Labshake search
Citations for Merck :
751 - 800 of 1190 citations for Recombinant Yellow Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... Eluted proteins were concentrated in a 10 kDa molecular weight cutoff (MWCO) centrifugal filter (Amicon, Merck).
-
bioRxiv - Plant Biology 2023Quote: ... and proteins were blotted onto a 0.45 µm PDVF Immobilon-P membrane (Merck Millipore, ref. IPVH00010) after electrophoresis ...
-
bioRxiv - Bioengineering 2023Quote: ... the chips were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Merck, Schiphol-Rijk, The Netherlands) in sterile PBS (100 μg/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (Amersham Hybond ECL; GE Healthcare, RPN303D, Merck, Darmstadt, Germany). After blocking in 5% (w/v ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the proteins were concentrated to 15 – 30 mg/ml concentration using Amicon Ultra Centrifugal filters (Merck) via short (2 – 5 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Merck Millipore, cat #IPFL00010). After blocking with Intercept TBS Blocking Buffer (LI-COR ...
-
bioRxiv - Neuroscience 2024Quote: ... Equal protein amounts were separated by SDS PAGE and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
-
bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 250 μg of proteins were emulsified with an equivalent volume of Freund’s Incomplete adjuvant (Merck F5506) and injected post 21 days ...
-
bioRxiv - Biophysics 2024Quote: ... The eluted protein was concentrated with a 15 ml 10000 MWCO spin filter (Merck Millipore Ltd.). The sample was centrifuged at 20.000 g for 15 min to remove precipitated protein prior to being loaded onto a HiLoad 16/600 Superdex 200 size exclusion chromatography (SEC ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
-
bioRxiv - Biochemistry 2024Quote: Optional concentration of protein samples using Amicon® Ultra centrifugal filters (Merck Millipore Ltd., Carrigtwohill, IRL) was performed prior to buffer exchange to reduce sample volume ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant (i.e., crude protein) was filtered through a 0.45 μm pore size membrane filter (Merck), applied onto HisTrap FF crude column (Cytiva ...
-
bioRxiv - Biochemistry 2024Quote: ... and the proteins were subsequently transferred to a PVDF membrane (#IPVH00010, Merck Millipore, Burlington, MA, USA). The membranes were washed with TBS-T (20 mM Tris (pH 7.5) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cell lysate was prepared and protein concentration was measured by Direct Detect® spectrometer (Merck). An equal amount of proteins (50 µg ...
-
bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein mixtures were incubated with 40μl PBST washed Streptavidin agarose resin (50% slurry, 69203-3 Merck) and incubated for 2h at room temperature on a roller ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins contained in BALF and TS were concentrated 5x using an Amicon Ultra column 100kDa (Merck Millipore) and 20 min centrifugation at 4000xg and samples were stored at −80°C ...
-
bioRxiv - Biophysics 2020Quote: ... the protein was concentrated by centrifugation using Amicon Ultra 15-30k centrifugal filters (Merck, Kenilworth, NJ, USA) at 3452 x g ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were run in SDS-PAGE and transferred to Immobilon Polyscreen PVDF transfer membranes (#IPVH00010, Merck Millipore) or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: ... Target protein expression was detected using Immobilon™ Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) according to manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
-
bioRxiv - Microbiology 2019Quote: ... Total protein (30 μg) was separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Merck, Germany) using a transfer apparatus (BioRad ...
-
bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
-
bioRxiv - Bioengineering 2021Quote: ... the purified protein containing fraction was concentrated (Centriprep® Centrifugal Filters Ultacel® YM - 50, Merck Millipore).
-
bioRxiv - Pathology 2021Quote: ... The proteins were cleaned using Amicon Ultra-4 Centrifugal Filter Devices (15 ml, 10 kD; Merck Millipore) to remove the elution buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Two microlitres of the supernatant was used for protein quantification using the Direct Detect system (Merck Millipore). The acquired concentrations of intracellular c-di-GMP were normalized to the total protein content.
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were separated by SDS–PAGE (4-20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
-
bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were transferred on an Immobilon™-P Membran (PVDF, 0.45 µm, Art. No. IPVH00010, Merck Millipore) for 16 h at 15 V using a Mini Trans-Blot® Cell (Art ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto a PVDF membrane (pore size 0,2 μm, Merck, cat ...
-
bioRxiv - Biochemistry 2022Quote: ... The refolded protein was concentrated with Amicon Ultra-15 Centrifugal Filter Units (100 kDa NMWL, Merck Millipore) and remaining urea removed with a PD10 desalting column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: Denatured proteins were separated by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore IPFL00010). Membranes were incubated in a casein-based blocking buffer (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
-
bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, Burlington, MA) by electroblotting ...
-
bioRxiv - Microbiology 2020Quote: ... Lentivirus stocks were concentrated 25-fold using centrifugal protein concentrators (Amicon Ultra, 10-kDa cutoff; Merck, Poland) and stored at - 80°C.
-
bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... proteins were separated from each sample by centrifugation through Amicon ® Ultra Centrifugal Filters (Merck, Millipore Ltd.) at 21.000 x g for 10 min at 4°C ...
-
bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore ...
-
bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica ...
-
bioRxiv - Developmental Biology 2019Quote: ... were transformed with the pET-UBE2D3 plasmids and protein expression was induced with 0.4 mM IPTG (Merck) for 3h at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Secreted proteins were collected after a 72-h incubation by Amicon Ultra-15 Centrifugal Filter Units (Merck) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... The eluted protein was concentrated using an Amicon-Ultra-15 centrifugal filter (MWCO 30kDa) (Merck Millipore, USA), spin down 15 min in a bench-top centrifuge (4°C ...
-
bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (PVDF, Merck Millipore) for immunoblot analysis ...
-
bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
-
bioRxiv - Cell Biology 2020Quote: ... After eluting and concentrating the cleaved protein in Amicon Ultra-15 Centrifugal Filters 50K MWCO (Merck Millipore), size exclusion chromatography (SEC ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins in each lysate were resolved by SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck). The blots were incubated with the following primary antibodies ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by SDS-PAGE system and transferred to Immobilon-P membrane (Merck KGaA, Darmstadt, Germany). Immunoreactions were detected according to standard methods described previously [Yoshitake et al ...