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Citations for Merck :
1151 - 1200 of 1342 citations for PI 3 Kinase p110 delta Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: J774A.1 macrophages with or without infection (3 hour) were lysed in a specific lysis buffer containing DCP-Bio1 (Merck # NS1226) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting sample of holo-SmLPMO10A was concentrated to ~ 150 μM using an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck) and the sample was split into three equal volumes of ~ 500 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was removed (around 25 ml) and either concentrated 10-fold with Amicon 3 kDa spin columns (Merck, cat. #UFC900308) and brought to a final volume of 2 ml for subsequent Western blot analysis (Fig.1B ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Plant Biology 2023Quote: ... the dried pellets were redissolved in 80 % methanol (1µL/mg) and 3 µL were analysed by HPTLC on a Silica Gel 60 plate (Merck, Darmstadt, Germany). The mobile phase used was ethyl acetate ...
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bioRxiv - Molecular Biology 2023Quote: ... and dialyzed against cold sterile PBS using an Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa MWCO (Merck Millipore). The resulting product was then passed through a 0.22 µM filter.
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bioRxiv - Molecular Biology 2023Quote: ... Eluted fractions were concentrated and dialyzed against PBS using Amicon Ultra-15 Centrifugal Filter Units with a 3 kDa MWCO (Merck Millipore). Endotoxins were removed using High Capacity Endotoxin Removal Spin Columns (Pierce) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... were homogenized (60 Hz, 90 s) and extracted by ethanol three times (95%, 3 × 300 mL, vortex 10 min each, Merck, Germany). The three batches of extracts were pooled together and centrifuged for 20 min at 10000g and 4°C (Bruker ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Evolutionary Biology 2023Quote: Total RNA was extracted from ∼150 injected animals (3 days old planulae) with the aid of Tri- Reagent® (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... AT2 Organoids were fixed with PFA for 3 minutes and then released from matrigel by incubating the culture with Corning Cell Recovery Solution (Merck, CLS354253) for 2 hours at 4 °C.
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were probed with a secondary antibody: either anti-rabbit-HRP (1:5,000; Merck Millipore AP132P), anti-mouse-HRP (1:5,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse or anti-rabbit IgG coupled with Alexa-555 (A-21422, Invitrogen, and AP510C, Merck, respectively) were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_2190413), mouse anti-SV2C (MABN367, RRID: AB_2905667) and rabbit anti-TH (AB152, RRID: AB_390204) from Merck Millipore ...
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bioRxiv - Genetics 2019Quote: ... rabbit anti-TbRBP16 [1:1,000] (27) and mouse anti-EF1a [1:200,000] (clone CBP-KK1, Merck-Millipore) for 18 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1p was detected using a rabbit anti-phospho H4/H2A S1p antibody (07-179; Upstate/Merck-Millipore) at a dilution of 1:4000 ...
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bioRxiv - Neuroscience 2020Quote: The following primary antibodies were used: rabbit polyclonal anti-MuSK (1:1,000; Merck Millipore, catalog no. ABS549), anti-HA.11 (1:100 for immunoprecipitation ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-Ascl2 (8F1, MAB4417) and rabbit anti-Sox9 (AB5535) and p57 (06556) were purchased from Merck Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... Post-mating, the female rabbit was injected intravenously luteinising hormone (dose 25 i.u., Luveris (75 IE, Merck)) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:600-diluted rabbit anti-CENP-C (#554),55 mouse anti-HP1α (1:1,000, #MAB3584, Merck Millipore), rabbit anti-HP1β (1:800 ...
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bioRxiv - Molecular Biology 2024Quote: PLA was performed by using Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Merck, DUO92101-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Microbiology 2023Quote: ... Goat Anti-Rabbit IgG HRP conjugate (632131/Merck/Massachusetts, USA, 1:10,000 were procured from Merck (USA). Mouse ß-Actin (3700S/ 1:1000/Cell Signalling technology/Massachusetts ...
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bioRxiv - Plant Biology 2024Quote: ... before and after incubation of blots with appropriate secondary antibody (anti-rabbit HRP (Merck #A0545; 1:50,000); anti-mouse HRP (Merck #A9044 ...
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bioRxiv - Neuroscience 2024Quote: ... or α-tubulin (1:1000; 12G10) and rabbit polyclonal antibodies against detyrosinated tubulin (1:10000, AB3021, Merck) and 3-NT (1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... secondary antibodies goat anti-rabbit IgG and goat anti-mouse IgG conjugated with Atto 594 (Merck; 77671) and Abberior® STAR RED (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... and an alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin G secondary antibody (Sigma-Aldrich, Merck, Barcelona, Spain), both at 1:1000 dilution.
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bioRxiv - Cancer Biology 2024Quote: ... and secondary antibody anti-rabbit (CST, 1:3000) diluted in PBS containing 4% skim-milk powder (Merck) and 0.2% Tween 20 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... The final protein preparations were concentrated to 1 mM using Amicon® Ultra 10 or 3 kDa cut off centrifugal filters (Merck KGaA). Aliquots were frozen in liquid nitrogen and stored in −80 °C.
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: The vessel lumen was washed 3 times with PBS ++ (PBS with 1mM CaCl2, 0.5mM MgCl2) and fixed with 4% paraformaldehyde (PFA, Merck, #30525-89-4) at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... and 10% ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Biochemistry 2022Quote: ... [2H7]22R-Hydroxycholest-4-en-3-one ([2H7]22R- HCO) was prepared from [2H7]22R-HC by treatment with cholesterol oxidase enzyme (Streptomyces sp., Merck, Dorset, UK) (11).
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bioRxiv - Microbiology 2021Quote: ... and 10 % Ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...