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Citations for Merck :
1001 - 1050 of 4919 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Mouse anti-NeuN antibody (Merck Millipore ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were digested by addition of porcine trypsin (enzyme to protein ratio of 1:100; for 2 x104 microglial samples, 300 ng was used; Merck) and incubated overnight at 37 °C with shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were incubated with anti-HA agarose beads (20 μl of beads per 1 mg of proteins) (Merck, Darmstadt, Germany) for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Microbiology 2023Quote: ... binding of equine antibodies was detected by incubation for 1 hour with protein A conjugated to peroxidase (GE) and visualized with chemiluminescence reagent (ECL, Merck). Images were obtained with an Odyssey LI-COR instrument ...
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bioRxiv - Biochemistry 2019Quote: Insulin and amylin concentration in plasma were determinate by ELISA (Insulin, Cat # EZRMI-13K and Amylin, Cat # EZHA-52K, Merck Millipore) following manufacturer manual ...
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Neuroscience 2022Quote: ... The animals used in our experiments were genotyped for expression of the transgene by quantitative PCR (qPCR) using the KAPA Mouse genotyping kit (Merck, Cat# MGKITKB-KK7301), with primer sequence (5’-3’ ...
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bioRxiv - Microbiology 2023Quote: ... SPN infected A549s were fixed with chilled methanol followed by further processing with Duolink® In Situ Orange Starter Kit Mouse/Rabbit (MERCK, DUO92102-IKT) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in PBS/ 0,05%Tween20 containing 5% skim milk and then probed with the following primary antibodies over night at 4°C: mouse anti-P53 (1:100, Merck), rabbit anti-H2AX (1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections containing SN and VTA were incubated with antibodies directed against tyrosine hydroxylase (mouse monoclonal, 1:1000, Merck-Millipore MAB318) and mCherry (rabbit polyclonal ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by washing and staining with horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG secondary antibodies (1:5,000 dilution; Merck Millipore), respectively ...
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bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN (1:200, MAB377X, Merck Millipore, MA, USA) or the rabbit polyclonal primary antibody against DISC1 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: Proximity ligation assays with Aurora A kinase and MP-GAP were performed in HeLa cells using mouse anti-Aurora A Kinase antibody (1:500, A1231 Merck), rabbit anti-MP-GAP antibody (1:250 ...
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight with an anti-GFP rabbit antibody (0.5 μg/ml) (Tamamaki et al., 2000) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000; MAB3120, Merck Millipore). After a rinse ...
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bioRxiv - Cell Biology 2023Quote: ... 1:10,000 WB) and mouse monoclonal antibody recognizing the N-terminus β-Actin (#A5441, 1:10,000 WB) were purchased from Merck. Rabbit polyclonal phosphomyosin (Thr18/Ser19 ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... or 14% SDS-PAGE gel and transferred onto PVDF membrane (Merck, ISEQ85R) using a semi-dry transfer unit (Bio-Rad Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... then transferred onto polyvinylidene fluoride membrane (IPVH00010, Merck Millipore, Billerica, MA, USA), blocked with 5% dry nonfat milk in Tris-buffered saline (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... separated by SDS-page and blotted onto a polyvinylidene difluoride membrane (Merck) by using a Wet/Tank Blotting System (Biorad) ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were blocked with 10% (W/V) skim milk (Merck, USA) and subsequently incubated with primary antibodies for 2 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were filtered through nitrocellulose (NC)-filter membranes (Merck Millipore HAWP 0025) at a rate of 7.5mL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... WB was performed with homemade SDS-PAGE gels and nitrocellulose membranes (Merck). The following antibodies were used ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to an Immobilon-FL PVDF membrane (Merck, Amsterdam, the Netherlands). The membrane was blocked for 1 hour at room temperature in 4% skim milk/PBS containing 0.1% Tween-20 ...
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bioRxiv - Microbiology 2019Quote: ... onto polyvinylidene fluoride membranes (Immobilon-P, 0.45 μm; Merck-Millipore, Darmstadt, Germany). Membranes were probed using anti-HA or anti-SiiA and anti-SiiB primary antibodies (as indicated in the respective figures ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to Immobilon-FL PVDF membrane (0.45 μm pore; Merck, IPFL00010). Membranes were incubated with 0.5% casein (Bio-rad ...
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bioRxiv - Cell Biology 2021Quote: ... blotted onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) via semi-dry blotting system and protein signal analyzed by using enhanced chemiluminescence to detect bound antibodies (WesternBrightTM ECL ...
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bioRxiv - Biochemistry 2021Quote: ... After transferring onto a polyvinylidene fluoride (PVDF) membrane (Merck KGaA, Darmstadt, Germany), bands on the membrane were visualized using Chemi-Lumi One Ultra (NACALAI TESQUE ...
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bioRxiv - Systems Biology 2020Quote: ... each well of the 96-well PVDF membrane plates (MSIPS4510, Merck Millipore) was activated and equilibrated with 150 μl of 70% ethanol/water and urea sample solution ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to PVDF Immobilon-P transfer membrane (Merck-Millipore, Darmstadt, Germany) as previously described (32) ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Microbiology 2020Quote: ... electrotransfered onto 0.2-µm Immobilon®-PSQ PVDF membrane (ISEQ00010, Millipore/Merck) and immunoblotting was done essentially as described above ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was developed with a peroxidase-conjugated secondary antibody from Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were incubated with Immobilon® Western Chemiluminescent HRP Substrate (Merck) and visualized and photographed by Tanon-5200 (Tanon ...