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Citations for Merck :
601 - 650 of 1059 citations for Rat Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed using ice-cold RIPA buffer containing 1% of protease and phosphatase inhibitor cocktail (Sigma Aldrich/Merck, Poznan, Polska). The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing monomeric I-Ek/MCC(ANP)-biotin complexes were concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK), snap frozen in liquid nitrogen and stored in 1x PBS at −80 °C ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Plant Biology 2021Quote: ... The eluate containing the nanodiscs was concentrated to 1 ml with the use of an Amicon Ultra-15 10K device (Merck Millipore) and then mixed with 10 ml of translation buffer containing 30 mM HEPES-KOH (pH 7.8 ...
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bioRxiv - Neuroscience 2021Quote: ... The enzymatic digestion was stopped by adding a plating medium (Dulbecco’s modified Eagle medium, D5796, Merck, containing 10% fetal bovine serum). Tissues were dissociated by pipetting ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis seeds were surface sterilized with 1 mL 70% (v/v) ethanol containing 0.05% (v/v) Tween 20 (Merck, Darmstadt, Germany) by shaking on a table incubator at 1400 rpm for 3 min ...
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Neuroscience 2019Quote: ... detached cells were then added to phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA) to a final concentration of 10 μM and dissociated to single cells ...
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bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged GB1 and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to HEPES buffer containing 40 mM imidazole using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S75 Increase 10/300 column equilibrated in HEPES buffer without imidazole and a pH of 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged Mistics and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to Tris buffer containing 40 mM imidazole in an Amicon centrifugal filter (Merck Millipore) with adequate cutoff ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet obtained after the initial low-speed centrifugation was incubated in ice-cold IP buffer containing 1% NP-40 and 25-50 U of Benzonase (Merck, 70746) for 30 min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture medium was replaced with the medium of the same composition but without phenol red containing 100 μM D-Luciferin sodium salt (Merck, L6882). The source code (https://github.com/barta-lab/LuminoCell ...
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bioRxiv - Microbiology 2022Quote: Bacteria were enumerated from the lung homogenate and blood by dilution in PBS and plating onto solidified LB agar containing 50ug/ml rifampicin (Merck, UK).
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bioRxiv - Molecular Biology 2020Quote: ... all cell lines were seeded in black-walled 96 well plates at a density of 1×104 cells/well or and were maintained in the dark after induction of optogenetic receptor expression through 24 hours incubation in serum-free medium containing 100ng/ml doxycycline hyclate (Merck, #D9891). Optical stimulation was performed at room temperature using either a custom-built Arduino-controlled LED array device using 470nm LEDs (Ballister et al. ...
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bioRxiv - Plant Biology 2020Quote: ... tumefaciens were resuspended in 50 mM MES (2-Morpholinoethanesulfonic acid hydrate) (Duchefa, Haarlem, The Netherlands) - KOH buffer (pH 5.6) containing 2mM NaH2PO4 (Merck, Darmstadt, Germany), 100 µM acetosyringone (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... peptides were loaded onto an in-house zwitterionic Zic-HILIC SPE cartridge containing 20 mg of Zic-HILIC particles (10 μm, 200 Å; Sequant/Merck). The flow-through was collected and re-circulated through the column an additional three times ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Neuroscience 2021Quote: ... PBMCs were washed twice with PBS containing 1mM EDTA and subsequently frozen in 10% dimethyl sulphoxide (DMSO) (Merck KGaA, Hesse, Germany) and 90% foetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... Resulting lipid films were dissolved in 1 mL of n-hexane containing a C19:0 as internal standard (1.03 μg mL−1, CAS number 1731-94-8, Merck, Darmstadt, Germany) with addition of 200 μL of a solution of potassium hydroxide (KOH ...
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bioRxiv - Cell Biology 2021Quote: ... human iPSC cells were detached using Accutase for 5min at 37 °C and followed dissociated into single cells with phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA). Cells were centrifuged at 300 rpm for 5 min and the pellet was resuspended in hESC medium (20% knockout serum replacement ...
