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Citations for Merck :
1351 - 1400 of 1874 citations for 5 FLUORO 2 MERCAPTOBENZOTHIAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 cells were plated per well and were further cultured in the presence or absence of 5 µM oligomycin A (Merck) at 37 °C in a humidified chamber containing 5% CO2 and 1% O2 for 2 hours ...
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bioRxiv - Biophysics 2024Quote: ... and the elution fraction was concentrated to 5 ml using an Amicon Ultra Centrifugal Filter Unit (30 kDa MWCO, Merck). The concentrated protein was applied to size exclusion chromatography using a HiLoad 16/600 Superdex 200 pg (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies (table 1) were added in 5% milk TBS-T and membranes were developed using ECL luminol kit (Merck) and chemiluminescence films (Amersham Hyperfilm ECL ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... the sections were equilibrated to room temperature and then washed twice for 5 minutes (min) in PBST (0.1% Triton X-100 (Merck, #108603)/PBS) ...
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bioRxiv - Immunology 2023Quote: ... peptides were dissolved at a concentration of 1 to 5 mg ml-1 in 1x PBS and incubated with TCEP agarose CL4-B (Merck) for 1 hour to reduce paired cysteines ...
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bioRxiv - Plant Biology 2023Quote: ... Sheared chromatin was incubated with 10 µg of α-H4.V or 5 µg of α-H4K5Ac (Merck 07-327) or 4 µg of α-H4 (Abcam ab10158 ...
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bioRxiv - Immunology 2023Quote: ... Titers were determined by transduction of Jurkat cells (ACC 282; DSMZ) in presence of 5 µg/ml Polybrene Infection/Transfection Reagent (Merck). After 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... equipped with a LiChrospher® 100 RP-18 (125 mm x 4 mm, 5 µm) C18 reversed-phase column (Merck). The elution solvents consisted of ultrapure water with 0.1% formic acid (A ...
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bioRxiv - Immunology 2023Quote: ... were transduced with respective CORA receptors in a multiplicities of infections (MOI) of 1 and 5 μg/mL Polybrene (Merck). Cells harboring the receptor were enriched by using biotinylated anti-EGFR antibody and anti-biotin microbeads (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: CORA receptors were transduced into a previously described Jurkat-based reporter cell line (12) by addition of lentiviral particles in a MOI of 1 and 5 μg/mL Polybrene (Merck) using spinoculation ...
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bioRxiv - Pathology 2023Quote: ... Then sections were blocked for 45 min using a blocking buffer containing 5% goat or donkey serum (depending on the host species of the secondary antibody) (#G9023 and #D9663, Merck) and 0.3M glycine (#56-40-6 ...
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bioRxiv - Biophysics 2022Quote: ... C-CaM was cloned using PCR amplification of the C-terminus of WT-CaM with added flanks of a 5’ NdeI overhang and a 3’ BamHI overhang and ligated into pET21a vector (Merck). Insertion of PCR product into pET21a was achieved with standard protocols (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... All the oocytes harvested from COCs were transferred to activation medium composed of KSOM medium supplemented with 5 mM strontium chloride (13909, Merck) and 2 mM EGTA (A0878 ...
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bioRxiv - Pathology 2023Quote: ... lobus accessorius) were collected and fixed in 10 % neutral-buffered formalin (Chemie Vetrieb GmbH & Co) or 5 % glutaraldehyde (Merck KGaA) for microscopic and ultrastructural evaluation respectively.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight with an anti-GFP rabbit antibody (0.5 μg/ml) (Tamamaki et al., 2000) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000; MAB3120, Merck Millipore). After a rinse ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were caught and rinsed in 1x PBS (3 × 15 min) prior to incubation in blocking solution (5% NDS (Merck) in 0.3% PBS-Triton-X-100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peak fractions were pooled and concentrated to 5 μM (0.7 mg/mL) final concentration using an Amicon Ultra-15 (Merck Millipore) for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Microbiology 2023Quote: Samples separation with chromatography was performed on the SeQuant ZIC-HILIC column (5 μm, 200 Å, 150x2.1 mm, Merck, Germany) with a flow rate of 0.6 mL/min ...
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bioRxiv - Microbiology 2023Quote: ... column 5 µm 150 x 4.6 mm with a 20 x 2.1 mm ZIC-pHILIC guard column (both Merck Millipore, Australia) (25 °C) ...
