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Citations for Merck :
1251 - 1300 of 1342 citations for PI 3 Kinase p110 delta Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4 °C with a monoclonal anti-RBPMS antibody (1:500, Rabbit, ABN1362, Merck Millipore) for the retina ...
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bioRxiv - Physiology 2020Quote: ... rabbit polyclonal (RP) against phosphorylated Tyr 1077 ObRb (pTyr1077ObR; 1:500, cat# 07-1317, Merck Millipore, Burlington, Vermont, USA), GP against phosphorylated Tyr 1138 ObRb (pTyr1138ObRb ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunoassays: primary antibodies - rabbit polyclonal anti-RTN3 (#ABN1723, Merck Millipore; 1:400 dilution), monoclonal anti-NF200 (#N5389 ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used were rabbit anti-CB1R (1:1,000, Immunogenes) and mouse anti-actin (1:50,000, MAB1501, Merck Millipore). Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate ...
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bioRxiv - Cell Biology 2021Quote: ... Immuno-reactive complexes were detected using horseradish-peroxidase coupled anti-rabbit or anti-mouse antibodies (Sigma-Aldrich/Merck, Germany) and subsequent detection of chemiluminescence signals with a ChemoCam Camera system (INTAS Science instruments GmbH ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were incubated in the antibody solution (Anti-NG2 Chondroitin Sulfate Proteoglycan, Rabbit, 1:250, AB5320, Merck Millipore) for 48 h at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and incubated overnight in the same solution with the primary antibody to CB1R (1:1,000, rabbit, Immunogenes) and neuronal nuclei (NeuN) (1:1,000, mouse, MAB377, Merck Millipore), at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Neuroscience 2022Quote: ... the second step consisted of incubating overnight in PBS-Triton with rabbit anti-Aldh1a1 (1:500, HPA002123, Sigma Merck) and goat anti-calbindin (1:500 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... tyrosine-hydroxylase (TH) was detected with the primary antibody anti-TH in rabbit (1:600, EMD Millipore - Merck, USA). After washing in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA was performed using Duolink In Situ Red Starter kit (Mouse/Rabbit) according to the manufacturer protocol (Merck, DUO92101).
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bioRxiv - Immunology 2023Quote: ... and nitrotyrosine levels were immune labelled using immunostaining with a rabbit polyclonal anti-nitrotyrosine antibody (06-284; Merck Millipore) and detected using an Alexa Fluor–conjugated secondary antibody (Invitrogen Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Knockout of the molecules was confirmed by SDS-PAGE and western blotting using rabbit anti-ADAM10 polyclonal (Merck AB19026) at 1/500 followed by goat anti-rabbit HRP (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... The HRP-Conjugated secondary antibodies((Anti-Rabbit (raised in goat) and anti-Mouse (raised in goat)) were from Merck. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Microbiology 2022Quote: ... the supernatants were concentrated 50-fold using Amicon Ultra-4 centrifugal filters with a molecular weight cut-off of 3 kDa (Merck-Millipore, Burlington, MA, USA). Samples were mixed with 3× Laemmli loading buffer and heated for 3 min at 85 °C before SDS-PAGE analysis ...
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bioRxiv - Microbiology 2020Quote: ... spores were collected from 5-day-old cultures NO3 medium (0.17% yeast nitrogen base, 3% sucrose, 100mM KNO3) by filtering through miracloth (Merck; pore size of 22–25μm). Spores were centrifuged ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HPLC purification of the (S,S)-MLN-4760 was based on the comparison with the commercially available compound (MLN-4760; MW: 428.31; Merck, CAS N° 305335-31-3). The isolation of the desired (S,S)-diastereoisomers of the respective fluorinated MLN-4760 derivatives was based on the assumption that the HPLC elution sequence of the diastereoisomers would be identical to that of the MLN-4760 lead compound.
