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Citations for Merck :
551 - 600 of 720 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Reaction was started by the addition of 10 mM Nα-Benzoyl-L-arginine ethyl ester hydrochloride (BAEE, Merck). After 30 min the reaction was quenched with EDTA (50 mM final concentration) ...
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bioRxiv - Neuroscience 2023Quote: ... The 18-μm-thick cryostat tissue sections were incubated for 30 min in EdU reaction buffer (0.1 M Tris-HCl pH 7.0 (Cat. #: 648317, Merck), 4 mM CuSO4 copper (II ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR were performed in a 20ul reaction volume containing 10ul KAPA SYBR® FAST 2x mix (Merck), 0.4 μL ROX high (KAPA SYBR® FAST ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were later visualized by Coomassie staining and western blotting with anti-ubiquitin (Merck,#05-944, 1:5000) antibody or anti-His (GeneScript ...
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bioRxiv - Neuroscience 2024Quote: ... The reactions were monitored by thin layer chromatography on silica gel plates (60 F254, Merck, Prague, Czech Republic), and the spots were visualized by ultraviolet light (254 nm) ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were analysed using a reversed-phase C18 column (Chromolith™ C-18 column, Merck, Kenilworth, NJ, USA) and a linear gradient (solvent A ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were analyzed by Coomassie staining and western blotting with anti-ubiquitin antibody (Merck, #05-944, 1:5000) after separation on a 4-15% gradient SDS-PAGE gel.
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bioRxiv - Developmental Biology 2024Quote: ... In Situ Hybridization signal was visualized through a colour reaction by bathing slides in BM-Purple (Merck # 11442074001). The colour reaction was allowed to develop in the dark at room temperature during the appropriate amount of time and was stopped by bathing slides in 4% paraformaldehyde/PBS for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: Transfected HEK293T cells were treated for 16 h with DMSO or with 10 µM Cyclopiazonic acid (CPA, Merck) before collection.
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Organic acids (lactate, acetate, pyruvate and succinate) were analyzed using a reversed-phased HPLC (Merck-Hitachi, Darmstadt, Germany) equipped with a diode array detector (L-7450 ...
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bioRxiv - Cell Biology 2022Quote: ... The corresponding GST fusion protein was added to a final concentration of 15 µg/ml in incubation buffer (10 mM Tris pH 8.0, 150 mM NaCl, 0.1 % Tween-20, 3 % BSA (fatty acid free, Merck)) ...
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bioRxiv - Molecular Biology 2020Quote: ... and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v) (Merck, Roth) in a developing chamber (CAMAG ...
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bioRxiv - Biochemistry 2020Quote: ... One and a half μl of protein solutions were mixed on the target plate with 0.5 μl of the 20% 2,5-dihydroxybenzoic acid (Merck) solution in 20% ACN ...
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bioRxiv - Neuroscience 2020Quote: ... The larval samples were digested overnight at room temperature with concentrated (65%) nitric acid (Suprapur®grade, Merck) followed by 20 minutes at 90°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg of the total lysates were mixed with 1mL 0.1 mg/mL pepsin in 0.5M acetic acid (Merck) and put at 4°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The separation was carried out using a reversed-phase Eclipse plus C 18 column (1.8 μm, 2.1 × 50 mm) with corresponding solvent A [0.1 % formic acid (FA; Merck, USA) in water] and solvent B [0.1 % FA in acetonitrile (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... beta-galactosidase solution was prepared containing 20 mM citric acid (pH=6) (ref 1.00244.0500, Merck Millipore, Darmstadt, Germany), 5 mM of potassium hexacyano-ferrate (II ...
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bioRxiv - Molecular Biology 2024Quote: ... integrity was inspected on 1% agarose gel in TAE buffer pre-stained with GelRed nucleic acid stain (Merck).
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bioRxiv - Cell Biology 2023Quote: ... overnight cultures were grown at 30 °C at 200 rpm in Yeast Nitrogen Base without amino acids (Merck) prepared according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated in 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... prior to fixation embryonic zona pellucidae were removed in prewarmed (37°C) drops of Acid Tyrode’s solution (Merck) diluted in M2 ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples (200 mg) were digested in 4 ml of EMSURE ISO nitric acid 65% solution (Merck, #1.00456.2508) using MARS 6 microwave extraction system ...
