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Citations for Merck :
751 - 800 of 4919 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and ESGRO-2i Supplement Kit (1:1000, #ESG1121; Merck KGaA)] in a humidified incubator at 37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... and visualized by exposing the membrane to a charge-coupled device camera (LAS1000, Fujifilm, Tokyo, Japan) using a chemiluminescence kit (Merck Millipore, Billerica, MA, USA). Membranes were stripped and reprobed for β-actin (diluted 1:50 000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were separated by SDS–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane (Hybond-P PVDF membranes, Merck, Germany) by a wet-transfer apparatus (Vep-3 ...
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Glutathione Sepharose 4B (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membrane (PVDF Transfer Membrane, Merck-Millipore). The primary antibodies used for the detection of proteins are Anti-CRY1 (Bethyl) ...
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bioRxiv - Immunology 2020Quote: ... All ELISAs were developed with 100 µl substrate solution per well containing 0.11 M sodium acetate (Merck), 0.1 mg/mL 3,5,3’,5’-tetramethylbenzidine (TMB ...
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bioRxiv - Neuroscience 2023Quote: ... Plasma ferritin was assessed in 324 samples via an ELISA (RAB0197-1KT, Merck Life Science UK Ltd). TBI was calculated using the below equation as proposed by Cook et al. ...
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bioRxiv - Neuroscience 2023Quote: ... We used the primary antibody of anti-PV mouse monoclonal antibody (1:1000, MAB1572, Merck Millipore) combined with the secondary antibody of anti-Mouse polyclonal goat antibody conjugated with Alexa Fluor 647 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were stained with different antibodies: mouse anti– rhodopsin 1D4 (1:1500, 1D4, Merck), rabbit anti-M-opsin (1:300 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Alexa Fluor 488-conjugated goat anti-mouse antibody (diluted at 1:200, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: mouse monoclonal antibody against actin (1:10,000, Merck Millipore, MAB1501R); mouse monoclonal antibody against PrP (POM1 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then first incubated with mouse anti-PSD95 antibodies (1:100; Merck-Millipore, Cat. #MAB1596) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... GAPDH (mouse; Merck), FLAG (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG (mouse; Merck), FLAG (rat ...
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... insulin and proglucagon were measured using the Luminex™ Mouse Metabolic Hormone Expanded kit (Merck & Co., Inc. Kenilworth, NJ). Also ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA was performed using Duolink In Situ Red Starter kit (Mouse/Rabbit) according to the manufacturer protocol (Merck, DUO92101).
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Thirty µl of Protein G (Protein G Sepharose Fast Flow, Merck, UK) slurry per sample was added in addition to 0.01% sodium azide and incubated on a roller overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Total protein concentration was quantified by means of BCA protein assay (Merck). 10-20 μg of protein was separated by size using a 4-12% Bis-Tris Plus gel ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A and Protein G conjugated to HRP were purchased from Merck. DNA dye Hoechst 33342 was purchased from ThermoFisher Scientific and used at a final concentration of 5 µg/ml.
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bioRxiv - Cell Biology 2019Quote: ... and transferred to PVDF membranes (Merck Millipore, Billerica, MA). All antibodies were diluted in Odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Developmental Biology 2020Quote: ... and transferred to Immobilon P membranes (Merck group, IVPD00010) using a semi-dry blotting system (Biometra/ Analytik Jena ...
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bioRxiv - Bioengineering 2022Quote: ... and electrotransferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). Membrane was cut between 80 and 110 kDa marker and at 50 kDa to probe simultaneously phospho or total MET ...
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bioRxiv - Molecular Biology 2019Quote: ... The sampes were filtered through nitrocellulose membrane (Merck-Millipore) layered on top of nylon Hybond N+ (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred to PVDF membranes (IPVH00010, Merck Millipore, Hertfordshire, UK) and immunoblotted with antibodies to phospho-S473 Akt (rabbit polyclonal ...
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bioRxiv - Molecular Biology 2019Quote: ... Elution was desalted on 0.025 µm VSWP membranes (Merck) against water for EMSAs.
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bioRxiv - Microbiology 2019Quote: ... and transferred to methanol-activated PVDF membranes (Merck, IPVH00010), using Western blot transfer buffer (3.03 g Tris ...
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bioRxiv - Biochemistry 2021Quote: ... 0.45 and 0.22-μm polyether sulfone membranes (Merck -Millipore) and finally diafiltered onto a polyether sulfone membrane with a 10kDa cut-off (Vivaspin ...
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bioRxiv - Microbiology 2020Quote: ... and polyvinylidene difluoride (PVDF) membranes were obtained from Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... and transferred to nitrocellulose membranes (Merck Millipore, Ltd-Ireland) in Buffer Tris 25 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... PVDF membranes were stained with Ponceau S stain (Merck) to confirm full transfer followed by blocking for 30 minutes at room temperature in 5% non-fat milk in PBST (0.01% Triton-X100) ...
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bioRxiv - Cell Biology 2022Quote: ... and transferred to polyvinylidene fluoride membranes (Merck Millipore, ISEQ00010). The membranes were blocked with 5% BSA in TBST for 1 hour ...