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Citations for Merck :
1151 - 1200 of 1660 citations for Recombinant Mouse PVRL2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore) with a membrane molecular weight cut off 10 kDa.
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore; Merck).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration using an Amicon Ultra-0.5 mL (10 kDa cutoff, Merck Millipore) at 14,000 × g and 4°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were then sterilised by passing the eluate through a 0.2 μm filter (Merck Millipore), then concentrated using a 3kDa Vivaspin 20 centrifugal concentrator column (Sartorius ...
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bioRxiv - Immunology 2023Quote: ... Medium was collected after this period and filtered through 0.45μm ultra-low protein binding filter (Merck Millipore) to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore ...
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bioRxiv - Biophysics 2023Quote: ... The TEV-cleaved protein was then buffer exchanged using a 30 kDa MWCO centrifugal filter (Amicon, Merck) into buffer containing no imidazole and 50 mM KCl (25 mM K·HEPES ...
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bioRxiv - Systems Biology 2023Quote: ... The separated proteins were transferred in a semi-dry system onto PVDF membranes (Immobilon P, Merck Millipore) using transfer buffer (192 mM glycine ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 centrifugal filters (100 kDa cut-off, Merck-Millipore). After treatment with 1 mM diamide for 15 min to completely oxidize the protein ...
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bioRxiv - Molecular Biology 2023Quote: ... PsC23 protein-containing fraction was collected and imidazole was removed by 10K centrifugal filter (Merck Millipore, Germany) using buffer (20 mM Tris-Cl ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by reducing Tris-glycine SDS-PAGE and transferred to polyvinylidene difluoride (PVDF, Merck Millipore) membranes followed by enhanced chemiluminescence analysis (Advansta ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE gels were used for protein separation followed by transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal quantities of protein were separated on SDS-PAGE gels and transferred to the PVDF membranes (Merck), which were then blocked with 1 % nonfat skim milk in TBS-T for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a Immobilion®-P Transfer Membrane (0.45 µm pore size, Cat#: IPVH00010, Merck) and blocked for 1h at RT in 1xTris Buffered Saline Tween (TBST ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-PAGE and then transferred to 0.45 μm PVDF membrane (IPVH00010, Merck Millipore). To keep the amounts of protein equally ...
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bioRxiv - Biochemistry 2023Quote: His-tagged proteins were labeled using Ni-NTA-ATTO 550 or Ni-NTA-ATTO 647 from Merck Group ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Microbiology 2024Quote: ... Target protein-containing fractions were combined and concentrated using an Amicon Ultra-4 50 kDa filter (Merck) operated at 7,500 x g and 4 °C ...
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bioRxiv - Physiology 2023Quote: ... 20 μg of total protein were transferred onto centrifugal filter units (Microcon-30 kDa; Merck, Darmstadt, Germany) and washed with a buffer containing 8 M urea and 100 mM Tris (pH 8.5) ...
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bioRxiv - Plant Biology 2024Quote: ... and proteins were concentrated using a 30 kDa Amicon Ultra-4 Centrifugal Filter Unit (Merck Milipore; www.merckmillipore.com). Protein concentration was determined employing the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The fractions with target proteins were pooled and concentrated using an Amicon Ultra 3K NMWL (Merck Millipore). The sample was ultracentrifuged (45,000 rpm x 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... GST was labeled with mouse Anti-GST Tag monoclonal antibody (05-782, Merck Chemicals; 2.5 μg/ml). To account for variations in the amount of fusion protein ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study were: mouse monoclonal antibodies against Centrin (1:500 for immunofluorescence; Merck Millipore catalogue number 04-1624 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse or anti-rabbit IgG coupled with Alexa-555 (A-21422, Invitrogen, and AP510C, Merck, respectively) were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... RRID: AB_2190413), mouse anti-SV2C (MABN367, RRID: AB_2905667) and rabbit anti-TH (AB152, RRID: AB_390204) from Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... and one of the following primary antibodies: mouse anti-GAD67 for staining GABAergic cells (Merck & Co., MAB5406), goat anti-Substance P for staining D1-containing cells (Santa Cruz ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: monoclonal mouse anti-puromycin (clone 12D10, MABE343, Merck Millipore; 1:1000), polyclonal rabbit anti-Tau (T6402 ...
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bioRxiv - Genetics 2019Quote: ... rabbit anti-TbRBP16 [1:1,000] (27) and mouse anti-EF1a [1:200,000] (clone CBP-KK1, Merck-Millipore) for 18 h at 4°C ...
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bioRxiv - Physiology 2019Quote: ... using the Milliplex mouse metabolic hormone magnetic bead panel (MMHMAG-44K, Merck Millipore, Fontenay sous Bois, France). Circulating cytokine levels were analysed using the 10-spot V-plex kit Proinflammatory Panel 1 Mouse (Meso Scale Discovery ...
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bioRxiv - Cell Biology 2021Quote: ELISA was performed to detect serum GH (rat/mouse growth hormone ELISA kit, Merck KGaA, Dermstadt, Germany), IGF-1 (mouse/rat IGF-1 ELISA kit ...
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bioRxiv - Cell Biology 2020Quote: ... c-Src was detected using anti-Src (GD11 clone) antibody (1:200, Merck Millipore, Mouse, 05-184). Phosphorylated c-Src was assessed using anti-phospho-Src Tyr 418 antibody (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-Ascl2 (8F1, MAB4417) and rabbit anti-Sox9 (AB5535) and p57 (06556) were purchased from Merck Millipore ...
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bioRxiv - Genomics 2022Quote: ... firstly with anti-DIG2 (sheep anti-mouse Ig digoxigenin conjugate, 1:200, Merck Millipore, Billerica, MA, USA) and antistreptavidin (1:25 ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used for Western blotting were Goat-Anti-mouse IgG HRP conjugated (catalog #12-349; Merck) and Goat-Anti-rabbit IgG HRP conjugated (catalog #12-348 ...
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bioRxiv - Cell Biology 2022Quote: ... 1:600-diluted rabbit anti-CENP-C (#554),55 mouse anti-HP1α (1:1,000, #MAB3584, Merck Millipore), rabbit anti-HP1β (1:800 ...
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bioRxiv - Neuroscience 2023Quote: ... NBP1-92693AF647) and the oligodendrocyte transcription factor OLIG2 (Anti-OLIG2 clone 211F1.1 mouse mAb, Merck Millipore, MABN50A4). Samples were kept on ice throughout the isolation and staining procedure ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with primary antibodies against puromycin (mouse monoclonal 1:500, Merck), Par3 (rabbit polyclonal 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with an anti-puromycin antibody (mouse monoclonal 1:500, Merck) combined with an anti-β-actin antibody (rabbit polyclonal 1:1000 ...