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Citations for Merck :
1251 - 1300 of 4636 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Amicon Ultra-4 centrifugal filters with 100 kDa molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 μM Nalgene® syringe filter units (sterile ...
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bioRxiv - Plant Biology 2023Quote: ... while 4-chloro-7-nitrobenzoxadiazole (NBD-Cl, 97%, Sigma-Aldrich, Merck Group, St Louis, MO, USA) was applied at 270LgLha−1 of active ingredient ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested DIP material was clarified (3000 × g, 10 mins and 4 °C) and sucrose (Merck, #84097) was added at a final concentration of 4 % ...
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bioRxiv - Cell Biology 2023Quote: The eluate was concentrated using an Amicon Ultra-4 concentrator (Merck Millipore, 10 kDa cut-off), and incubated overnight at 4°C with TEV and 3C proteases to remove the C-terminal StrepTagII and C-terminal 6xHis tag from the HAUS2 and HAUS1 subunits respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Amicon Ultra-4 centrifugal filters with 100,000 Da molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 µM Nalgene® syringe filter units (sterile ...
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bioRxiv - Microbiology 2023Quote: SDS gel electrophoresis was performed using pre-cast mPAGE™ 4-20% bis-tris gels (Merck) and 20 µl of PS extracts were mixed in Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... cells were fixed at room temperature either with 4% paraformaldehyde (D1408, Merck Life Science, Milan, Italy) solution in DPBS or with methanol (32215 ...
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bioRxiv - Microbiology 2024Quote: ... Centrifugal units Amicon® Ultra-4 3KDa molecular weight cut-off (MWCO) were obtained from Merck KGaA (Darmstadt ...
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bioRxiv - Microbiology 2024Quote: The cells in 96-well plates were fixed with 4% paraformaldehyde (PFA, Merck, Cat. no. P6148) in phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... 50.000 cells plated on coverslips were fixed for 15 min with 4% PFA (MC1040051000, Merck, Germany). Blocking was performed in 10% normal goat serum (G6767 ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were concentrated at 3,500 rpm and 4 °C using Amicon Ultra centrifugal filter units (Merck) or using a stirred cell ultrafiltration device ...
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bioRxiv - Biochemistry 2024Quote: ... The NuMA-containing fractions were pooled and concentrated (Amicon Ultra 4, 100 kDa MWCO [Merck Millipore]). The Strep-tag II was cleaved off by incubating with TEV protease for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and mounted on slides using Mowiol-based mounting medium (12% Mowiol 4-88 (Merck, 81381-250G), 30% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... d were separated on mPAGE™ 4-12% Bis-Tris Precast Gels (Merck, MP41G10 or MP41G12) through standard SDS-PAGE protocol ...
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bioRxiv - Biochemistry 2024Quote: ... coli C41 DE3 cells were used to inoculate 4 ml of overnight express terrific broth (Merck) with 50 µg/ml Kanamycin in 24 well plate format ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatants were subjected to end-on rotation at 4 °C with anti-FLAG (Merck, F1804) that was pre-conjugated to Protein A/G beads (Pierce ...
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bioRxiv - Developmental Biology 2024Quote: ... the sample were incubated in ethyl cinnamate for 4 hours at room temperature (112372-100G, Merck).
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bioRxiv - Immunology 2021Quote: ... 1 μM Src inhibitor-1 (Merck), 2.5 μM Src inhibitor PP1 (Cayman Chemical ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µM Ferrostatin-1 (Merck, #SML0583), 10 µM oleic acid (Merck ...
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bioRxiv - Microbiology 2023Quote: ... 1 g.L-1 L-cysteine (Merck) and 1 g.L-1 NaHCO3 (Carl Roth GmbH ...
