Labshake search
Citations for Merck :
901 - 950 of 1092 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
-
bioRxiv - Pathology 2023Quote: ... 95% and 100% ethanol solutions (5 min each) and subsequently washed twice in xylol (5 min each) and embed using Eukitt (#03989, Merck).
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed using formaldehyde (1% V/V in H2O) for 10 min at RT before supplementation with 125 mM glycine solution (Merck) and agitation for 5 min ...
-
bioRxiv - Biochemistry 2023Quote: ... the reduced cysteine residues were blocked employing a 10 mM solution of S-methyl methanethiosulfonate (MMTS) (Merck KGaA, Darmstadt, Germany) at room temperature for 10 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... the solution was transferred to a Millipore Amicon Ultra 30 kDa cut off membrane centrifugal filter unit (Merck Millipore, USA) (prewashed twice to remove glycerol ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were caught and rinsed in 1x PBS (3 × 15 min) prior to incubation in blocking solution (5% NDS (Merck) in 0.3% PBS-Triton-X-100 ...
-
bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS ...
-
bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 500 μg of chimeric InvP protein in-solution and mixing with an equivalent volume of Freund’s complete adjuvant (Merck F5881). For the subsequent five booster doses ...
-
bioRxiv - Biophysics 2023Quote: ... for 15 min at 20°C and the stamps were dried under a nitrogen flow A solution was then prepared by mixing 40 µL human fibronectin (Merck) and 960 µL of demineralized water ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were lysed in a protein extraction solution (∼10 µL/ mg tissue) consisting of 0.32M sucrose with protease inhibitors (Merck #4693159001) and phosphatase inhibitors (Pierce # A32957 ...
-
bioRxiv - Neuroscience 2024Quote: ... the reaction was quenched with three subsequent washes using the quenching buffer described in Loh et al.24 based on Tyrode’s solution supplemented with 10 mM sodium azide (Merck, S2002), 10 mM sodium L-ascorbate (Merck ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87 ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Adult mosquitoes were provided ad libitum access to 10percent sugar solution (8percent sucrose +2percent glucose +0.05percent methyl paraben +5percent vitamin syrup (Polybion SF Complete, Merck Ltd.)) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 100 µL of the washed GUV solution was transferred to an 18-well imaging chamber and supplemented with 10 mM Magnesium-Ionophor I (Merck). Once the sample was placed on the confocal microscope with an incubation chamber held at 37 ◦C for 2 h ...
-
bioRxiv - Physiology 2024Quote: ... the pancreas was perfused via the pancreatic duct using an ice-cold collagenase solution (Collagenase P, 11213865001, Merck/Sigma Aldrich) with a concentration of 0.63 mg/ml ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were subsequently freeze-substituted in a solution containing 0.2 % (v/v) osmium tetroxide (ChemPur, Germany) and 0.1% uranyl acetate (w/v) (Merck KGaA, Germany) in acetone ...
-
bioRxiv - Systems Biology 2024Quote: ... with 100 µl of a 2 % w/v solution of Evans blue tracer in 0.9 % saline (Merck Ltd, Poole, UK) or i.v ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then washed with three rounds of PBS before incubation in 200 µl p-Nitrophenol phosphate substrate solution for ELISA (Merck) for 10 min ...
-
bioRxiv - Microbiology 2024Quote: ... Wells were again washed three times with PBS and bound alkaline phosphatase quantified using a p-Nitrophenol phosphate substrate solution for ELISA (Merck) and A405 readings.
-
bioRxiv - Neuroscience 2024Quote: ... The inside of the tip of the KCl-containing channel was silanized using Silanization Solution I (85126, Merck-Sigma Aldrich) and then filled with Potassium Ionophore I Cocktail A (99311 ...
-
bioRxiv - Plant Biology 2024Quote: ... a second extraction was performed by adding 30 mL of extraction solution consisting of 95% (w/v) methanol (Merck Uvasol), 4.95% (w/v ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... slides were incubated in 60% isopropanol for 20 sec followed by the incubation in Oil Red O solution (Merck 1.02419.0250) for 10 mins ...
-
bioRxiv - Systems Biology 2024Quote: ... rehydrated sections [20] in a 800 ml glass container filled with the retrieval solutions (EDTA pH 8; 1 mM EDTA in 10 mM Tris-buffer pH 8, Merck Life Science S.r.l.,Milano ...
-
bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with blocking solution for 1 h and then with mouse monoclonal anti-NeuN or SMI 32 antibody (1:200; Merck Millipore ...
-
bioRxiv - Neuroscience 2024Quote: ... blocked for 2 h in blocking solution (5% normal goat serum from Vector Laboratories, 0.5% Triton X-100 from Merck in PBS ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by signal development for 6–24 h in a solution containing nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (Merck). The samples were covered with glass coverslips using CC/Mount (Merck) ...
