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Citations for Merck :
501 - 550 of 720 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was digested with restriction enzymes and then ligated into pET-17b (Merck Millipore, Burlington, MA, USA) to construct the expression vector pStSOR ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was digested with XhoI and NcoI and introduced into the pET-28a vector (Merck Millipore). The ModA E165A ...
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bioRxiv - Cell Biology 2023Quote: ... Calibration curves were acquired using defined amounts of the fluorescent product 4-methylumbelliferone sodium salt (#M1508, Sigma-Aldrich/Merck). Fluorescence of 4-methylumbelliferone was normalized to crystal violet intensity.
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bioRxiv - Biophysics 2024Quote: ... The resulting product was then dialysed for 3h by using a membrane filter (MF-Millipore 0.025 µm membrane, Merck) and concentrated 10X using a speed vacuum concentrator ...
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bioRxiv - Biophysics 2022Quote: ... was dissolved in water and washed with the reaction buffer by using AmiconUltra-0.5 50K (Merck Millipore), and the concentration was quantified by Pierce BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... All reactions were monitored by thin layer chromatography (TLC) on silica gel plates (Keiselgel 60 F254, Merck) and/or ultra-performance liquid chromatography (UPLC) ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction buffer was exchanged to the lysis buffer five times by Amicon Ultra 10K (Merck Millipore). Then ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture (1 μl) was spotted onto the glass TLC plate (silica gel 60 F254, Merck) The TLC plates were developed appropriate times with the solution (isopropyl alcohol ...
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bioRxiv - Molecular Biology 2024Quote: ... The following antibodies were used for the nChIP reactions: rabbit mAbs α-H3K4me3 (1:200; Merck Millipore), α-H3K27me3 (1:300 ...
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bioRxiv - Genetics 2024Quote: ... The chromogenic reaction was carried out by incubating the larvae in BCIP/NBT solution (Merck, Cat. 203790) in NTMT buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was monitored by thin-layer chromatography (TLC) on silica-plated (silica gel 60-120, Merck) aluminum sheets ...
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bioRxiv - Plant Biology 2020Quote: Inflorescences were harvested into fresh fixative (3:1 96% [v/v] ethanol [Merck] and glacial acetic acid) and kept overnight (O/N ...
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bioRxiv - Biophysics 2020Quote: ... Then His-tag containing OpuAC was introduced to the flow cell (in buffer B supplemented with 10 mM of (±)6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; Merck) as a photostabilizer[20] ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid extracts were pooled and an aliquot of samples and standard mix (triolein:diolein:monoolein:oleic acid) were applied to a silica 60 thin-layer chromatography plate (1.05553.0001, Merck, Darmstadt, Germany) using hexane:diethylether:acetic acid (70:29:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and functionalized by 200 µl of a 3% (v/v) solution of hyaluronic acid (HA) (Merck, Germany) at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The mobile phase consisted of (A) 0.1% formic acid in MQ H2O (Merck Millipore, Bedford, MA, USA) and (B ...
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bioRxiv - Immunology 2021Quote: ... pH 5 (0.1 M citric acid monohydrate from Sigma and 0.2 M disodium phosphate dihydrate from Merck)) was added to the plate and incubated for 30 minutes in the dark ...
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bioRxiv - Immunology 2020Quote: ... MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA, Merck, Whitehouse Station, NJ). Peptides were purified by ultrafiltration using centrifugal filter units (Amicon ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.25% sodium deoxycholate and 0.1% SDS (AppliChem) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II and IV (Merck) ...
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bioRxiv - Immunology 2022Quote: ... The loading pump delivered a solution of 0.1% formic acid in 98% water / 2% acetonitrile (Merck, Germany) at 10 µL/min ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed and permeabilized with a 3:1 mixture of methanol (Klinipath)-glacial acetic acid (Merck) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 million cells were dissolved into 100 µl of ICP-OES grade 65% HNO3 acid (Merck #1.00441.1000) and was kept at 95°C for overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were maintained in Neurobasal/B27 supplemented with 20 ng/ml L-ascorbic acid (Merck Life Sciences), 20 ng/ml BDNF (PreproTech ...
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bioRxiv - Genetics 2024Quote: ... For PAS staining the sections were stained with 0,9 % periodic acid (cat# 3257.1, Roth) and Schiffsches Reagent (cat#1.09033, Merck) both for 10 min embedded into washing steps with H2O ...
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bioRxiv - Plant Biology 2023Quote: Exogenous hormone treatments comprised 5 μM 1-napthaleneacetic acid (NAA; Merck Life Science UK Ltd., Gillingham, UK) (0.1% v/v Tween20) ...
