Labshake search
Citations for Merck :
1351 - 1400 of 1852 citations for Recombinant Mouse EGFL6 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2019Quote: ... using the Milliplex mouse metabolic hormone magnetic bead panel (MMHMAG-44K, Merck Millipore, Fontenay sous Bois, France). Circulating cytokine levels were analysed using the 10-spot V-plex kit Proinflammatory Panel 1 Mouse (Meso Scale Discovery ...
-
bioRxiv - Cell Biology 2021Quote: ELISA was performed to detect serum GH (rat/mouse growth hormone ELISA kit, Merck KGaA, Dermstadt, Germany), IGF-1 (mouse/rat IGF-1 ELISA kit ...
-
bioRxiv - Cell Biology 2020Quote: ... c-Src was detected using anti-Src (GD11 clone) antibody (1:200, Merck Millipore, Mouse, 05-184). Phosphorylated c-Src was assessed using anti-phospho-Src Tyr 418 antibody (1:100 ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse anti-Ascl2 (8F1, MAB4417) and rabbit anti-Sox9 (AB5535) and p57 (06556) were purchased from Merck Millipore ...
-
bioRxiv - Genomics 2022Quote: ... firstly with anti-DIG2 (sheep anti-mouse Ig digoxigenin conjugate, 1:200, Merck Millipore, Billerica, MA, USA) and antistreptavidin (1:25 ...
-
bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used for Western blotting were Goat-Anti-mouse IgG HRP conjugated (catalog #12-349; Merck) and Goat-Anti-rabbit IgG HRP conjugated (catalog #12-348 ...
-
bioRxiv - Cell Biology 2022Quote: ... 1:600-diluted rabbit anti-CENP-C (#554),55 mouse anti-HP1α (1:1,000, #MAB3584, Merck Millipore), rabbit anti-HP1β (1:800 ...
-
bioRxiv - Molecular Biology 2024Quote: PLA was performed by using Duolink™ In Situ Red Starter Kit Mouse/Rabbit (Merck, DUO92101-1KT) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for immunofluorescence in Drosophila: mouse anti-puromycin (clone 12D10, Merck Millipore) at 1:200 ...
-
bioRxiv - Immunology 2024Quote: ... and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP) from Merck Millipore was used at a dilution of 1:3500 ...
-
bioRxiv - Neuroscience 2023Quote: ... NBP1-92693AF647) and the oligodendrocyte transcription factor OLIG2 (Anti-OLIG2 clone 211F1.1 mouse mAb, Merck Millipore, MABN50A4). Samples were kept on ice throughout the isolation and staining procedure ...
-
bioRxiv - Biochemistry 2023Quote: ... Antibodies usedwere: mouse monoclonal (BIII-136) anti human Band 3 (B9277, Merck Life Science S.r.l., Milan, Italy). Mouse monoclonal (H68.4 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with primary antibodies against puromycin (mouse monoclonal 1:500, Merck), Par3 (rabbit polyclonal 1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with an anti-puromycin antibody (mouse monoclonal 1:500, Merck) combined with an anti-β-actin antibody (rabbit polyclonal 1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... and VEGF content was quantified using a custom-made Milliplex Mouse Cytokine/Chemokine Panel (Merck Millipore, USA), a magnetic bead-based multiplex immunoassay following the manufacturer instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... or with mouse monoclonal anti-α-tubulin antibody (1:1000; T6199; Sigma Aldrich; Merck KGaA, Darmstadt, Germany) in blocking solution ...
-
bioRxiv - Cell Biology 2024Quote: HepG2 cells or primary mouse hepatocytes were treated with either 50 μg/ml of cycloheximide (Calbiochem, Merck Millipore ...
-
bioRxiv - Biophysics 2024Quote: ... secondary antibodies goat anti-rabbit IgG and goat anti-mouse IgG conjugated with Atto 594 (Merck; 77671) and Abberior® STAR RED (Merck ...
-
bioRxiv - Biochemistry 2024Quote: ... wells were incubated with the NCAM specific monoclonal antibody OB11 (Merck, Cat.# C9672, mouse IgG1; 1:1000), followed by horseradish peroxidase (HRP)-conjugated anti-mouse IgG1 (Jackson Cat.# 115-035-205 ...
