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Citations for Merck :
1001 - 1050 of 1660 citations for Recombinant Mouse PVRL2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... L1s were then broken up to extract proteins using a Beadbug homogenizer (Merck Life Science) at the highest setting for 90 seconds three times at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Molecular Biology 2022Quote: The concentration of protein extracts was determined by BCA assay kit with BSA standards (Merck).
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein bands were visualised using a chemiluminescence substrate (Immobilon Forte, Merck Life Science UK Ltd.) on the ChemiDoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K, 0.5 ml, Merck) and tryptically digested over night following the FASP (Filter-Aided Sample Preparation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Genetics 2023Quote: ... Anti-S6 Ribosomal Protein Antibody (used with 1:1000 dilution) was purchased from Merck (#ZRB1172). α-tubulin antibody (used with 1:5000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Immunology 2023Quote: ... EVA71 VP0/VP2 reactive proteins were detected using anti-EVA71 VP2 antibody clone 979 (Merck) at 1:2000 dilution and anti-mouse IgG HRP conjugate ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously123 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Merck). The membranes were blocked for 30 min with Blocking-One or Blocking-One P (Nacalai Tesque ...
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bioRxiv - Physiology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously107 ...
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bioRxiv - Immunology 2023Quote: ... Protein bands were visualized by treating the membrane with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were transferred into an Amicon Ultra Centrifugal filter device (10 kDa MWCO, Merck) for buffer exchange [50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Physiology 2023Quote: The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously23 ...
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bioRxiv - Biochemistry 2024Quote: ... Glycerol-free eluted protein was concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (Merck) at 10,000 x g ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was filtered through an Ultrafree-MC centrifugal device with hydrophilic PTFE membrane (Merck) at 10,000 x g ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were then desalted and concentrated by ultrafiltration with Amicon Ultra 0.5 10K (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... In brief supernatant protein concentration was standardised prior to being concentrated using Microcon® (Merck) 10Kda filter columns and centrifugation (14,000g 20 minutes) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved via SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Merck, GE10600023) with a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff, Merck) and exchanged with NMR buffer D (10 mM sodium phosphate ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin (100 μg) was precleared with protein A agarose/Salmon sperm DNA beads (Merck) and 2 % taken as input ...
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bioRxiv - Neuroscience 2020Quote: ... Then they were incubated in mouse anti-Cx35/36 antibody (MAB3045, EMD Millipore, Merck, USA) at a dilution of 1:250 in blocking solution for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with anti-mouse antibody (1:5,000; Merck Millipore, Germany; Cat. No. AP124P). After incubation ...
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bioRxiv - Developmental Biology 2021Quote: PLA was performed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Merck, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: The procedures for Cre-recombinase (anti-Cre-recombinase antibody; 1:500, mouse, MAB3120, Merck Millipore), and Fos-B (anti-FosB antibody 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... using the following antibodies: mouse anti-NeuN (1:1500, Cat. # MAB377, Merck KGaA, Darmstadt, Germany), chick anti-GFP (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used were the following: mouse anti-NeuN (1:500, RRID: AB_2298772, Merck Millipore) or rabbit anti-RFP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were mouse monoclonal antibodies against actin (1:10000, Merck Millipore, MAB1501R), synaptophysin (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... and the other for neuronal nuclei (mouse anti-NeuN monoclonal antibody (Merck MAB377, 1:200)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1/5000 dilution of anti-mouse IgG conjugated with peroxidase (POD; Merck, MO, USA) was used to reveal the presence of bound anti-E-tag ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal pS129 (11A5, 1:10,000) and tyrosine hydroxylase (TH, #AB152, Merck Millipore, 1:1000). Appropriate secondary Alexa Flour antibodies (Life Technologies and Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with anti-mouse or rabbit secondary antibody conjugated to HRP (Merck AP181P, AP132P) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Mouse monoclonal antibodies used in this study were anti-β-actin (AC-15, Merck, A1978), anti-His tag (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass (Merck). After treatments cells were washed 3x with PBS for 10 minutes ...
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bioRxiv - Physiology 2023Quote: ... mouse monoclonal anti-phosphoserine conjugated to Duolink® PLA MINUS probe (DUO87012, 1:50, Merck).
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bioRxiv - Molecular Biology 2023Quote: ... and Cy3-conjugated goat anti-mouse secondary antibody (diluted at 1:200, #AP124C; Merck Millipore) for detection ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti vinculin (V9131) and rabbit polyclonal anti beta actin (A2066) were from Merck. Rabbit polyclonal antibodies to ARL8b (A88749) ...
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bioRxiv - Developmental Biology 2024Quote: ... and LH were measured using the Mouse Pituitary Magnetic Bead Panel (MPTMAG-49K, Merck, USA) according to manufacturer instructions ...