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Citations for Merck :
801 - 850 of 1010 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Infected RBCs were lysed with saponin (0.15%) in PBS and washed 3 times with protease inhibitor cocktail solution (PI, cOmplete Mini; Merck). Proteins were extracted using 1% Triton-X 100 (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... the buffer was replaced with complex IV staining solution [1 mg/mL DAB (3,3′-Diaminobenzidine tetrahydrochloride hydrate; Merck D5637), 1 mg/mL cytochrome c (Merck C7752)] in complex IV buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... 2 washes with 1X HBSS and an incubation in a trypsin-based digestion solution containing DNAse I (Merck, D5025) for 15 minutes at room temperature (RT) ...
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bioRxiv - Bioengineering 2024Quote: ... this TEOS/ethanol mixture was introduced into a solution containing 100 mL ethanol and 20 mL NH4OH (30%, Merck), and stirred for 2 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The methanol-fixed samples were transferred to 3% glutaraldehyde solution (Agar scientific) in 0.1 M sodium phosphate buffer (Merck). Dehydration was performed with ascending ethanol series (30% ...
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bioRxiv - Microbiology 2024Quote: ... These mycelia were again filtered through sterilized Miracloth into 25 ml of enzyme solution for cell wall digestion (0.75% lysing enzyme [Merck] and 0.6% cellulase [Yakult ...
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bioRxiv - Microbiology 2024Quote: ... 6.25 x 106 IFU of Chlamydia was mixed with 7.5 µg of shuttle vector in 100 µl of calcium chloride (CaCl2) solution containing 100 mM CaCl2 (Merck) and 20 mM Tris (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Micrococcin P1 was produced as described previously and solubilized to a concentration of 10 mg/ml in dimethyl sulfoxide (Sigma-Aldrich).30 Nisin A was obtained as part of a commercial fermentate which was solubilized to a concentration of 1 mg/ml in a 0.05% acetic acid solution (Merck). AuresinePlus was solubilized to a stock concentration 1 mg/ml in a buffer containing 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cancer Biology 2024Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
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bioRxiv - Microbiology 2024Quote: ... the beads in each well were covered with 500 μl of NaCl 0.9% saline solution and another 500 μl of a 0.1% crystal violet (Merck, Germany) in saline for 1 min ...
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bioRxiv - Biochemistry 2024Quote: The monomeric recombinant peptide was prepared by resuspending it in a solution of 1% NH4OH (Merck Cat. No. 221228) in PBS buffer at a concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... paraformaldehyde solution (ProSciTech, C004) and blocked with Organoid Wash Buffer (DPBS (GibcoTM, 14190144) + 0.1% (w/v) TritonX-100 (Merck) + 0.2% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... then scraped and lysed in RIPA lysis buffer solution supplemented with 1X protease and phosphatase inhibitor cocktail (Merck, PPC1010). Protein concentrations were quantified using Bradford reagent ...
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bioRxiv - Neuroscience 2021Quote: ... spheroids from each microwell were collected by firmly pipetting medium in the well up and down and transferred into Corning® non-treated culture dishes (Merck, CLS430591-500EA) in TeSR™-E6 (StemCell Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... EB from each microwell were collected by pipetting up and down the medium several times and transferred into Corning® non-treated culture dishes (Merck, CLS430591-500EA) in EB medium containing DMEM/F12 GlutaMAX (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... were supplemented with or without S100A8 or S100A8/9 recombinant protein and plated to 24 corning corstar ultra-low attachment multiple well plates (Merck, Cat. #CLS3473, Germany). Tumorsphere were imaged using IncuCyte S3 Live-Cell Imaging Analysis System (Ann Arbor ...
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bioRxiv - Biophysics 2021Quote: ... The solution was concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, MA, USA) and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Counterstaining of nucleus was performed using Mayer’s hemalum solution and slides were mounted with Kaiser’s glycerol gelatin (the Kit and all reagents were obtained from Merck Millipore). Images were acquired as described above.
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bioRxiv - Developmental Biology 2021Quote: ... for 5 min at room temperature to loosen the zona pellucida and then treated with a hypotonic solution (1:1 mixture of 1.2% sodium citrate and 60% fetal bovine serum, FBS; Merck KGaA) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were finely dissected into tiny pieces and enzymatically digested into single cells by incubating them in a dissociation solution containing 0.125 mg/ml Collagenase (Merck, C9891), 1 U/ml Dispase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... they were washed again in PBS (three times, each for 5 min) and mounted with antifade solution (Cat.no. S7114, Sigmsa-Aldrich, Merck, USA). Images were acquired using a Leica DM 2500 fluorescent microscope (Leica ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were incubated with a solution containing 1/50 phalloidin alexa fluor 488 in PBST supplemented with 5% DMSO (Merck) and covered with parafilm (Bemis ...
