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Citations for Merck :
601 - 650 of 703 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 15 mL of the filtered culture supernatant was first concentrated using Amicon Ultra 15 mL Centrifugal Filters (cut-off 10 kDa, Merck), and the proteins were precipitated out of the solution using trichloroacetic acid (TCA ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were then transferred to cell-culture plastic plates that had been previously treated with poly-L-lysine 10 μg/ml (Sigma-Merck) to enhance cellular adhesion and promote monolayer cultures ...
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bioRxiv - Neuroscience 2024Quote: ... Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck) and 500 mM sodium chloride ...
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bioRxiv - Microbiology 2024Quote: ... LS-ARS2 overnight culture was inoculated (1% v/v) in MRS broth adjusted to pH 4 and pH 3 with HCl (1N, Merck), followed by incubation for 0 ...
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bioRxiv - Neuroscience 2024Quote: ACS was prepared from astrocyte cultures with aNSPC medium and filtered with a 3 kDa filter (Amicon Ultra-4 centrifugal Filter 3 kDa MWCO, Merck UFC8003243 to obtain a < 3 kDa and a > 3 kDa fraction ...
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bioRxiv - Cell Biology 2024Quote: ... 6 x 103 hESCs were seeded on mitotically inactivated MEF feeder in a well of 12-well plates and cultured in hESC culture medium for five days before subjected to alkaline phosphatase staining using Leukocyte Alkaline Phosphatase Kit (Merck) following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... the culture was stimulated by substituting one third of the medium with L929 medium (L929 mouse fibroblast cells had previously been plated in T175 cm2 cell culture flasks (Corning, Merck) with 100 mL culture medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Biochemistry 2024Quote: ... The cultures were incubated for 5 days at 30 °C and 130 rpm (Multritron, INFORS) using AeraSeal films (Merck, #A9224) on top of the flask ...
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bioRxiv - Biochemistry 2024Quote: First culture plates were coated with 200µL of poly-D-lysine (0.1mg/mL in dH2O, Merck-Sigma, Cat. No. P6407) and incubated overnight at 37°C in a 5% CO2 environment ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Cancer Biology 2020Quote: ... 72 hours conditioned-media from cancer cells was collected and filtered with a 0.45 μm filter (Millex, Merck Millipore, Massachusetts, USA) before adding to fibroblasts ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Immunology 2021Quote: ... weighed and then dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... method [68] with the following modification: Equal volumes of spent media were concentrated to approximately 50 μL with Microcon - 10 kDa centrifugal filter units (Merck Millipore) at a speed of 14,000 x g ...
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Synthetic Biology 2022Quote: Cells were grown in CellASIC ONIX microfluidic plates for haploid yeast cells in media controlled by the ONIX2 microfluidics system (Merck, Germany). Details regarding the composition of the media used for different promoter induction experiments are given in Supplementary Note 1.
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bioRxiv - Microbiology 2022Quote: ... Cell-free supernatant (CFS) was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Immunology 2024Quote: ... For analysis of splenic myeloid populations spleens were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... wadsworthia under various conditions was assessed by monitoring its OD600 after 48 hours of incubation on ABB media enriched with different electron phosphosulfate (APS) sodium salt (Merck, UK), and 10 mM taurine ...
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bioRxiv - Synthetic Biology 2024Quote: ... LCC and Fast PETase. Single colonies (E. coli XJb (DE3)) were cultured in 1 mL of overnight autoinduction media (Merck, USA) supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2024Quote: ... cells were picked from a single colony and grown overnight in -Ura synthetic minimal media (2 g/L Yeast Synthetic Drop-out Medium Supplement without uracil (Merck #1501), 67 g/L Yeast Nitrogen Base Without Amino Acids (Merck #Y0626) ...
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bioRxiv - Cell Biology 2024Quote: ... Medium in the capillary channel was then replaced with serum free endothelial cell media containing 40 mg/ml FITC-albumin (Merck, A9771), and the non-cellular channel medium replaced with serum-free RPMI ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue slices were dissected under the microscope in ice cold PBS before they were transferred on top of hydrophilic Millicell cell culture inserts (Merck Millipore) into serum-free Advanced Dulbecco’s modified Eagle’s medium F12 supplemented with 0.5% N2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lymphocytes were isolated from the spleen and incubated in a tissue culture tube at a cell concentration of 1 × 106/ml in RPMI1640 (Nacalai Tesque) containing lipopolysaccharide (10 μg/ml, Merck KGaA), concanavalin A (3 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... INS-1 cells in bioprinted grid scaffolds were stained with thiazolyl blue tetrazolium bromide (MTT) after 5 days in culture according to the manufacturer’s protocol (Merck, Darmstadt, Germany). Proliferation was determined using an ATP-based assay with luminescent readout (CellTiter-Glo® 3D Cell Viability Assay ...