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bioRxiv - Genetics 2024Quote: ... 200 ng was loaded onto Evosep tips according to manufacturer’s instructions and separated using the pre-defined 30 samples per day protocol in combination with an Evosep 150 mm x 0.15mm C18 reversed phase column (EV1106 endurance column packed with 1.9 µm C18AQ particles from Dr Maisch) using 0.1% formic acid (Merck) in ultra-pure water (Biosolve ...
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bioRxiv - Cancer Biology 2024Quote: ... The final concentrations of fatty acids used in the HFHS medium were: 150 µM palmitate (Merck cat# P5585), 150 µM stearic acid (Thermo Scientific cat# A12244.06) ...
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bioRxiv - Biochemistry 2024Quote: ... Phospho peptides were then reconstituted in 10 μl of MS loading buffer containing 0.3% Trifluoroactetic acid (TFA)(Merck) and 2% Acetonitrile (ACN ...
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bioRxiv - Biophysics 2024Quote: ... Modified amino acids Fmoc-Tyr(SO2ONp)-OH and Fmoc-Tyr(PO(OBzl)OH)-OH were purchased from Merck Life Science ...
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bioRxiv - Molecular Biology 2024Quote: ... air-dried at RT for 30 min and fixed in Methanol:Acetic acid in a 3:1 ratio (Merck) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were dissolved in 10% acetonitrile containing 0.1% formic acid and applied to a Ziptip (Merck Millipore) preconditioned with 95% acetonitrile containing 0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: ... no. 100246D) were from BDH Chemicals Ltd (Poole, England). Formic acid (>98%, cat. no. 1.00264.1000) was from Merck KGaA (Espoo ...
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bioRxiv - Bioengineering 2020Quote: All circuits were made on tissue-culture-treated surfaces – 60 or 35 mm circular dishes (Corning, Merck product 430166 or #430165) or rectangular uniwell plates with overall dimensions of 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product was run on an SDS gel and purified using the Agarose Gel DNA extraction kit from Merck (11696505001).
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bioRxiv - Microbiology 2024Quote: ... The PCR product was digested with NdeI and XhoI and cloned between the corresponding sites of pET-26b(+) (Merck, Darmstadt, Germany). The resulting plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was stopped in distilled water and slides were mounted with Fluoromount™ aqueous mounting medium (Merck F4680).
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bioRxiv - Microbiology 2020Quote: The TLC was performed by spotting 2 μL of enzymatic reaction on a silica gel 60 F454 plate (Merck), the separation was carried out in butanol ...
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bioRxiv - Immunology 2020Quote: ... The extracted fragment of interest was ligated into the T7Select® vector in a reaction (Merck KGaA, Darmstadt, Germany). The sample was added to a 1.5 mL EP tube ...
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bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
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bioRxiv - Biochemistry 2024Quote: Unless otherwise noted the standard assay was carried out at 65 °C for 10 min in 500 μL reaction mixture using 0.25 % (w/v) laminarin from Laminaria digitata (Merck) as substrate in 50 mM sodium phosphate buffer pH 6.0 (adjusted at assay temperature) ...
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bioRxiv - Physiology 2023Quote: ... For citrate synthase activity, 15-30 µg protein was added to the reaction buffer (20 mM Tris-HCl, pH 8.0 (Merck), 0.42 mM acetyl-coenzyme A (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... each probe (at a final concentration of 10 pM) was incubated in reaction buffer (5 mM CoCl2, 0.1 mM DIG-11-dUTP (Sigma Merck), 0.1 mM dATP ...
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bioRxiv - Neuroscience 2024Quote: ... The reactions were monitored by thin layer chromatography (TLC) on silica gel plates (60 F254, Merck, Prague, Czech Republic), and the spots were visualized by ultraviolet light (254 nm) ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% sodium dodecyl sulfate (SDS; AppliChem; Darmstadt, Germany) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... that was recently established by a disruption of both AAH1 and APT1.18 Yeast strains were grown overnight at 30 °C in a synthetic defined SD medium (6.7g/L Yeast Nitrogen Base without Amino Acids (Difco) and 20g/L glucose (Merck)) containing a specific mixture of amino acids and nucleobases ...
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bioRxiv - Cell Biology 2022Quote: ... resolubilized in 25 µL of 0.05% trifluoroacetic acid in 98:2 H2O/acetonitrile (v/v) and filtrated through a 0.2 µm spin filter (Merck Millipore Ultrafree®-MC ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
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bioRxiv - Bioengineering 2021Quote: ... The chemical reagents of L-cysteine (analytical reagent) and lactic acid (analytical reagent) were purchased from Merck (Darmstadt, Germany) and Fischer scientific (Merelbeke ...