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bioRxiv - Microbiology 2020Quote: ... and the other stored at −80 °C in a sterile 2 ml cryovial containing 1 ml of a 30 % glycerol solution, prepared by diluting glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Immunology 2021Quote: ... weighed and then dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed by incubation with 2% Saponin in HBSS and lysates were serially diluted in 0.05% Tween-80 / dH20 and plated on 7H10 agar plates containing 10% heat-inactivated bovine serum (Merck, Darmstadt, Germany). Plates were incubated for 3 to 4 weeks at 37°C ...
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bioRxiv - Immunology 2022Quote: Cells were lysed by addition of a mild lysis buffer containing 1% octyl-β,D-glucopyranoside (#O9882-500MG, Sigma-Aldrich, Merck), 0.25% sodium deoxycholate (#1065040250 ...
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bioRxiv - Biophysics 2022Quote: ... 2 mL of EV-containing eluate was collected and concentrated to 100 μL using Amicon Ultra-2 10K filters (Merck Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... the experiments were performed in 96-wells plates containing 100 μL of MM (with 1% agar) supplemented or not with 3mM of H2O2 (Merck S.A) or 0.15mM of menadione (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing BirA were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
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bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing CLPB3 were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: J774A.1 macrophages with or without infection (3 hour) were lysed in a specific lysis buffer containing DCP-Bio1 (Merck # NS1226) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... and fractions containing histone octamers were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). The protein concentration was determined with Bio-Rad Protein Assay Dye Reagent (Bio-Rad # 5000006).
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bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2023Quote: ... coated culture plates in N2B27 basal medium containing 50:50 Dulbecco′s Modified Eagle′s Medium (DMEM) F12 (Merck Life Science) / Neurobasal medium (Gibco ...
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bioRxiv - Microbiology 2023Quote: Washed DPI-treated VBNC Mtb cells were resuspended with fresh Dubos broth and the suspension was diluted to 1:10 with Dubos broth with 0.1% (w/v) BSA containing adenylyl cyclase inhibitor SQ22536 (Merck, Darmstadt, Germany) or protein kinase inhibitor H89 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 10 pylorus and ileum sections from bees coming from the same cage were pooled in a 2 ml screw cap tube containing 750 μl of TRI reagent (Sigma-Aldrich, Merck), glass beads (0.75-1 mm diameter ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The AGA52 was grown in MRS broth (0.25% dextrose + 0.3% ox bile containing) supplemented with 100ppm cholesterol (5-cholesten-3β-ol (Sigma, Merck GmbH, Darmstadt, Germany), dissolved in 2-propanol ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Plant Biology 2023Quote: Plants were grown on 1/2 MS agar plates containing 0.01% (v/v) dimethyl sulfoxide (mock) or 50 ng mL-1 TM (654380; Merck, Darmstadt, Germany) for 14 days ...
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bioRxiv - Neuroscience 2023Quote: ... we transferred experimental and control 76 flies to a vial containing food covered with 20µl of all transretinal (ATR) solution (100mM ATR in 100 %ethanol; Sigma Aldrich R2500, Merck, Germany) and wrapped in aluminium foil.
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bioRxiv - Molecular Biology 2023Quote: ... filtered cell culture supernatant containing infectious HCV was concentrated 50x using 100 kDa Amicon Ultra-15 centrifugal filter units (Merck Millipore) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... Triton X-100 and incubated for 48 h in the sealed jars in the presence of a perforated tube containing vermiculite saturated with 7.43 g/100 mL KMnO4 solution (Merck, Darmstadt, Germany) as described by Ophir et al ...
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bioRxiv - Immunology 2024Quote: ... All Hoxb8-Fl cell lines were cultured in very low endotoxin containing RPMI 1640 (PAN-Biotech) supplemented with 10% FBS (Sigma-Aldrich/Merck), 2mM L-glutamine ...