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bioRxiv - Neuroscience 2022Quote: ... A sequence of coronal slices (400 μm) was cut at the level of the somatosensory cortex and plated on Millicell-CM organotypic culture membrane inserts (Merck Millipore). Slices were cultured in a medium containing 50% Optimem-1 ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Systems Biology 2022Quote: Yeast cell culture pellets were resuspended in lysis buffer composed of 100 mM triethylammonium bicarbonate (TEAB) (Merck Sigma, Cat. No. T7408) containing 0.1% ...
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bioRxiv - Developmental Biology 2022Quote: Dissected lung lobes were placed onto a hydrophilic polytetrafluoroethylene organ culture insert with a pore size of 0.4 µm (Merck Millipore, #PICM01250), which was preset in a 35 mm petri dish filled with 800 µL of culture medium ...
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bioRxiv - Neuroscience 2021Quote: ... These slices were explanted onto Millicell cell culture inserts (pore size 0.4 µm, diameter 30 mm, Merck Millipore Inc., cat# PICMORG50). Membrane inserts were placed into a six-well plate with 1 mL of culture medium ...
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bioRxiv - Bioengineering 2020Quote: All circuits were made on tissue-culture-treated surfaces – 60 or 35 mm circular dishes (Corning, Merck product 430166 or #430165) or rectangular uniwell plates with overall dimensions of 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Tumor slice and ear derma explants were then cultured on a 30-mm cell culture insert featuring a hydrophilic PTFE membrane (0.4 µm pore size, Merck Millipore #PICM0RG50) placed inside 6-well plates containing 1.1 mL of RPMI ...
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bioRxiv - Cancer Biology 2023Quote: ... For the in vitro functional experiments PDXs were maintained in culture in Iscove’s modified Dulbecco’s medium (IMDM, Sigma Aldrich-Merck, Cat#I3390) supplemented with 200 mmol/L L-glutamine (Euroclone ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM medium (Merck/Biochrom) supplemented with 10% HI-FBS (Merck/Biochrom) ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 to 200,000 HEK293-EBNA cells were plated in 1 mL complete DMEM per well of 12-well cell culture plates (#665180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: Astrocytes for long-term neuronal cultures were prepared by dissociating newborn rat cortices with 2.5% trypsin and 1 mg/ml DNase (Merck, cat# 10104159001) and plating the cells in MEM with L-glutamine supplemented with 0.6% glucose ...
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bioRxiv - Developmental Biology 2023Quote: ... coated culture plates in N2B27 basal medium containing 50:50 Dulbecco′s Modified Eagle′s Medium (DMEM) F12 (Merck Life Science) / Neurobasal medium (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... Viability analysis was performed by staining parasites in culture medium with 0.01 mg/ml of propidium iodide (P4864, Merck Life Sciences). For cell cycle and PAD1 expression analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered cell culture supernatant containing infectious HCV was concentrated 50x using 100 kDa Amicon Ultra-15 centrifugal filter units (Merck Millipore) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... AT2 Organoids were fixed with PFA for 3 minutes and then released from matrigel by incubating the culture with Corning Cell Recovery Solution (Merck, CLS354253) for 2 hours at 4 °C.
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bioRxiv - Microbiology 2024Quote: ... algal cells concentrations from a late exponential phase culture were counted by a CellStream CS-100496 flow cytometer (Merck, Darmstadt, Germany) using 561 nm excitation and 702 nm emission ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in a 10-cm-diameter tissue culture dish (IWAKI) with BIS medium under an anaerobic condition using Anaerocult® A (Merck). Trophozoites were detached from the surface of the dishes by adding cold PBS into the dishes and incubating them at 4℃ for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 200μL for 0.5% glucose added or 250μL for 0.4% glucose) of final expression cultures were centrifuged and pellets were lysed with 50 μL bugbuster (Merck, Rahway, NJ). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: Algal cell concentrations from a late exponential phase culture were enumerated using a CellStream CS-100496 flow cytometer (Merck, Darmstadt, Germany) with 561 nm excitation and 702 nm emission ...
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bioRxiv - Immunology 2024Quote: ... 48 or 72h in this culture medium additionally supplemented with either 0.1mM or 1mM Arg or Phe or Vehicle (Merck, Darmstadt, Germany). After incubation ...
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bioRxiv - Cell Biology 2024Quote: ... The cell culture medium was concentrated using Amicon® Ultra-15 3 K centrifugal filters (Merck Millipore Ltd., Burlington, MA, USA) into a final volume of 80 ml ...