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Citations for Merck :
1151 - 1200 of 3874 citations for 1R 2S 6R 7S 4 4 DIMETHYL 3 5 DIOXA 8 AZATRICYCLO 5.2.1.0 2 6 DECAN 9 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... a blocker of the sarco/endoplasmic reticulum Ca2+-ATPase pump (SERCA) and Carbonyl cyanide 4- (trifluoromethoxy) phenylhydrazone (FCCP, Merck-Sigma-Aldrich), a protonophoric uncoupler of mitochondrial oxidative phosphorylation that depolarizes the mitochondrial membrane and leads to the organelle’s release of calcium ...
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bioRxiv - Cell Biology 2021Quote: ... 25 min) and alkylated (20 mM iodoacetamide, 30 min, room temperature) and samples concentrated with Amicon Ultra-4 columns (Merck-Millipore), After protein precipitation with methanol/chloroform ...
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bioRxiv - Microbiology 2020Quote: Feces were hydrated in 500 µL sterile Milli-Q water overnight at 4 °C and then mixed with 500 µL of absolute ethanol (Merck, USA) for 60 min at RT ...
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bioRxiv - Plant Biology 2020Quote: ... on a LiChroCART® column (LiChrospher 100 RP-18, 125 x 4 mm, 5.0 μm particle size, Merck KGaA, Darmstadt, Germany), with constant flow rate of 1 mL min-1 of 100% solvent A (acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... To perform this, tissues were fixed over a filter paper imbibed with 30% sucrose (Winkler, Chile) in PBS–4% paraformaldehyde (Merck, USA) for at least 15 min ...
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bioRxiv - Plant Biology 2021Quote: ... Individual carotenoids were quantified in a Shimadzu HPLC (LC-10AT) with a diode array detector using a RP-18 Lichrocart 125-4 reverse phase column (Merck®) and a mobile phase composed of acetonitrile:methanol:isopropanol (85:10:5 v/v) ...
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bioRxiv - Systems Biology 2021Quote: ... Excess FSBA reagent was removed by ultracentrifugation with 15 mL 10 K MWCO Amicon® Ultra-4 centrifugal filter units (Merck) at 3,500 × g at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... and EV-containing fractions were pooled and concentrated with a 10 kDa molecular weight cut-off Amicon Ultra-4 centrifugal filter unit (Merck Millipore).
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Cell Biology 2022Quote: KELLY cells were grown on 20 x 14 cm dishes and treated for 14-16 hours with 100 µM of 4-Thiouridine (Merck, T4509). Cells (1.6 × 108 ...
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bioRxiv - Neuroscience 2022Quote: ... was either boiled with 4x SDS sample buffer (4% SDS, 40% glycerol, 20% ß-Mercaptoethanol in 250 mM Tris-HCl, Merck) at 95°C ...
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bioRxiv - Biophysics 2022Quote: ... for 10 min prior to fixation after washing out puromycin were fixed with 4% PAF for 30 min at 37°C and subjected to immunoblotting using puromycin antibody (Merck, MABE343). For the negative control ...
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bioRxiv - Cancer Biology 2022Quote: IACS-010759 (from the Institute for Applied Cancer Science at MD Anderson)15 and 4-hydroxytamoxifen (Merck Life Science, Darmstadt, Germany) were dissolved in DMSO and ethanol ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... enzymes were concentrated by centrifugation in Centriprep® 30K and Amicon® Ultra-4 Ultracel®-10K devices (Merck Millipore Ltd). Purity of enzymes was assessed by SDS–polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and covalently cross-linked the brain to the agarose by incubating overnight at 4 °C in 0.5 – 1 % sodium borohydride (NaBH4, Merck KGaA, Germany) in 0.05 M sodium borate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions were concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore, Merck, Burlington MA, USA) and purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE LS ...
