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Citations for Merck :
1001 - 1050 of 1092 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... ileum and colon sections were deparaffinated in histolene and rehydrated in a descending series of ethanol (100%, 96%, 70%) followed by hematoxylin solution (H&E, Merck, Darmstadt, Germany) for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were directly immersed in CUBIC-L solution (10 wt % N-Butyldiethanolamine [Tokyo Chemical Industry, B0725], 10 wt % Triton X-100 [Merck, 1.08603.1000] and 80 wt % MilliQ water) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were left to soak for no less than 24 hours in either plain water (for the cryptic/plain treatment) or a 2.5% Bitrex™ aqueous solution (5 g denatonium benzoate (Merck, Darmstadt, Germany) dissolved into 200 ml water ...
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bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated 48 hours at 37°C with BrdU (15 μM, stock solution 30 mM in H2O) (Merck, Darmstadt, Germany). After washing two times with warm PBS the cells were pre-extracted with ice cold NuEx buffer and fixed with 2% (w/v ...
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bioRxiv - Zoology 2023Quote: ... about 10 mL of CLE flower extract was added to a 90 mL aqueous solution of 1 mM AgNO3 (Merck Laboratories, India) and kept at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Frozen larvae were thawed for two min in a solution of 50:50 5% bleach (Danklorix, CP GABA, Hamburg, Germany) and Milli-Q (Merck, Darmstadt, Germany) water for surface sterilization (Amiri et al. ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Fixation was performed by adding 100 μL of a cold 80% vol/vol solution of Acetone (Sigma-Merck, St. Louis, MO, USA) in DPBS and incubating the plates for 10 min at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The ability of venoms to clot fibrinogen was assessed by incubating 200 µL of a 6 mg/mL bovine fibrinogen solution (Merck, Darmstadt, Germany) at 37 °C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... we transferred experimental flies to a vial containing food covered with 40 µl of all-trans-retinal (ATR) solution (100 mM ATR in 100% ethanol; Sigma Aldrich R2500, Merck, Germany) and wrapped in aluminium foil to limit light exposure ...
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bioRxiv - Molecular Biology 2024Quote: ... The tissue was washed in PBS and incubated in 5 mM EDTA solution in PBS (pH 8; Merck Millipore, Burlington, MA, USA) at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... pH 7.5) and the capsid solutions were filtered once (centrifugal filter unit Ultrafree MC, pore size of 100 nm, Merck KGaA, Darmstadt, Germany). The concentrations of the P1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The stock solution was added at 1% v/v to Plasmax cell culture medium (CancerTools cat# 156371) supplemented with 5% FBS (Merck cat# F7524) and penicillin/streptomycin (Gibco cat# 15070063) ...
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bioRxiv - Biophysics 2024Quote: ... Purified protein samples were concentrated to 5-6 mg ml−1 in an aqueous buffer solution (20 mM Tris, 250 mM NaCl, 20% glycerol, and 10 mM 2- mercaptoethanol) and 0.6 M Trehalose (Merck KGaA, Darmstadt, Germany) when samples were lyophilized ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in 50 µl of water and thoroughly mixed at 1:1 volumetric ratio with matrix solution (2,5-Dihydroxybenzoic acid) (Merck KGaA, Darmstadt, Germany). One µl of each sample was deposited onto the MALDI (Matrix Assisted Laser Desorption Ionization ...
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bioRxiv - Cell Biology 2024Quote: ... cellular morphology was documented in HL-60 and K-562 by staining with 4-Nitro blue tetrazolium chloride (NBT) (James et al., 1997) () or Neutral Red solution (Merck/Sigma-Aldrich) (Russo et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096, Merck Millipore), and analyzed by pair-end sequencing using NovaSeq 6000 system (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR were performed in a 20ul reaction volume containing 10ul KAPA SYBR® FAST 2x mix (Merck), 0.4 μL ROX high (KAPA SYBR® FAST ...