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bioRxiv - Immunology 2022Quote: ... ATP and Poly(deoxyadenylic-thymidylic) acid sodium salt (Poly dA-dT) were obtained from Merck (Darmstadt, Germany). Val-boroPro - Calbiochem 5314650001 was obtained from Merck (Darmstadt ...
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bioRxiv - Bioengineering 2023Quote: ... The mobile phase was 1.5 mmol L-1 phosphoric acid diluted in ultrapure water (MilliQ, Merck Millipore). The VFAs measured were acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Incubations were terminated after 90 mins by the addition of 0.2 ml 20% perchloric acid (Merck Millipore) and ...
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bioRxiv - Cell Biology 2023Quote: ... and fertilized eggs were cultured in EmbryoMax KSOM Medium (1X) w/ 1/2 Amino Acids (Merck Millipore) at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were treated with 0.5% Periodic acid for 5 min and stained in Schiff’s reagent (Merck) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Depletion of Top2α was achieved by addition of 500μM of indole acetic acid (IAA) (Merck; I5148-2G) for 1h ...
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bioRxiv - Biochemistry 2024Quote: ... supplied with one tablet of ethylenediaminetetraacetic acid free SigmaFAST protease inhibitor cocktail tablet (Merck KGaA, Darmstadt, Germany) per 100 ml buffer ...
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bioRxiv - Biochemistry 2024Quote: ... a one-step gradient elution was achieved with elution buffer (0.1 M citric acid, Merck, pH 2.5). Resulting fractions were neutralized immediately with 1 M Tris (Serva ...
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bioRxiv - Biophysics 2024Quote: ... with or without pre-treatment with 500 μM L-Ascorbic acid (Vitamin C) (Sigma-Aldrich/Merck, #A4544) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM ethylenediaminetetraacetic acid (EDTA)) using an ultra-centrifugal filter (Amicon ultra-4 [30 kDa]; Merck). The (His)6-tag was cleaved by reacting with HRV3C overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... product T7451; used at 1/500) and CHMP1B (rabbit polyclonal antibody, ATLAS product HPA061997; used at 1/500) were from Merck; CHMP2A (rabbit polyclonal ...
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bioRxiv - Microbiology 2021Quote: Reaction products and standards were dissolved in 20% 1-propanol and separated on a 10 cm HPTLC Si-60 plates (Merck) using 1-propanol:acetone:water 9:6:4 (v:v:v ...
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bioRxiv - Synthetic Biology 2021Quote: ... GGA reactions were performed in a thermocycler according to a standard GGA protocol.52 The GGA products were transformed into NovaBlue cells (Merck), from which the plasmids were purified using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned using the hot fusion method (Fu et al., 2014) in pET30a+ vector (Merck, Molsheim France) pre-digested with EcoRI and BglII in order to fusion AgLTP24 with a N-terminal 6XHis flag ...
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bioRxiv - Developmental Biology 2023Quote: ... Product number E4250) (1 mg/ml) to contract pigment cells alongside 0.01% Tricaine (Ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich (Merck) Product number E10521 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were stopped by separating the enzyme and soluble products from the insoluble chitin substrate by filtration using 96-well filter plates (Merck). The concentration of oxidized LPMO products was also determined in aliquots taken from the pre-incubation reaction with β-chitin to allow accounting for carry-over of products potentially formed in the pre-incubation phase.
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bioRxiv - Molecular Biology 2020Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore).
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bioRxiv - Plant Biology 2020Quote: Extracts of AkPT1 reaction mixtures were chromatographically separated on a LiChrosphereRP-18 column (4.0 mm × 250 mm, Merck) at a flow rate of 1.0 ml min-1 and at 40 °C under the control of a D-2000 Elite HPLC system (Hitachi ...
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bioRxiv - Physiology 2021Quote: ... Reactions were monitored either by thin-layer chromatography (carried out on silica plates, silica gel 60 F254, Merck) and visualized under UV light ...
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bioRxiv - Microbiology 2021Quote: ... divided into two reaction tubes and parasites were incubated either with 125 µg/ml TPCK-treated trypsin (Merck) or with 1xPBS at 37°C for 45 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... ligation for 30 min 37 °C and polymerase reaction overnight at 37 °C according to manufacturer’s protocol (Merck, Duolink® In Situ Detection Reagents Red ...
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bioRxiv - Biochemistry 2023Quote: ... Reaction was started by the addition of 10 mM Nα-Benzoyl-L-arginine ethyl ester hydrochloride (BAEE, Merck). After 30 min the reaction was quenched with EDTA (50 mM final concentration) ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).