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bioRxiv - Neuroscience 2022Quote: ... To fix the cells they were washed once with warm PBS and incubated for 20 min at 37°C with 4 % paraformaldehyde (Merck Millipore). After washing ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 10 % glycerol) using an Amicon® Ultra-4 regenerated cellulose NMWL 10 kDa centrifugal filter unit (Merck). The protein was then stored as 50 μl aliquots at −80°C ...
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bioRxiv - Plant Biology 2021Quote: ... were moved to new tubes and concentrated at 14,000×g for 10 min at 4 °C using Amicon ultra-0.5 ml centrifugal filters (catalog number Z677094, Merck millipore, Sweden). Thylakoid ...
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bioRxiv - Immunology 2020Quote: ... samples were centrifuged at 22000 x g for 20 minutes at 4 °C and the supernatant was filtered through Millex GV 220 nm filter (Merck-Millipore). For acid treatment acetic acid was used with 0.1 N final concentration ...
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bioRxiv - Neuroscience 2021Quote: ... fixed with a 4% PFA solution and stained for tyrosine hydroxylase (TH; rabbit anti-TH, 1:1000, Cat#: 657012, Merck Millipore) and neurobiotin (Streptavidin Alexa Fluor conjugate 647 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell suspension was centrifuged for 3 min at 200 x g and pellets resuspended in 1% BSA in PBS by pipetting 4 times up and down and filtering through 40 μm Flowmi strainer (Merck, Germany). Apoptotic and duplet cells were removed by staining the cell suspension with propidium iodide (Invitrogen ...
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bioRxiv - Pathology 2023Quote: ... blocked with normal serum for 1 h at 22–24°C and incubated overnight at 4°C with rabbit polyclonal anti-Lubricin antibody (1:500, MABT401, MERCK, DEU). The sections were then washed three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were incubated overnight on a rocker at 4 °C with appropriate combinations of the following antibodies: guinea pig anti-RBPMS (1:300, catalog no. ABN1376; Merck Millipore); goat anti-Brn3a (1:100 ...
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... quality of the elution was assessed by loading 50µl of the resulting elution on a pre-cast 4-15% gradient gel (Merk, MP41G15) and incubated with SYPRO RUBY (Merck, S4942) protein gel stain ON at RT ...
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bioRxiv - Biophysics 2023Quote: ... and fractions containing histone octamers were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). The protein concentration was determined with Bio-Rad Protein Assay Dye Reagent (Bio-Rad # 5000006).
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted at 460 g at 4 °C for 30 s and concentrated in a 1% GDN pre-equilibrated 30 kDa Amicon Ultra centrifugal filter (Merck Millipore). The concentrated eluate was spun through a Zeba Spin Desalting Column 40 kDa MWCO (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and covalently cross-linked the brain to the agarose by incubating overnight at 4 °C in 0.5 – 1 % sodium borohydride (NaBH4, Merck KGaA, Germany) in 0.05 M sodium borate buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing CLPB3 were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resuspended in 80% methanol-water and a volume of 4 μL was injected on a SeQuant ZIC/pHILIC Polymeric column (Merck Millipore). The separation of metabolites was achieved at 25°C with a flow rate of 0.20 ml/min ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were isolated from lungs and a tissue reference piece was separated and fixed in 4% paraformaldehyde (MERCK, Kenilwork, NJ, USA) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were next supplemented with 100 µl of 4 mM methoxypolyethylene glycol maleimide (PEG-maleimide) (5000; Merck, cat. No. 63187) in the SDS buffer ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... Seeds were surface-sterilized by 70% ethanol and washed 4 times with sterilized ultra-pure water (Milli-Q water, Merck-Millipore) followed by stratification at 4 ºC for 2 d ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
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bioRxiv - Bioengineering 2023Quote: ... filtered through 0.45 µm syringe filters (for Cas9/sgRNA delivery experiments) and concentrated using 100kDa MWCO Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore) to volumes of 100-250 µL.
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bioRxiv - Neuroscience 2023Quote: ... rats were anesthetized and underwent transcardial perfusion with 100 mL of saline followed by 100 mL of 4% paraformaldehyde (Merck, Germany) at 72 h following ICH induction ...
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bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...