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Citations for Merck :
1101 - 1150 of 1715 citations for Recombinant Mouse EGFL6 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff, Merck) and exchanged with NMR buffer D (10 mM sodium phosphate ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin (100 μg) was precleared with protein A agarose/Salmon sperm DNA beads (Merck) and 2 % taken as input ...
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bioRxiv - Neuroscience 2020Quote: ... Then they were incubated in mouse anti-Cx35/36 antibody (MAB3045, EMD Millipore, Merck, USA) at a dilution of 1:250 in blocking solution for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with anti-mouse antibody (1:5,000; Merck Millipore, Germany; Cat. No. AP124P). After incubation ...
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bioRxiv - Developmental Biology 2021Quote: PLA was performed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Merck, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: The procedures for Cre-recombinase (anti-Cre-recombinase antibody; 1:500, mouse, MAB3120, Merck Millipore), and Fos-B (anti-FosB antibody 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... using the following antibodies: mouse anti-NeuN (1:1500, Cat. # MAB377, Merck KGaA, Darmstadt, Germany), chick anti-GFP (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used were the following: mouse anti-NeuN (1:500, RRID: AB_2298772, Merck Millipore) or rabbit anti-RFP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were mouse monoclonal antibodies against actin (1:10000, Merck Millipore, MAB1501R), synaptophysin (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... and the other for neuronal nuclei (mouse anti-NeuN monoclonal antibody (Merck MAB377, 1:200)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1/5000 dilution of anti-mouse IgG conjugated with peroxidase (POD; Merck, MO, USA) was used to reveal the presence of bound anti-E-tag ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal pS129 (11A5, 1:10,000) and tyrosine hydroxylase (TH, #AB152, Merck Millipore, 1:1000). Appropriate secondary Alexa Flour antibodies (Life Technologies and Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with anti-mouse or rabbit secondary antibody conjugated to HRP (Merck AP181P, AP132P) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Mouse monoclonal antibodies used in this study were anti-β-actin (AC-15, Merck, A1978), anti-His tag (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass (Merck). After treatments cells were washed 3x with PBS for 10 minutes ...
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bioRxiv - Physiology 2023Quote: ... mouse monoclonal anti-phosphoserine conjugated to Duolink® PLA MINUS probe (DUO87012, 1:50, Merck).
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bioRxiv - Molecular Biology 2023Quote: ... and Cy3-conjugated goat anti-mouse secondary antibody (diluted at 1:200, #AP124C; Merck Millipore) for detection ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti vinculin (V9131) and rabbit polyclonal anti beta actin (A2066) were from Merck. Rabbit polyclonal antibodies to ARL8b (A88749) ...
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bioRxiv - Developmental Biology 2024Quote: ... and LH were measured using the Mouse Pituitary Magnetic Bead Panel (MPTMAG-49K, Merck, USA) according to manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The pure proteins were concentrated using a 3000-high molecular weight cutoff centrifugal filter (Merck-Millipore). The concentrated proteins were then subjected to size-exclusion chromatography using a Superdex-75 GL column (GE healthcare pre-equilibrated with 25 mM Tris-HCl 6.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF (Merck KGaA, Darmstadt, Germany)) stain was used as a loading control and imaged prior to blocking ...
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bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was collected and protein concentration was determined using the Direct Detect® Spectrometer (Merck Millipore). The proteins (30 µg ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were separated by SDS-PAGE and transferred onto PVDF (polyvinylidene fluoride) membranes (Merck Millipore, DE). The membranes were then blocked for 1 h in 5% non-fat milk in PBST and incubated with CD64 and CD16 antibodies and β-actin antibody respectively overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were transferred and western blotted using anti phospho-T187 p27KIP1 antibody (Merck, 06-996). Band intensity was revealed using ECL reagent (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... downward capillary action was used to transfer protein to immune-blot PVDF membrane (Merck cat# IPVH00010). Membranes were blocked with 5% milk in wash buffer (0.05% Tween in PBS ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... Equal protein amounts were separated by SDS PAGE and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Neuroscience 2021Quote: ... Purified protein was concentrated to 8 mg/mL using 30 kDa cut-off AMICON concentrators (Merck).
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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bioRxiv - Biochemistry 2019Quote: All proteins were expressed in E.coli Rosetta-2 DE3 cells (EMD Millipore, Merck KGaA, Darmstadt, Germany). Cells were transformed via heat shock (42°C for 35 secs ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was concentrated to ~65 mM using a MWCO 30,000 Amicon Ultra Centrifugal Filter (Merck), flash-frozen and stored at −80 °C until use.
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bioRxiv - Microbiology 2020Quote: Viral proteins were separated by SDS-PAGE and transferred to polyvinylidene disulfide (PVDF) membranes (Merck Millipore). After blocking the membranes with 5% skim milk ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were pelleted by centrifugation and lysed in Bugbuster™ protein extraction reagent (Merck; Darmstadt, Germany) by continuous agitation for 20 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Cell Biology 2021Quote: ... Purified proteins were were concentrated > 60 μM using Amicon Ultra centrifugal filters (Merck; MWCO 3 kDa), rebuffered to 50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The collected protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut off and frozen at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... Protein samples for mass spectrometry were purified and concentrated using ZipTipC-18 (Merck, Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2021Quote: ... Protein signals on the membranes were detected with Immobilon Forte Western HRP substrate (Merck, Darmstadt, Germany) and the ChemiImager Ready (Alpha Innotec,San Leandro USA).
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...