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Citations for Merck :
1051 - 1100 of 1792 citations for GPN loop GTPase 3 GPN3 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Anti-Tie2 antibody ab33 (R&D Systems, Merck, cat. #05-584) for Tie2 IP or Tie2-Fc fusion protein (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... guinea pig polyclonal anti-doublecortin antibody (Merck, AB2253, 1:200 dilution), chicken polyclonal anti- NFH antibody (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... guinea pig polyclonal anti-DCX antibody (Merck, AB2253, 1:200 dilution), rabbit polyclonal anti-INA antibody (Merck ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was conducted with 1pg of a-FLAG antibody (F3165; Merck).
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bioRxiv - Microbiology 2023Quote: ... We employed α-VSV-G antibody coupled to sepharose beads (Merck) for ChIP-seq of NusG-VSVG ...
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bioRxiv - Microbiology 2023Quote: ... A commercial mouse anti-DENV complex monoclonal antibody (MAB8705; Merck Millipore) diluted 1:200 in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by secondary antibody anti-rabbit-HRP (1:4000, Merck Millipore). ECL Plus Western blotting reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained from Santa Cruz Biotechnology; antibody against p16INK4A (1:100; ZRB1437) was purchased from Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit IgG–FITC antibody sheep polyclonal (Merck, #F7512, 1:750), anti-mouse IgG–FITC antibody goat polyclonal (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse IgG–FITC antibody goat polyclonal (Merck, #F5387, 1:750), anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa-Fluor-labeled secondary antibodies and DAPI were obtained from Merck Millipore.
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bioRxiv - Molecular Biology 2024Quote: ... Primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:500) ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-AGE/methylgyoxal antibodies (clones 3D11 and 9F11, respectively, Merck) used at 1/200 ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-PSD95 antibodies (1:100, Cat. #MAB1596, Merck-Millipore). For GABAergic functional synapses ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hours and immunolabelled with anti-collagen VI antibody (Merck Millipore ...
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bioRxiv - Immunology 2024Quote: ... anti-CD3 and anti-CD28 antibody coated bead (Merck, Darmstadt, Germany) based stimulation for 24 or 48h ...
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bioRxiv - Molecular Biology 2024Quote: ... pre-conjugated with an anti-FLAG antibody (M2; Merck Millipore/Sigma) at 4°C for 1.5 h ...
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bioRxiv - Physiology 2024Quote: ... Immunoblots were next processed with anti-rabbit secondary antibody (Merck, AP132P) and visualized using the Western HRP substrate ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stained intracellularly for puromycin with AF488-puromycin antibody (Merck) using the FOXP3 fix/perm kit (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... The primary antibodies used were mouse α-GAPDH (Cat#: MAB374, Merck) in a 1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... or anti-V5 antibody (Merck, V8137; used at 1:1,000 dilution) in 5% milk in PBS-T overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: The vessel lumen was washed 3 times with PBS ++ (PBS with 1mM CaCl2, 0.5mM MgCl2) and fixed with 4% paraformaldehyde (PFA, Merck, #30525-89-4) at 37°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... and 10% ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Biochemistry 2022Quote: ... [2H7]22R-Hydroxycholest-4-en-3-one ([2H7]22R- HCO) was prepared from [2H7]22R-HC by treatment with cholesterol oxidase enzyme (Streptomyces sp., Merck, Dorset, UK) (11).
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bioRxiv - Microbiology 2021Quote: ... and 10 % Ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Physiology 2022Quote: ... and separation was carried out at 40°C using a ZIC-HILIC column (2.1 mm × 100 mm, 3 μm; Merck Sequant, Umea, Sweden). Isocratic elution was performed ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Slides were mounted in CitiFluor™ CFM3 mounting medium (proSciTech, Australia) containing 3 μg/uL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck, Germany) to stain nucleic acids ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in vehicle (Ringer solution; NaCl 140 mM, CaCl21.2 mM, KCl 3 mM and MgCl2 1 mM (Merck KGaA Darmstadt, Germany)) to a dilution of 5.5μg/μl ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...