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Citations for Merck :
1051 - 1100 of 1190 citations for Recombinant Yellow Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Developmental Biology 2024Quote: ... The lysates containing 300-500 µg of protein were supplemented with 20 mM DTT and 250 units Benzonase (250 U/µl, purity grade I, Merck) and incubated at 37°C for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... Purification of the labelled proteins was done by repeated filtration in a 3 kDa filter (Amicon Ultra-15 Centrifugal Filter Unit, Merck).
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation (20.000xg, 15 min, 4°C) and protein concentration was determined by Direct Detect® spectrometer (Merck). The proteasome activity was measured using a 96-well plate ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylated SR proteins were detected using a mouse monoclonal α-pan-phosphoepitope SR-specific antibody (1H4, Merck, Catalogue-No.: MABE50) at 0.025 µg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
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bioRxiv - Cell Biology 2020Quote: ... Stag-Tulp3 was then pulled down from the TEV digestion eluates by overnight 4°C rotational incubation using S-protein agarose beads (Merck, #69704). Beads were then eluted with Laemmli buffer and processed for SDS-PAGE in Novex Value 4-20% Tris-Glycine gels (Thermofisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Cancer Biology 2020Quote: CAF CMed was also separated into the metabolite (less than 3kDa) and protein (more than 3kDa) fractions using 3kDa centrifugal filters (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by incubation with primary antibodies (SOX2, Bio-techne, AF2018, 1: 1000 and α-Tubulin, Merck, T6199, 1:2000) and secondary antibodies (Donkey anti-Goat IRDye® 800CW ...
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bioRxiv - Bioengineering 2021Quote: ... and GEES protein fractions (2-16 µg) were processed by the MED-FASP protocol using Microcon 30k centrifugal ultrafiltration units (Merck, Darmstadt) [11] ...
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bioRxiv - Molecular Biology 2020Quote: ... The purity of the proteins were asses by SDS-PAGE and the fractions were pooled pure and concentrated using an Amicon Ultra concentrator (Merck Millipore) with a 10 KDa cut-off ...
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... To change the buffer in the M- and Z-AAT protein pools to Hank’s balanced salt solution (HBSS, Merck, Darmstadt, Germany) we used Vivaspin centrifugal concentrators with 10,000 MWCO (Vivaproducts ...
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bioRxiv - Systems Biology 2022Quote: ... The proteins were washed twice with acetone and subsequently solubilized using 100 mM ammonium bicarbonate (ABC) (Merck Sigma, Cat. No. 09830). Alternatively ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Microbiology 2020Quote: ... The collected supernatant was kept on ice until measurements of protein concentrations using Direct Detect® Spectrometer (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and concentrated using Amicon concentrator devices (molecular weight cut-off, 10 000 and for full-length protein 50 000; Merck Millipore). Recombinant His-tagged proteins (MA4376 ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore) and afterwards labelled with 100 µM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... and separating gel were used for SDS-PAGE and as a molecular weight standard protein marker (97, 66, 43, 29, 20, 14kDa) (Merck, India) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... the remainder was pre-cleared by co-incubation with 40 µL of PureProteome Protein A/G magnetic beads (Merck Millipore, LSKMAGAG10) per sample and rotation for 1 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Proteins (30 μg) were separated on an 8% SDS-PAGE gel and blotted onto a PVDF membrane (GE10600029 Merck, Darmstadt, Germany). Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... Standard immunostaining protocol was followed to stain for sarcomeric protein alpha-actinin and the coverslips were mounted on glass slides using fluoroshield (#F6182, Merck, Germany). Images were captured using the 60X oil immersion objective of the Olympus FV1000 confocal microscope (Olympus LifeScience ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE and were blotted onto an Amersham™ Protran® nitrocellulose membrane (Merck KGaA; Darmstadt, Germany) for 1.5 h using const ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... or human post-mortem total protein/ synaptoneurosome samples were then mixed into equal volumes of 2X Laemmli buffer (Merck: S3401- 10VL) and boiled for 10 minutes at 98 °C ...
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bioRxiv - Physiology 2023Quote: ... For protein extraction the tissue was homogenised in RIPA buffer with a cocktail of phosphatase inhibitors (Sigma-Aldrich, Merck, Gillingham, Dorset), using a sonicator three times for 30s each time ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...