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Citations for Merck :
651 - 700 of 703 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Transwell migration/invasion assays were performed using PET membrane inserts (BRAND® 24-well Cell Culture Insert, PET-membrane, #BR782711, Merck) covered with 20 μL of Matrigel:PBS (1:2) ...
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bioRxiv - Cell Biology 2020Quote: ... The sections were subsequently dehydrated through a series of ethyl alcohol solutions and then placed in xylene before being coverslipped with Entellan mounting media (Merck Millipore, USA).
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bioRxiv - Immunology 2021Quote: ... wells washed with 1 X PBS and incubated with 50 µL of incomplete media containing 5 µM Laurdan (Avanti® Polar Lipids, Merck, USA) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... each media sample was concentrated to approximately 100ul using a centrifugal filter unit with a 3kDa cut-off (Merck Millipore, Cork, Ireland), digested with trypsin (2.5μg trypsin ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Bioengineering 2022Quote: ... – except for those sections using AEC as the visualization system which required an aqueous mounting media (Cat#108562, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Microbiology 2024Quote: ... The media were then filter-sterilised using a 0.22 μm Steritop® vacuum-driven disposable filtration system (Merck-Millipore, Burlington, MA, USA) prior to yeast inoculation.
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bioRxiv - Biochemistry 2024Quote: ... All media were supplemented with 10% v/v heat-inactivated (56 °C at 30 min) fetal calf serum (FCS) (Sigma/Merck, Darmstadt, Germany). Growth took place at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assessed by trypan blue exclusion and 3 × 106 viable cells from each condition were loaded into the upper chambers of Transwell culture inserts with a 0.5 µm pore size (Merck, Burlington, MA, USA). Cells were allowed to migrate towards the lower well containing medium ...
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bioRxiv - Microbiology 2021Quote: ... Clonal populations were obtained by limiting dilution and cell culture growth was monitored with an automatic Muse cell analyzer (Merck Millipore, Paris). Knockout and rescue cell lines were validated by whole-genome sequencing (BGI ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Retinal pieces were cultured on a cell culture insert with a 0.4 µm pore diameter (Millicell PICM0RG50, Merck Millipore Ltd, Cork, Ireland) in DMEM/Ham’s F-12 without Phenol Red (Nacalai Tesque ...
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Microbiology 2023Quote: ... Clonal populations were obtained by limiting dilution and cell culture growth was monitored with an automatic Muse cell analyzer (Merck Millipore, Paris).
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bioRxiv - Microbiology 2024Quote: ... a volume of 20 mL with an OD720 of 0.6 was withdrawn from the culture grown in the desired conditions and vacuum filtered through a Durapore® 0.45 µm PVDF membrane (Merck Millipore-Ireland). The filter with the biomass was immediately inverted into a petri dish with 1.6 ml of pre-cooled methanol for quenching and incubated at −80°C for an hour with periodic rubbing of the filter against the container to aid cell lysis ...
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bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
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bioRxiv - Microbiology 2024Quote: ... rivuli cultures were incubated with 1 mg of chitin suspended in 220 μL 1% PBS (phosphate buffered saline, Sigma Aldrich/Merck, US) in LC-MS grade water ...
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bioRxiv - Neuroscience 2023Quote: ... All plastic and glassware used to culture MPAs was coated with poly-L-lysine (PLL; 4µg/mL; catalogue # P1274, Merck Life Sciences (UK)) ...
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bioRxiv - Microbiology 2023Quote: ... inhibens were prepared through the following steps: Algal cell concentrations from a late exponential phase culture were enumerated using a CellStream CS-100496 flow cytometer (Merck, Darmstadt, Germany) with 561 nm excitation and 702 nm emission ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Biochemistry 2022Quote: ... Culture supernatants were harvested 24 h post-viral infection and then passed through 0.22 μM pore-sized filters (Merck Millipore, Carrigtwohill, Ireland) to remove cell and debris and obtain viral suspension for experiments.
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bioRxiv - Microbiology 2024Quote: ... 20 °C) from NO2- replete active cultures using Amicon Ultra-15 10 kDa cut-off centrifugal filter units (Merck Millipore, Darmstadt, Germany). Concentrated cells were washed with and resuspended in substrate-free medium (pH 6 ...
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bioRxiv - Microbiology 2024Quote: We plated 50μL of each co-culture mixture in triplicate on sterile 13mm Mixed Cellulose Ester (MCE) filter papers (pore size 0.22μm, Merck Life Science UK Ltd.), placed flat on pre-dried ...
