...
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bioRxiv - Systems Biology 2020Quote: TGF-β/Activin/Nodal signal inhibitor (SB) stock (4–5 mM): SB 431542 hydrate (#S4317-5MG, Merck & Co., Inc., NJ, USA) diluted in DMSO (#D2650-5×5ML ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...