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Citations for Merck :
5751 - 5800 of 5854 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1:100, in-house antibody SCV-1E8 (Morgan et al., 2023)) in conjunction with either anti-NeuN (chicken, 1:3000, Merck, ABN91), anti-Iba1 (rabbit ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... ACN and then rehydrated with 30 µL of 3.33 µg × mL-1 trypsin (cat-no. T6567 from Sigma-Aldrich/Merck, www.sigmaaldrich.com) in 25 mM ABC ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
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bioRxiv - Immunology 2024Quote: ... For analysis of splenic myeloid populations spleens were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by approx ...
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bioRxiv - Cancer Biology 2024Quote: ... in T-BST for 1 h at room temperature or Re-Blot Plus Strong Antibody Stripping solution for 15 min at room temperature (Merck, #2504). Quantification of protein bands was performed using ImageJ analysis software (release 1.53c).
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The chromatin pellet was then digested in 100 μl of water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 min at 37°C in a thermomixer at 1,400 rpm.
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bioRxiv - Cancer Biology 2024Quote: ... The shRNA mediated knockdown of the respective EWSR1::ETS fusion oncogenes was induced by addition of 1 μg/mL dox (Merck, Germany) to the cell culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Thawed Matrigel was diluted 1/50 in cold E8 media and was then added to the cell culture dishes (Merck, #Z755923). Coated dishes were incubated at room temperature for 1 hour and used immediately or kept in the fridge for later application.
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bioRxiv - Neuroscience 2024Quote: ... fish were briefly rinsed in water and embedded in 1% agarose (Carl Roth, Germany) in 1.8 ml vials (Nunc CryoTube Vials, Merck (Sigma-Aldrich), Germany) ...
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bioRxiv - Biophysics 2020Quote: mamM CTD gene from Magnetospirillum gryphiswaldense MSR-1 (UniProt Q6NE57 residues 215-318) was previously cloned into pET28a(+) vector (Novagen, Merck Biosciences, Germany)22 ...
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bioRxiv - Biochemistry 2019Quote: A CRISPR array containing three Mtb repeat sequences flanking two identical spacer sequences targeting the pUC19 multiple cloning site was assembled by BamHI digest of the double-stranded mtbCRISPR array oligonucleotide (Table S2) followed by ligation into pCDFDuet™-1 (Novagen, Merck Millipore). Mtb cas6 was cloned into MCS-2 to give pCRISPR.
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Genomics 2021Quote: ... The cloned shRNAs against the respective EWRS1-ETS fusion oncogenes was induced by addition of 1 µg/ml dox (Merck, Darmstadt, Germany) to the cell culture medium ...
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bioRxiv - Bioengineering 2021Quote: ... and the remaining skin was cut in strips of approximately 4 cm length and 1 mm width and finally incubated in 10 ml skin digestion solution (2.0 U/mL dispase D4693, Merck KGaA, in PBS-) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were processed with 1% H2O2 in phosphate buffer to deactivate the intrinsic peroxidase and then incubated with an anti-c-Fos antibody (1:10,000, rabbit; Merck KGaA, Darmstadt, Germany) in 10% normal goat serum in phosphate-buffered saline with 0.3% Triton X-100 (PBS-X ...
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then reprobed with monoclonal anti-β-actin-peroxidase clone AC-15 antibody (a3854, Merck, Saint Louis, MO, dilution 1:5000) in TBS/T for 90 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Immunostaining was performed on retinal cryosections by incubating with rabbit primary antibodies against cone arrestin (1:1000; catalog AB15282, Merck Chemicals GmbH), mouse anti-rhodopsin (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253, Darmstadt, Germany), 1:25000 diluted primary mouse monoclonal anti-beta actin antibody (AC-15 ...
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bioRxiv - Biochemistry 2022Quote: ... A BglII-XbaI fragment containing His8-MBP-NOT10 was then cut out of the pnEK vector and cloned between the BglII and AvrII restriction sites behind the second T7 promoter of the pETDuet-1 vector (Merck, Darmstadt, Germany). The resulting plasmid was cut with SalI and BglII ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Systems Biology 2022Quote: ... the proteins were alkylated for 30 min or 1 hour at room temperature in the dark by addition of 15 mM iodoacetamide (Merck Sigma, I1149) or 50 mM acrylamide (AA ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds 1-10 were purified by flash chromatography on high-purity grade silica gel (9385, 60 Å, 230-400 mesh) from Merck (Germany), producing hydrochlorides in the form of white powders.
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bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 mL of fixed cell suspension was filtrated by 0.22-µm pore-sized black polycarbonate filter (type GTBP; Merck Millipore, Darmstadt, Germany) and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with amino acid-free and FBS-free DMEM (Wako Pure Chemical Industries, 048-33575) to induce autophagy by starvation or to DMEM with 1 µM oligomycin (Merck Millipore, 495455) and 2 µM antimycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: General transformation procedure as described procedure A but using RbCl chemo-competent BL21(DE3) pLysS (aliquot of 100 µl; 2.4 × 107 cfu µg−1 pUC19 DNA, Merck Millipore, Darmstadt, DE). Transformed cells were plated (50 µl final volume ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were probed with anti-GFP at a dilution of 1:3000 of the antibody conjugated to a peroxidase (SAB2702198, Merck KGaA, Germany) in the blocking buffer overnight at 4 °C on a rocker ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... The proteasome inhibitor MG132 was used at 1 µM for 24 h (stock solution: 10 mM in DMSO, 474791, Sigma Aldrich/Merck KGaA). For all inhibitors ...
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bioRxiv - Immunology 2021Quote: ... Cartilage was dissected from the tibial plateau and minced before overnight collagenase digestion in DMEM-F12 supplemented with 1% P/S and 1mg/ml collagenase IA (Sigma, supplied by Merck, Darmstadt, Germany). Synovium was minced before being collagenase digested for 2 hours ...
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bioRxiv - Plant Biology 2022Quote: ... disposable 1000 μl pipette tips were fitted with 4 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1 mg:10 μg of LiChroprep® RP-18 (Merck) ...
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bioRxiv - Zoology 2023Quote: ... about 10 mL of CLE flower extract was added to a 90 mL aqueous solution of 1 mM AgNO3 (Merck Laboratories, India) and kept at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant containing the MCR-1 solubilized in DDM detergent micelles was filtered using an 0.22 µm syringe filter (Merck Millipore, Darmstadt, Germany) and applied onto a HisTrap HP 5 ml affinity chromatography column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa S3 Flp-In host cells were co-transfected with pEF5/FRT/CTLA4-HA and pOG44 at a ratio of 1:9 using GeneJuice (Merck Millipore, 70967) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/l, VWR; di-ammonium citrate 0.7 g/l, Sigma-Aldrich; sodium acetate 0.26 g/l, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/L, VWR; di-ammonium citrate 0.7 g/L, Sigma-Aldrich; sodium acetate 0.26 g/L, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... GFP signals were amplified by immunostaining with polyclonal rabbit anti-GFP (used at 1:500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, SAB4301138). 40-1a (used at 1:50 ...
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bioRxiv - Biophysics 2023Quote: ... Amphipol-reconstituted P2X4 was pooled and concentrated to approximately 1 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All steps were performed at 4 °C.
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...