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Citations for Merck :
901 - 950 of 1010 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... To change the buffer in the M- and Z-AAT protein pools to Hank’s balanced salt solution (HBSS, Merck, Darmstadt, Germany) we used Vivaspin centrifugal concentrators with 10,000 MWCO (Vivaproducts ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: The wing-type electrodes are electrically characterized by measuring their impedance spectra at frequencies between 102 and 106 Hz in Ringer’s solution (Merck, Darmstadt, Germany). A three-electrode setup is used which is comprised of a Pt counter electrode ...
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bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... fixed with a 4% PFA solution and stained for tyrosine hydroxylase (TH; rabbit anti-TH, 1:1000, Cat#: 657012, Merck Millipore) and neurobiotin (Streptavidin Alexa Fluor conjugate 647 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 2 μl of the enzyme β-glucuronidase (from Helix from Pomatia enzyme aqueous solution, ≥ 100.000 units/mL; Merck, Darmstadt, Germany) to deconjugate DEHP metabolites and BPA ...
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bioRxiv - Microbiology 2020Quote: ... and the other stored at −80 °C in a sterile 2 ml cryovial containing 1 ml of a 30 % glycerol solution, prepared by diluting glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Bioengineering 2022Quote: ... was used to mimic tissue-like scattering conditions and Nigrosin (0.62 v/v% of Nigrosin stock solution [0.5 mg/mL Nigrosin in deionised water, Merck, 8005-03-6]) was added to mimic tissue-like absorption.
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bioRxiv - Microbiology 2022Quote: ... samples were serially diluted in peptone physiological salt solution (PPS; Tritium Microbiology, The Netherlands) and appropriate dilutions were spread plated on TOS-Propionate agar (Merck, Germany). Plates were incubated anaerobically at 37°C for 48-72 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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bioRxiv - Systems Biology 2024Quote: ... the acidified peptide solution was cleaned-up on self-packed StageTips (70) accommodating four discs of Empore C18 material (Merck Supelco). The material was prepared by consecutive application of pure ACN (Biosolve Chimie) ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... Supplements were prepared as 50 mM stock solutions in Milli-Q water and sterilized by filtration with syringe filters (Millex-GV, 0.22 µm, PVDF, Merck, Darmstadt, Germany). Each inoculated main culture was divided into three or four different wells (biological replicates ...
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bioRxiv - Cancer Biology 2023Quote: ... Conjugated AB were eluted in 30 μL antibody stabilizer solution (Standard Biotools, CA, USA) supplemented with 0.05 %sodium azide (Merck, Darmstadt, Germany) to a total volume of 50 μL stored in low attachment 1 mL Eppendorf tubes.
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated in a 10% (v/v) HS PBS solution supplemented with primary mouse anti-β-tubulin III antibody (1:100; Merck) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were resuspended in 8 M urea solution in 100 mM Tris-HCl and deposited on top of Microcon®-30K devices (Merck). Samples were centrifuged at 9400 × g ...
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bioRxiv - Neuroscience 2023Quote: ... we applied with unlabeled (S)-PhPrM and (S)-PhPr solutions to the origin point on a reversed-phase plate coated with fluorescent indicator (RP-18 F254S, Merck Millipore). The developed spots were detected with ultraviolet light (254 nm).
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Neuroscience 2023Quote: ... we transferred experimental and control 76 flies to a vial containing food covered with 20µl of all transretinal (ATR) solution (100mM ATR in 100 %ethanol; Sigma Aldrich R2500, Merck, Germany) and wrapped in aluminium foil.
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bioRxiv - Cancer Biology 2023Quote: The Propidium Iodide (PI) working solution was prepared consisting of 4.35 mL 1 g/L sodium citrate dehydrate (Merck, cat# 6448), 0.5 mL 1 mg/mL DNAse-free RNAase A (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were washed 3x with PBS before incubation in a solution of anti-Rabbit-IgG-ATTO 594 (Merck, #77671-1ML-F) as secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... Triton X-100 and incubated for 48 h in the sealed jars in the presence of a perforated tube containing vermiculite saturated with 7.43 g/100 mL KMnO4 solution (Merck, Darmstadt, Germany) as described by Ophir et al ...