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bioRxiv - Neuroscience 2020Quote: Rats were anesthetized by intraperitoneal administration of urethane (7.5 ml/kg loading dose and 0.3 ml repetitive dose for maintenance) from a 20% of weight urethane solution (Sigma-Aldrich / Merck KGaA, Darmstadt, Germany). The level of anaesthesia was monitored by checking whisker and pedal reflexes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Five milliliters of each standard solution was placed in a flat-bottom test tube for spectrophotometer analysis (Spectroquant Empty cells 16 mm; Merck KGaA, Darmstadt, Germany), which was also used for incubation ...
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bioRxiv - Neuroscience 2022Quote: ... until reaching the desired staining contrast, and cleared in two xylene baths (2 min, 20 min) before being coverslipped with an entellan-xylene solution (Merck KGaA, Darmstadr, Germany).
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was discarded and aliquots (20 μL) of the remaining solution (500 μL) were plated on solid Reasoner’s 2A (R2A; Sigma-Aldrich, Merck, Rahway, NJ, USA) to confirm the absence of bacterial growth seven days after incubation at 25°C.
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bioRxiv - Molecular Biology 2023Quote: ... Milli-Q water was used for preparing all aqueous solutions (18 MΩ×cm, <5 ppb, Millipore Milli-Q® Reference A+ system, Merck Millipore, Germany). LC-MS grade acetonitrile (ACN ...
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bioRxiv - Biophysics 2022Quote: ... equal amounts of GFP and SNAP-tagged recombinant WT-FUS protein (yielding a total protein concentration of 6 μM) were gently mixed into an aqueous solution of 20 % PEG-35 (Merck KGaA, Darmstadt, Germany) and 500 mM KCl (Merck KGaA ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Microbiology 2022Quote: ... Surviving animals were humanely euthanized at the end of the study using a commercial euthanasia solution (270 mg/kg sodium pentobarbital: Merck, Kenilworth, NJ, USA). C ...
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bioRxiv - Microbiology 2022Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
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bioRxiv - Molecular Biology 2024Quote: ... The plate was washed three times with TBS-C blocking solution and incubated with goat-anti mouse IgG-HRP (A4416, Merck; dilution 1/10,000) added in TBS-C blocking solution and incubated during 1 h at RT ...
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bioRxiv - Microbiology 2024Quote: For the carbon assays the minimal medium was supplemented with the RPMI-1640 amino acid solution (Merck, Cat. No. R7131; 50 mL/L) as nitrogen sources ...
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bioRxiv - Microbiology 2024Quote: ... and then for 1 hour in blocking solution containing primary antibodies (rabbit-anti-Slc1 (gift from Raphael Valdivia 79; 1:1000) or rabbit-anti-FLAG (Merck, F7425; 1:1000)) ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were placed in a humid chamber and cells were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse monoclonal anti-FLAG clone M2 (Merck F1804; 1:1000), rabbit monoclonal anti-KTN1 clone D5F7J (Cell Signaling Technology #13243 ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were rinsed with 100% methanol before being functionalised in an amino-silane solution (3% vol/vol (3-aminopropyl) triethoxysilane (Merck cat no. 440140), 5% vol/vol acetic acid (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was digested with restriction enzymes and then ligated into pET-17b (Merck Millipore, Burlington, MA, USA) to construct the expression vector pStSOR ...
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bioRxiv - Microbiology 2022Quote: ... hawaiiensis NRRL 15010 were obtained by ligating PCR-amplified gene sequences with linearized pET11a or pET22b vectors (Merck-Novagen), respectively ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was digested with XhoI and NcoI and introduced into the pET-28a vector (Merck Millipore). The ModA E165A ...
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bioRxiv - Immunology 2022Quote: ... The catalytically-inactive SPP D219A mutant was created using overlap-extension PCR (25) with KOD Hot Start Polymerase (Merck) and appropriate primers ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Microbiology 2023Quote: ... from a 50 mM biotin stock solution prepared in water with 60 mM NaOH (lyophilized biotin powder, 500 mg stock, Merck Life Science BV (Sigma)) ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a linezolid infusion bag was freshly prepared by diluting stock solution to the desired concentration in 0.9 % sodium chloride (Merck KGaA, Saint-Quentin-Fallavier, France). The concentration of the infusion solution depended on the dosing regimen (Tables S1 and S2) ...