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Citations for Merck :
1101 - 1150 of 1792 citations for GPN loop GTPase 3 GPN3 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenized tissue was centrifuged at 16,000 g for 45 min at 4 °C and the remaining supernatant was spun in 3 kDa cut-off Amicon® centrifugal filter tubes (Merck Millipore) at 14,000 g for 90 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM 2-mercaptoethanol] and then vortexed with an approximately equal volume of glass beads (212–300 mm) for 3 minutes (Merck KGaA, Germany). The homogenate was centrifuged at 12,000 g for 8 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... MCAD activity was measured using 3-phenylpropionyl-CoA (custom synthesis; 0.3 mM) as substrate and ferrocenium hexafluorophosphate as electron acceptor (Merck, 388297; 1.5 mM), followed by detection of 3-phenylpropenonyl-CoA using UHPLC (Wanders ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were removed from the samples by ultrafiltration at 3000 g and 30 min using Amicon Ultra filters (3 kDa cut-off, Merck, Darmstadt, Germany). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL of culture was sonicated for 3 s and 200 μL were loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Merck Millipore) connected to the CellASIC® microfluidic pump (Merck Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to buffer B without imidazole using 3 kDa Amicon® centrifugal filters (0.5 ml size) (both from Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cell Biology 2022Quote: ... shMYO10 #3 and shMYO10 #4 cell lines were generated using lentiviruses particles containing a non-target control shRNA (Merck, Cat Number: SHC016V-1EA) or shRNA targeting human MYO10 respectively (shMYO10 #3 ...
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bioRxiv - Biophysics 2020Quote: ... at different concentrations (20 mM, 200 mM and 500 mM) and concentrated using centrifugal ultrafiltration devices (3 kDa cut-off) (Merck India Pvt. Ltd.). This was followed by Gel filtration chromatography using Superdex 200 Increase 10/300 GL column (GE Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck, Buchs Switzerland). Memofl/fl littermates without Cre but treated with tamoxifen served as controls ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Cell Biology 2023Quote: ... The fixing solution was aspirated and wells washed 3 times in a washing buffer composed of DPBS containing 0.5% of BSA (Merck Life Science, Gillingham, UK). The washing buffer was left in the wells for 10 minutes following the last wash ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Microbiology 2024Quote: ... Samples were centrifuged (12,000 g for 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The purity was controlled by electrophoresing 3 µg of protein with SDS-sample buffer on a polyacrylamide gel (mPAGE™ 4-20% Bis-Tris Precast Gel, Merck, Darmstadt, Germany), followed by Bio-Safe Coomassie staining (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... to calculate the volume equivalent of 100 µg of protein before loading onto a 3 kDa molecular weight cutoff (MWCO) filter (Merck Millipore, Bayswater, Australia). Tryptic peptides were prepared using the FASP protocol as previously described (75) ...
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bioRxiv - Neuroscience 2021Quote: ... with a monoclonal anti-NeuN antibody (1:500; Mouse, MAB377, Merck Millipore) for brain sections in 0.5x blocking buffer supplemented with 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... In the case of O4 antibody (Merck Millipore, Germany; Cat. No. MAB1326), dilution was performed at 1:200 ...
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bioRxiv - Immunology 2021Quote: ... and then fluorescently conjugated secondary antibodies (Donkey anti-sheep IgG Cy5, Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies and dilutions were used: TPRX1 1:200 (Merck, HPA044922), SOX2 1:300 (R&D systems ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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bioRxiv - Genetics 2021Quote: ... using 1:500 dilution of Anti-CTCF antibody (07-729, Merck-Millipore) and 1:500 dilution of Anti-Lamin B1 antibody (ab65986 ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against Collagen IV (COL4, AB769, Merck Millipore, Billerica, MA, USA) and AIF1 (also known as IBA1 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation in secondary antibody and mounting in Mowiol (Merck, 475904). Confocal images were captured on a Zeiss Axiovert 200 upright microscope stand with LSM710 meta confocal scanner (63X objective NA 1.40) ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation in secondary antibody and mounting in Mowiol (Merck, 475904). Confocal images were captured on a Zeiss Axiovert 200 inverted microscope stand with LSM 710 confocal scanner (63X LD C-Apochromat objective NA 1.15) ...
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bioRxiv - Biochemistry 2021Quote: ... O-Linked N- Acetylglucosamine Antibody (ms, clone RL2, MABS157, Merck, 1:1000), AMDHD2 (ms ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg of CREB antibody (catalog #06-863, lot 2446851, Merck Millipore) or TCF4 antibody (CeMines ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were incubated with primary antibodies against NeuN (Merck ABN91 chicken) and calretinin (CR) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-CaM Kinase II α subunit antibody (05-532, Merck), mouse monoclonal anti-GAD67 antibody (MAB5406 ...
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bioRxiv - Plant Biology 2020Quote: ... were used when monoclonal Anti-HA antibodies produced in mouse (Merck; H9658) were chosen to detect HA recombinant proteins ...
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bioRxiv - Microbiology 2020Quote: ... a commercial mouse anti-flavivirus group antigen monoclonal antibody (MAB10216; Merck Millipore) diluted 1:1,000 in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was developed with a peroxidase-conjugated secondary antibody from Merck Millipore ...
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bioRxiv - Immunology 2022Quote: ... membranes were re-probed with anti-β-actin HRP-conjugated antibody (Merck), washed and developed using Pierce™ ECL Western Blotting Chemiluminescent substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: mouse monoclonals against vinculin (Merck Life Science) and CHOP (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with primary antibodies against Col1 (1:250, Merck #AB765P) or Gadd 153/CHOP (1:40 ...
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Developmental effects of oxytocin neurons on social affiliation and processing of social informationbioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were either mouse anti-TH (MAB 318, Merck Millipore), rabbit anti-EGFP (A11122 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were: rabbit polyclonal to Olig-2 (Merck Millipore, #AB9610) (dilution 1:500) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were: mouse anti-HuNu (1:200, Merck Millipore, #MAB1281), rabbit anti-TH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... using the following primary antibodies: mouse-NeuN (1:200, Merck-Millipore, MAB377), chicken-GFP (1:500 ...