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bioRxiv - Microbiology 2021Quote: ... MilliQ water was produced in-house using a Milli-Q Integral 3 (LC-Pak polisher, Millipak express 40 filters, 0.22 µm, Merck) for a final organic content < 3ppb ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked for 3 hr in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl, PH 8.3, both Merck) plus 0.05% Tween-20 (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Biochemistry 2021Quote: ... 2/3 of the resulting supernatant (RIPA-insoluble fraction) was filtered through a nitrocellulose membrane (0.2 μM pore size, Merck) using a filter trap slot blot (Hoefer Scientific Instruments) ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed 3 times with deionized water (Milli-Q® IQ 7003 Ultrapure Lab Water System, Merck), and dehydrated by washing them in a graded series of ethanol ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μL of each sample was injected into a Chromolith FastGradient RP 18-e column (50 x 3 x 2 mm; monolithic silica with bimodal pore structure, macropores with 1.6mm diameter; Merck) protected by a precolumn (Gemini NX RP18 ...
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bioRxiv - Physiology 2022Quote: ... Metabolites were chromatographically separated in a SeQuant ZIC-cHILIC column (PEEK 100 x 2.1 mm, 3 μm particle size; Merck) at 30°C using a method consisting of a gradient running at 0.25 mL/min from 100% mobile phase B (9:1 acetonitrile:water containing 5 mM ammonium acetate pH 6.8 ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... This was followed by concentration with a centrifugal concentrator (Amicon Ultra-15 3 k device, Merck Millipore, Burlington, MA), and after supplementing with 10 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v) ACN (Merck, USA) containing 0.1% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was applied to a centrifugation filter (Amicon Ultra 0.5 mL 3 K device; Merck KGaA, Darmstadt, Germany), and the fraction containing free amino acids was obtained by centrifugation (15,000 × g ...
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bioRxiv - Biophysics 2021Quote: ... the dry lipid film was rehydrated with 3 mL of 0.2 M 8.5 pH Bicine (Merck Life Science, Norway) buffer (pH adjusted with NaOH (Merck Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with 0.1% Triton X-100 for 3 minutes and then blocked with 5 or 10% bovine serum albumin (BSA, Merck) in PBS for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... with 3 mg/mL of bovine serum albumin (BSA) and 100 µM of IBMX (Sigma-Aldrich, Merck, Darmstadt, Germany), and collected ...
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bioRxiv - Microbiology 2022Quote: ... and 10% ultra-pure water from Direct-Q 3 UV Water Purification System with LC-Pak Polisher (Merck KGaA) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Molecular Biology 2022Quote: All samples were centrifuged at 16,000 × g for 30 min and 400 µL supernatant was loaded on a 0.5 mL centrifugal ultrafiltration unit with 3 kDa cut-off membrane (Amicon, Merck) and concentrated down to 20 µL ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 3 hours in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl, PH 8.3, both Merck) plus 0.05% Tween-20 (TBST ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the presence or absence of aphidicolin 2μM for 3 hours together with cytochalasin B (3μg/ml, Merck Millipore) to block cytokinesis prior to fixation for immunofluorescence.
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bioRxiv - Biochemistry 2022Quote: ... The LE11-27 DNA was dialyzed against the same buffer and concentrated to ~ 2.5 mM (Vivaspin 500, 3 kDa MWCO, Merck). The complex was formed by mixing the protein and the DNA in a 2:1 protein-to-DNA ratio (final concentration of 800 µM and 400 µM ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Merck 200-664-3). None of the cell lines were authenticated but low passage numbers were utilized.
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Japan) with a Discovery HS F5 column (2.1 mm i.d. × 150 mm, 3 μm particle size, Merck, Darmstadt, Germany) coupled with a Q Exactive instrument (PFPP-LC/MS ...
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bioRxiv - Cancer Biology 2024Quote: ... each coverslip was washed with PBS 3 times each for 5 minutes and nuclei were stained with Hoechst (Merck Sigma ...
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bioRxiv - Microbiology 2024Quote: Infected RBCs were lysed with saponin (0.15%) in PBS and washed 3 times with protease inhibitor cocktail solution (PI, cOmplete Mini; Merck). Proteins were extracted using 1% Triton-X 100 (w/v ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with Roti-Block (Carl Roth, Karlsruhe, Germany) or 3% bovine serum albumin (Merck KGaA, Darmstadt, Germany) in TRIS-TWEEN-buffered saline and incubated overnight with the primary antibodies as indicated ...