-
bioRxiv - Bioengineering 2024Quote: A 5 mL Terumo syringe containing a solution of 80 mg mL−1 uncrosslinked GelMA and 2.5 mg mL−1 LAP (Merck 900889) in PBS was loaded onto an AL1010 syringe pump (World Precision Instruments) ...
-
bioRxiv - Cell Biology 2024Quote: ... they were incubated for 1 hour at RT with a blocking solution that consisted of 5% chemiBLOCKER (Merck, Darmstadt, Germany) in 0.1% PBS Triton X-100 ...
-
bioRxiv - Cancer Biology 2020Quote: ... the region surrounding the gRNA target sites were amplified by PCR with KOD Hot Start Polymerase (Merck) according to manufacturer’s instructions with the following primer pairs ...
-
bioRxiv - Microbiology 2020Quote: ... the SAG1 promoter and the GFP-coding fragment was amplified by PCR using the KOD polymerase (Merck) and the ML2477/ML2664 primers that included regions for integration by double homologous recombination ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Gene insertion was verified by PCR using the primer pairs pETUpstream/DuetDOWN or DuetUP2/T7 Terminator (Merck) with Enc or mSOG-containing plasmids as template DNA ...
-
bioRxiv - Biophysics 2021Quote: ... Freshly excited slices were incubated for 30 min at 37°C in a solution of Nile Red (Cat. No. 72485-100MG, Sigma-Aldrich/Merck) pre-dissolved in methanol at a concentration of 1 mg/ml and diluted 1:20 in PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... The absorbance of the pink solution from each isolate was measured and recorded at 530 nm using spectrophotometer (Thermo Spectronic, Merck, SA).
-
bioRxiv - Immunology 2021Quote: ... Color was developed in the dark for 5-10 min before adding 50 µL of 2 M H2SO4 stop solution (Merck, Germany). Optical density was read at wavelength 450 nm using the microplate reader (SpectraMax ID3 ...
-
bioRxiv - Biochemistry 2020Quote: ... the solutions were filtered using 96-well plates with a 0.22 μm filter (MultiScreen® Filter Plates, Merck Millipore, MA, USA). Then ...
-
bioRxiv - Biochemistry 2021Quote: The initial buffer in the huPrP solution was exchanged to ultrapure water by triplicate diafiltration using an Amicon Ultra 0.5 ml centrifugal filter (Merck & Co., USA) with an NMWL cutoff of 3 kDa ...
-
bioRxiv - Microbiology 2021Quote: ... The diluted solution was concentrated to approximately 5 mL using 30 kDa molecular weight cutoff Amicon Ultra centrifugal filters (Merck KGaA), passed through a Millex-GP syringe filter with 0.22 μm pore size (Merck KGaA) ...
-
bioRxiv - Cell Biology 2020Quote: ... To change the buffer in the M- and Z-AAT protein pools to Hank’s balanced salt solution (HBSS, Merck, Darmstadt, Germany) we used Vivaspin centrifugal concentrators with 10,000 MWCO (Vivaproducts ...
-
bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: The wing-type electrodes are electrically characterized by measuring their impedance spectra at frequencies between 102 and 106 Hz in Ringer’s solution (Merck, Darmstadt, Germany). A three-electrode setup is used which is comprised of a Pt counter electrode ...
-
bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
-
bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... fixed with a 4% PFA solution and stained for tyrosine hydroxylase (TH; rabbit anti-TH, 1:1000, Cat#: 657012, Merck Millipore) and neurobiotin (Streptavidin Alexa Fluor conjugate 647 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 2 μl of the enzyme β-glucuronidase (from Helix from Pomatia enzyme aqueous solution, ≥ 100.000 units/mL; Merck, Darmstadt, Germany) to deconjugate DEHP metabolites and BPA ...
-
bioRxiv - Microbiology 2020Quote: ... and the other stored at −80 °C in a sterile 2 ml cryovial containing 1 ml of a 30 % glycerol solution, prepared by diluting glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
-
bioRxiv - Bioengineering 2022Quote: ... was used to mimic tissue-like scattering conditions and Nigrosin (0.62 v/v% of Nigrosin stock solution [0.5 mg/mL Nigrosin in deionised water, Merck, 8005-03-6]) was added to mimic tissue-like absorption.
-
bioRxiv - Microbiology 2022Quote: ... samples were serially diluted in peptone physiological salt solution (PPS; Tritium Microbiology, The Netherlands) and appropriate dilutions were spread plated on TOS-Propionate agar (Merck, Germany). Plates were incubated anaerobically at 37°C for 48-72 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...