-
bioRxiv - Immunology 2024Quote: ... we added 50µL Biotin anti-mouse IgG (4µg/mL) (Sigma-Aldrich B7022; Merck Life Science; Hoeilaart, Belgium) to each well and incubated for 40 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Conjugated HEL-OVA solution was further purified from unconjugated proteins using a 50 kDa Amicon filter tube (Merck).
-
bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
-
bioRxiv - Biophysics 2021Quote: ... The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck). The concentrated sample was loaded onto a 320 ml HiLoad Superdex 75 (GE Healthcare Life Science ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: Total protein was extracted from the cells 48 h post-transfection by complete lysis-M buffer (Merck, USA). Twenty-five mg of total protein were loaded per well in a 12% SDS PAGE ...
-
bioRxiv - Microbiology 2019Quote: Supernatants of ham samples were clarified to eliminate proteins using the Carrez clarification kit (Merck KGaA, Darmstadt, Germany). Proteins were precipitated via the successive addition of 600 µL of Carrez reagent I and 600 µL of Carrez reagent II to 5 mL of supernatant ...
-
bioRxiv - Biophysics 2021Quote: ... we obtained microtubule-associated protein (MAP)-free neurotubulin by cation-exchange chromatography (1.16882, EMD SO3, Merck, Darmstadt, Germany) in 50 mM PIPES ...
-
bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were then separated in 12% gel by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, ISEQ00010), which were then block with 5% milk in TBS-T buffer at room temperature for 1 hour ...
-
bioRxiv - Molecular Biology 2022Quote: ... and SmWRKY27-GST were purified using a NucBuster™ Protein Extraction Kit (Cat. #71183; Merck KGaA, Darmstadt, Germany) for use in further EMSA experiments ...
-
bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... were separated by SDS-PAGE and protein was transferred to MeOH-activated Immobilon®-P membrane (Merck Millipore) using Whatman paper and a transfer buffer of 48 mM Tris ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein wet transfer was performed overnight at 4°C using an Immobilon®-FL PVDF membrane (Merck™). Next day ...
-
bioRxiv - Developmental Biology 2021Quote: ... The eluted protein was concentrated using ultrafiltration units with 10 kDa regenerated cellulose membrane (Amicon Ultra, Merck Millipore) and subsequently loaded on an equilibrated (50 mM Tris pH 8.0 ...
-
bioRxiv - Neuroscience 2020Quote: ... a short protein sequence expressed in the injected virus (1:400 rabbit anti P2A; ABS31, Merck, Darmstadt, Germany). In the third step ...
-
bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
-
bioRxiv - Developmental Biology 2022Quote: ... samples (100 µl culture broth) were subjected to protein precipitation followed by phospholipid removal (HybridSPE, Merck/Sigma Aldrich). The internal standard E2-d5 (Chromsystems ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluted fractions containing purified protein samples were concentrated using a 10 kDa Amicon centrifugal filter (Merck Millipore) and the elution buffer suspending the purified protein was replaced with EDTA (10 mM)-PBS (1x ...
-
bioRxiv - Biochemistry 2020Quote: ... One and a half μl of protein solutions were mixed on the target plate with 0.5 μl of the 20% 2,5-dihydroxybenzoic acid (Merck) solution in 20% ACN ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The protein samples were separated by 8% and 12% SDS PAGE and were transferred to PVDF membranes (Merck Millipore ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein-RNA complexes were eluted in pull-down buffer supplemented with 20 mM maltose (Merck, cat. No. 63418). The eluates were loaded on a NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gel ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated in 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
-
bioRxiv - Plant Biology 2023Quote: ... cells were transfected with plasmids encoding the respective heterologous proteins using the Genejuice transfection reagent (Merck, Darmstadt, Germany). After 48 hours of incubation at 37°C at 5% CO2 ...
-
bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
-
bioRxiv - Biochemistry 2022Quote: ... and protein-containing fractions were pooled and concentrated using the 10 kDa molecular weight cut-off concentrator (Merck). For monomeric C9 ...
-
bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1X PPI] and precleared by 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h ...
-
bioRxiv - Biochemistry 2023Quote: ... Rgl2RA-Halo or GST- Rgl2RA/GST-BRAFRBD fusion proteins were concentrated using a concentrator (10 KDa MWCO, Merck, Amicon Ultra centrifugal filters ...