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bioRxiv - Microbiology 2023Quote: ... the stabilized pellets were enzymatically lysed for 30 min in a lysozyme solution (15 mg/ml in TE buffer; L4919 Sigma-Aldrich, Merck) combined with 1:10 (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... in T-BST for 1 h at room temperature or Re-Blot Plus Strong Antibody Stripping solution for 15 min at room temperature (Merck, #2504). Quantification of protein bands was performed using ImageJ analysis software (release 1.53c).
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bioRxiv - Bioengineering 2024Quote: ... The content of sodium was calculated using a calibration curve made by measuring dilutions of a standard sodium solution (Merck, 1.70238). To eliminate the interference of the potassium signal ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Biochemistry 2024Quote: ... pyrene–labeled phalloidin–stabilized WT or mutant F–actin and the myosin solutions were treated with apyrase (Merck KGaA, Darmstadt, Germany) to remove residual nucleotide ...
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bioRxiv - Immunology 2024Quote: ... ankle tissues were soaked in 4% (v/v) paraformaldehyde phosphate buffer solution (Fujifilm Wako) overnight and then replaced with OSTEOSOFT (Merck Millipore) every three days over 11 days for decalcification ...
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bioRxiv - Biochemistry 2024Quote: ... 18 µL of a 20 µM solution of LmPDTN56C was mixed with 2 µL 100x SYPRO Orange (Merck kGaA, Darmstadt, Germany). The samples were then subjected to a temperature ramp spanning from 35 to 95 °C at 1 °C/min recording the fluorescent emission (Exc ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 million cells per mL were suspended in 1 mL of a 1.2% sodium alginate solution (Merck, Saint-Quentin-Fallavier, France). Beads were formed by dripping the cell suspension into a sterile 100 mM CaCl₂ solution (VWR ...
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bioRxiv - Developmental Biology 2024Quote: ... The fins were then washed again with DPBS and then digested for 20 min in Collagenase P solution (1mg/ml, Merck Millipore) in Leibovitz’s L-15 medium ...
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bioRxiv - Bioengineering 2021Quote: Culture of human BM-MSCs and NE-MSCs with biomaterials performed in Corning® Costar® ultra-low attachment 24 well plates (Merck KGaA, Darmstadt, Germany) with Gibco™ α-MEM (Thermo Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... The sections were subsequently dehydrated through a series of ethyl alcohol solutions and then placed in xylene before being coverslipped with Entellan mounting media (Merck Millipore, USA).
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were thawn on ice and resuspended in lysis buffer (5 mL/g pellet: 50 mM Na2HPO4/KH2PO4, 150 mM NaCl, 0.2 % Tergitol™ solution (Merck, Darmstadt, Germany), and 2 mM DTT ...
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bioRxiv - Bioengineering 2021Quote: ... and the remaining skin was cut in strips of approximately 4 cm length and 1 mm width and finally incubated in 10 ml skin digestion solution (2.0 U/mL dispase D4693, Merck KGaA, in PBS-) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... degradation of any residual cDNA in the viral solution from production was first assured by benzonase nuclease treatment (70746, Merck Millipore, Germany). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... fillet dissections of L3 larvae or pharate adults were performed in modified HL3 solution ([HL3-EGTA] 70 mM NaCl, 5 mM KCl, 10 mM NaHCO3, 20 mM MgCl2, 5 mM trehalose (Merck KGaA, 108216), 115 mM sucrose (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 10 min incubation at RT with 50% (v/v) lead citrate solution in decocted H2O containing 80 mM lead citrate (Merck, Darmstadt, Germany) and 0.12 M trisodium citrate (AppliChem ...
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bioRxiv - Developmental Biology 2022Quote: ... for 20 minutes and eosin Y (Wako, 058-00062) for 15 seconds before dehydration and mounting in Entellan solution (Merck Millipore, HX73846161). Images were captured using a Zeiss Axio Imager Z2 microscope and Zen 2.3 Pro software.
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bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...