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bioRxiv - Immunology 2021Quote: ... which contained as little saliva as possible were placed in a weighed Eppendorf tube and processed with 4x weight/volume of sputolysin working solution (Merck). Afterwards ...
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bioRxiv - Pathology 2020Quote: ... was performed placing the sections in a 800 ml glass container filled with the retrieval solutions (10 mM EDTA in Tris-buffer pH 8, Merck), irradiate in a household microwave oven at full speed for 8 min ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGLUT1, 1:1000, MAB5502, Merck) or rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Bioengineering 2020Quote: ... The solution was removed from the centrifuge and filtered through a syringe filter with a 25 mm diameter (Merck Millipore) into a clean syringe at least twice to ensure no precipitates were present ...
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bioRxiv - Neuroscience 2021Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... BM cells harvested from moribund mice were cytospun and stained with Giemsa’s azur-eosin-methylene blue solution (Merck, cat.no. 109204).
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bioRxiv - Bioengineering 2021Quote: ... 99%+, Figure S1(d)), and dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP, 42% solution in methanol) were obtained from Merck–Sigma Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... surface sterilized with 10% (v/v) bleach solution and plated on agar plates containing one-half strength Murashige and Skoog (MS) salts (Merck) pH 5.7 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGlut1, 1:1000, MAB5502, Merck) and rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... 20 μL of the MTT solution was added to each well (5 mg/ml, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Then ...
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bioRxiv - Cell Biology 2021Quote: ... The malachite green reagent was prepared by mixing solution A (17 mg of Malachite Green Carbinol base dye (229105, Merck) in 20 mL 1 N HCl ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM stock solution was prepared by dissolving ROCKi powder in 100% dimethyl sulfoxide (DMSO, Merck Life Science, UK, #D8418) and filtered through 0.22µM syringe filters ...
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bioRxiv - Microbiology 2021Quote: ... Japan) and the plaques were visualized by using a Giemsa’s azur-eosin-methylene blue solution (#109204, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Neuroscience 2022Quote: ... After centrifugation (500 x g, 5min) the tissue was mechanically disintegrated in a solution containing 170 ug/ml DNAse (#10104159001, Merck) in EBSS ...
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bioRxiv - Developmental Biology 2020Quote: ... pregnant mice were injected intraperitoneally with 375 μg doxycycline (75 μl of a filter sterilized, 5 mg/ml solution of Doxycycline Hyclate (Merck) dissolved in PBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines WM35 and WM793B were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution (Merck, Darmstadt, Germany). For generation of conditioned melanoma media for immune cell inhibition experiments tryptophan (Merck ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µg of protein solution was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 ...
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bioRxiv - Immunology 2021Quote: All samples for CD-MS measurements were first buffer exchanged into 500 mM ammonium acetate solution (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore), unless otherwise stated ...
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bioRxiv - Bioengineering 2022Quote: ... were rinsed with PBS and incubated in 500 μL of cell lysis solution containing 0.2% (v/v) Triton-x-100 (Merck, 1.08603.1000) in 5 mM MgCl2 (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the final ribosome solution was diluted with 70S buffer 10-fold and then concentrated using Amicon Ultra (30 kDa cut, Merck) three times.
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bioRxiv - Neuroscience 2022Quote: ... Cubes of 1 cm3 were cut and incubated overnight in an aqueous solution of 150 mM NaCl (S9888, CAS 7647-14-5, Merck) and 10 µM fluorescein (46955 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... were sonicated in 1 M KOH solution (6592-3705, Daejung) for 30 min and then washed with Milli-Q water (Direct 8, Merck) to remove remaining KOH solution ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1% BSA/PBS and subjected to the Click-iT reaction in a solution containing 10 mM sodium ascorbate (Merck), 0.1 mM azide-PEG3-biotin conjugate (Merck ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: SBECD solutions with increasing concentrations were prepared using ultrapure water (Millipore Direct-Q 5UV system, Merck Millipore, Burlington, MA, USA) and the same amounts of OTX008 were tested to dissolve in them to reveal the solubilization ability of SBECD on OTX008 ...
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bioRxiv - Plant Biology 2024Quote: ... The assay was carried out by replacing the distilled H2O in the wells by a 100 µL reaction solution of 100 µM Luminol or L-012 (Merck), 20 µg mL-1 horseradish peroxidase (Merck ...