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bioRxiv - Biochemistry 2023Quote: ... Briefly this involved separation of metabolites using a ZIC pHILIC column (150 mm × 4.6 mm, 5 μm column, Merck Sequant) before entering an Orbitrap Q-Exactive (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... A SeQuant® ZIC®-HILIC 5 μm particle size analytical column 150 x 4.6 mm (Merck KGaA, Darmstadt, Germany) and a Acquity UPLC BEH C18 column 1.7 μm particle sized analytical column 2.1 × 100 mm connected to an Acquity UPLC BEH C18 VanGuard Pre-column ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... anti-total-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor (anti-tAMPAR) (Cat.#AB1504, Merck Millipore, Burlington, MA, USA), anti-phospho (Ser845)-AMPAR (anti-pAMPAR;cat.#AB5849 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The interlocking ring included a central odorant delivery platform comprising a 10-mm-diameter cover glass and two 5-mm-diameter filter discs (WHA10016508; Merck) for the evaluation of VOCs (stl files are provided in Extended Data Fig ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Biophysics 2024Quote: ... HeLa cells were cultivated at 37 °C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Molecular Biology 2024Quote: ... Milli-Q water suitable for LC-MS analysis was freshly obtained from Millipore Milli-Q® Advantage A10 system (18,2 MΩ×cm, <5 ppb) equipped with a LC-Pak® Polisher filter unit (#LCPAK0001) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Biochemistry 2024Quote: ... for 5-10 min at room temperature to reveal bound cytokine and reaction was blocked by adding HCl 2N (Merck) solution (20 μL/well) ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... PAM (0.1 mg/mL in DMSO) or DMSO were incubated with LCAT (5 μg/mL in assay buffer + 60 μM albumin (Merck)) 1:100 v/v at 37°C gently shaken ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 5-10 μg of primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) in 250 μL LB3 with 1% Triton X-100 overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was centrifuged (3215 g for 5 min) and then filtered (Merck Millex™ Syringe Filter, PES, 0.22 μm). In the enrichment phase ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded (2×104 cells/well) in 24-well plates in alphaMEM medium (Merck, Darmstadt, Germany, #F0925) plus 10 % FCS (Gibco ...
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bioRxiv - Genomics 2019Quote: The populations were enriched in metaphasic cells by 2 h of treatment with 200 nM nocodazole (Merck, M1404) prior to cell recovery ...
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bioRxiv - Plant Biology 2021Quote: 2-AA-labeled N-glycans prepared for HILIC-HPLC were desalted on C18 ZipTips (Millipore, Merck, Warszawa, Poland), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). The Deep Vent exo- DNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) clone 3E10 (Merck, 04-1571-I, 1:5,000), and anti-RNA polymerase II CTD repeat YSPTSPS antibody clone 8WG16 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 2 twice with PBS and were permeabilized with PBS–0.06%-Triton X-100 (Merck, USA) for 10 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). Deep Vent exo-DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: AKT1/2 phosphorylation was inhibited with InSolution™ Akt Inhibitor VIII (1-10 µM, Merck KGaA, Darmstadt, Germany) in FCS-free medium for 3 hours prior to treatment with HGF (10 ng/µl).
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bioRxiv - Systems Biology 2022Quote: ... 70 kDa FITC-dextran and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Merck Life Science UK Ltd. ...
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bioRxiv - Biophysics 2022Quote: Purified FHL was concentrated to 1-2 mg/mL using an Amicon centrifugal concentrator (100-kDa cutoff; Merck) and 3 µL of protein solution was applied to freshly glow-discharged C-flat 1.2/1.3 or 2/1 holey carbon grids (Protochips) ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Prior to sterilization pH of ASW was calibrated at 8.5 ± 0.5 through the addition of 2 M NaOH (Merck) solution ...
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bioRxiv - Physiology 2019Quote: Mouse intestine was fixed with 2.5% glutaraldehyde (Agar Scientific, Stansted, Essex, UK) and 2% paraformaldehyde (Merck, Darmstadt, Germany) in 0.1M Na-cacodylate-buffer (Merck ...
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bioRxiv - Physiology 2020Quote: ... following which they were incubated with anti-MAP-2 antibody (AB5543, 1:500, Merck Millipore, Burlington, MA, USA) in blocking buffer overnight at 4℃ ...