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bioRxiv - Cancer Biology 2024Quote: ... The stock solution was added at 1% v/v to Plasmax cell culture medium (CancerTools cat# 156371) supplemented with 5% FBS (Merck cat# F7524) and penicillin/streptomycin (Gibco cat# 15070063) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL of culture was sonicated for 3 s and 200 μL were loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Merck Millipore) connected to the CellASIC® microfluidic pump (Merck Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... were confirmed mycoplasma-free and authenticated via short-tandem repeat (STR) analysis prior to general culture as previously.30 β-Catenin was stabilised using the GSK-3β inhibitor CHIR99021 (Merck-Millipore) as previously described,8 whilst transcription was inhibited via 2µg/ml Actinomycin D (Merck-Millipore ...
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Microbiology 2022Quote: ... and/or Escherichia coli (ATCC 11303) were acclimated in a gas tight bottles (100 ml) containing Luria Bertani Broth media (LB, Merck Millipore, BD, 0083370) under anaerobic conditions (∼0.2 mg L-1 O2 ...
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bioRxiv - Microbiology 2023Quote: ... 2× overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS/DMEM media and 0.6% (v/v) methylcellulose (Merck Life science, Cat: M0512) to achieve a semi-solid overlay ...
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bioRxiv - Cancer Biology 2024Quote: ... to obtain single cells and 1×104 cells were re-suspended in 1 mL of standard stem cell media comprising DMEM F12 (Merck, Cat. #D8437, Germany) supplemented with mouse basic fibroblast growth factor (mbFGF ...
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bioRxiv - Plant Biology 2020Quote: ... Conidia for either seeding liquid cultures or for use in pathogenicity assessments were collected by washing 4-week-old MM cultures with sterile water (SW, Milli-Q Integral Water Purification System for Ultrapure water, Merck KGaA, MA, USA), then filtered through glass wool to remove mycelial debris ...
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bioRxiv - Neuroscience 2021Quote: ... spheroids from each microwell were collected by firmly pipetting medium in the well up and down and transferred into Corning® non-treated culture dishes (Merck, CLS430591-500EA) in TeSR™-E6 (StemCell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were seeded in culture dishes and the specific pharmacological inhibitors radicicol (inhibitor of ATP-binding site of Hsp90, Sigma-Aldrich, Merck, Darmstadt, Germany), cyclosporine A (inhibitor of Cyp activity ...
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bioRxiv - Neuroscience 2023Quote: ... were cut on a McIlwain tissue chopper and transferred onto 30 mm diameter Millicell culture inserts with 0.4 mm pores (Merck, Fontenay sous Bois, France). Slices were maintained in culture in six-well-plates containing 1 mL of nutrient medium per well ...
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bioRxiv - Molecular Biology 2022Quote: ... EB from each microwell were collected by pipetting up and down the medium several times and transferred into Corning® non-treated culture dishes (Merck, CLS430591-500EA) in EB medium containing DMEM/F12 GlutaMAX (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... cells were cultured under standard cell culture conditions in FBS-free RPMI-1640 cell culture medium supplemented with 300 nM phorbol 12-myristate 13-acetate (PMA; Merck KGaA, Darmstadt, Germany) for 6 hours ...
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bioRxiv - Microbiology 2024Quote: The ability of the strains to grow with EA as a sole carbon source was tested on minimal media supplemented with 150 nmol 100% cyanocobalamin as vitamin B12 (Merck Life Science Limited, UK) and 30 mmol ≥ 99.0% EA hydrochloride (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... spores were collected from 5-day-old cultures NO3 medium (0.17% yeast nitrogen base, 3% sucrose, 100mM KNO3) by filtering through miracloth (Merck; pore size of 22–25μm). Spores were centrifuged ...
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bioRxiv - Developmental Biology 2020Quote: ... and in vitro culture in mineral oil covered drops (~20 μL/ ~15 embryos) of commercial KSOM (EmbryoMax® KSOM without Phenol Red; Merck, MR-020P-5F), conducted as previously described (Mihajlovic et al. ...
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bioRxiv - Cell Biology 2023Quote: Differentiation into the three germ layers was performed in suspension culture in Corning® Costar® Ultra-Low Attachment 6-well-plates (Merck, Darmstadt, Germany). Prior to seeding ...
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bioRxiv - Cell Biology 2023Quote: ... and after removing these contaminating cells the final cell suspension was plated into wells of an 8-well cell culture slide (Millicell® EZ Slides, Merck Millipore, Darmstadt, Germany) coated with human fibronectin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...