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bioRxiv - Plant Biology 2024Quote: ... 2-(1H-Indol-3-yl)-4-oxo-4-phenyl-butyric acid (PEO-IAA; OlChemIm, Olomouc, Czech Republic) and indole-3-carbinol (I3C; Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Immunology 2024Quote: ... the wellbeing of the fish was followed at least once a day and fish exhibiting symptoms, signs of pain or discomfort were euthanised with an overdose of a Tricaine anaesthetic (Ethyl 3-aminobenzoate methanesulfonate, (Merck, Kenilworth, New Jersey, USA)) ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Cell Biology 2019Quote: ... 500µl of this pellet suspension was added to a tube in which 2.5 mg of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, Avanti Polar lipids, Merck, Sigma-Aldrich, l’Isle d’Abeau, France) in chloroform had previously been evaporated ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Immunology 2024Quote: ... we included αTIM-3+ αPD-1 and used anti-human RSV-IgG4 as an isotype control antibody (5 μg/mL, 60AGK S228P, Merck & Co., Inc., Rahway, NJ, USA). On day six ...
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bioRxiv - Cell Biology 2020Quote: ... product T7451; used at 1/500) and CHMP1B (rabbit polyclonal antibody, ATLAS product HPA061997; used at 1/500) were from Merck; CHMP2A (rabbit polyclonal ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by incubation with a secondary Cy3-labeled polyclonal goat anti-rabbit antibody (AP132C, 1:800, Merck Millipore, Burlington, USA). Cells were mounted on slides using Fluoroshield™ with DAPI mounting medium (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were detected with HRP-conjugated anti-rabbit or anti-mouse IgG and visualised with Immobilon Western HRP substrate (Merck).
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bioRxiv - Neuroscience 2019Quote: ... A background block incubation in PBS with 1% TritonX with 10% Donkey serum was conducted before retinas were incubated in primary antibody solution (PBS with 1% Triton-X with 2.5% donkey serum with 1:250 dilution of rabbit anti-SWS cone opsin antibody, AB5407 Merck Millipore) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Physiology 2020Quote: ... blocked in 5% NGS and 0.5% Triton diluted in PBS and incubated over night at 4°C with primary antibody: rabbit anti-NG2 (1/50, AB5320, Merck), goat anti-PDGFRα (1/200 ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK), HIF2A (Novusbio/NB100-122, Secondary Anti-rabbit IgG-HRP Cell Signalling/70745, London, UK) and GAPDH (Merck/MAB374 ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibody incubation was performed at room temperature for 90 min utilizing a horseradish-peroxidase (HRP)-labeled anti-rabbit IgG (Merck), and staining was visualized with DAB (Dako ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by washing and staining with horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG secondary antibodies (1:5,000 dilution; Merck Millipore), respectively ...
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bioRxiv - Immunology 2021Quote: ... plates were fixed with 1:1 acetone and methanol Anti-S1 rabbit polyclonal antibody (Novus) was added to each well and incubated for 1Hr followed by incubation with FITC labelled anti-rabbit antibody (Merck). Fluorescence images were captured using an inverted microscope (ZeissAX10).
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse Cy3 conjugated secondary antibody (cat no. AP124C) and anti-rabbit Cy3 conjugated secondary antibody (cat no. AP132C) were procured from Merck. Anti-rabbit Alexa Fluor 488-conjugated secondary antibody (cat ...
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bioRxiv - Molecular Biology 2024Quote: ... chromatin samples cross-linked by formalin were enzymatically shared and incubated with rabbit anti-AcH4 antibody or isotype control antibody (Merck) at 4 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA probe anti-mouse PLUS and anti-rabbit MINUS (Merck). Incubation with all antibodies was accomplished in a humidified chamber for 1 h at 37 °C ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody anti-Rabbit or/and goat IgG antibody conjugated with HRP (GENA934, AP106P, Merck) at room temperature for 1 hour for enhanced chemiluminescence ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Plant Biology 2024Quote: ... Secondary incubation was completed with a 1:40 dilution of Goat anti-Rabbit IgG 10 nm gold conjugate for a further hour (Merck).
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...